Cofilin determines the migration behavior and turning frequency of metastatic cancer cells

We have investigated the effects of inhibiting the expression of cofilin to understand its role in protrusion dynamics in metastatic tumor cells, in particular. We show that the suppression of cofilin expression in MTLn3 cells (an apolar randomly moving amoeboid metastatic tumor cell) caused them to extend protrusions from only one pole, elongate, and move rectilinearly. This remarkable transformation was correlated with slower extension of fewer, more stable lamellipodia leading to a reduced turning frequency. Hence, the loss of cofilin caused an amoeboid tumor cell to assume a mesenchymal-type mode of movement. These phenotypes were correlated with the loss of uniform chemotactic sensitivity of the cell surface to EGF stimulation, demonstrating that to chemotax efficiently, a cell must be able to respond to chemotactic stimulation at any region on its surface. The changes in cell shape, directional migration, and turning frequency were related to the re-localization of Arp2/3 complex to one pole of the cell upon suppression of cofilin expression

Change and Exchange: Economies of Literature and Knowledge in Early Modern Europe

The introductory essay outlines the way in which Change and Exchange places literature, and, in a wider sense, imaginative practice, at the centre of early modern economic knowledge. Probing the affinity between economic and metaphorical experience in terms of the transactional processes of change and exchange, it sets up the parameters within which the essays in the volume collectively forge a language to grasp early modern economic phenomena and their epistemic dimensions. It prepares the reader for the stimulating combination of materials that the book presents: the range of generic contexts engendered by emergent economic practices, structures of feeling and modes of knowing made available by new economic relations, and economies of transformation in discursive domains that are distinct from ‘economics’ as we understand it but cognate in their intuition of change and exchange as shaping agents

An Evening with Jon Amon and Friends (November 7, 2014)

Macedonian Dance (2011) / Traditional, arr. Goran Daskalov Muses (1990) / Marie-Hélène Fournier Muse Verte Muse Climatique Muse Rhythmique Muse Épicée Jon Amon, saxophone Sequenza VIIb (1969/1993) / Luciano Berio, ed. Claude Delangle Ariflex 35 (2010) / Claudio Gabriele Dennis Shafer, Saxophone Sonatine (1903-1905) / Maurice Ravel I. Modéré II. Mouvement de menuet III. Animé Amy McGlothlin, saxophone Beverly Soll, piano Mai (1975) / Ryo Noda Amy McGothlin, saxophone Sonata IV (1738) / Georg Philipp Telemann Vivace ma moderato Piaceuole non Largo Presto Jon Amon, saxophone Amy McGothlin, saxophone If I’m Lost--Now (2013) / Wei Dai Jon Amon, alto saxophone Dennis Shafer, baritone saxophone Kimberly Ayers, voicehttps://vc.bridgew.edu/fac_concerts/1010/thumbnail.jp

Pseudopodium dynamics and rapid cell movement in Dictyostelium Ras pathway mutants

Loss of either of the Ras pathway members RasS or GefB causes growing Dictyostelium cells to move aberrantly rapidly. In this study, we describe the changes in motility that underlie these phenotypes using computer-assisted 3D dynamic image analysis. Unexpectedly, the two mutants use different mechanisms to achieve rapid migration. The rasS− cells' motility is characterised by highly dynamic cell morphology, with rapidly extending and retracting pseudopodia. The gefB− cells do not have an unusually dynamic morphology, and achieve their efficient translocation by the continual remodelling of an existing dominant anterior pseudopodium. In spite of these dramatic changes in pseudopodium behaviour, the underlying motility cycle of both mutants remains normal. The levels of F-actin in both mutant cell lines are significantly elevated with respect to the wild-type parental cells, suggesting a possible biochemical basis for these emphatic phenotypes

Lichens and mosses on shrub-steppe soils in southeastern Washington

The purpose of this study was to identify the lichens and mosses found on soils of the shrub-steppe at the Hanford Site in southeastern Washington. Twelve sites between 133 and 447 m and one at 807 m elevation were intensively sampled. Twenty-nine lichens and six moss species were identified. Three lichens were considered undescribed species. Based on comparison with other studies and herbarium records, we conclude the soil lichen flora of the Hanford Site is substantially different than that of the Great Basin or of the shrub-steppe in Idah

Sharing Data to Accelerate Medicine Development and Improve Neonatal Care: Data Standards and Harmonized Definitions

SCOPUS: no.jDecretOANoAutActifinfo:eu-repo/semantics/publishe