Interplay between gut lymphatic vessels and microbiota

Lymphatic vessels play a distinctive role in draining fluid, molecules and even cells from interstitial and serosal spaces back to the blood circulation. Lymph vessels of the gut, and especially those located in the villi (called lacteals), not only serve this primary function, but are also responsible for the transport of lipid moieties absorbed by the intestinal mucosa and serve as a second line of defence against possible bacterial infections. Here, we briefly review the current knowledge of the general mechanisms allowing lymph drainage and propulsion and will focus on the most recent findings on the mutual relationship between lacteals and intestinal microbiota

Mutations in Mycobacterium tuberculosis Isolates with Discordant Results for Drug-Susceptibility Testing in Peru

Evaluation of resistance to antituberculosis drugs is routinely performed with genotypic or phenotypic methods; however, discordance can be seen between these different methodologies. Our objective was to identify mutations that could explain discordant results in the evaluation of susceptibility to rifampicin and isoniazid between molecular and phenotypic methods, using whole genome sequencing (WGS). Peruvian strains showing sensitive results in the GenoType MTBDRplus v2.0 test and resistant results in the proportions in the agar-plaque test for isoniazid or rifampin were selected. Discordance was confirmed by repeating both tests, and WGS was performed, using the Next Generation Sequencing methodology. Obtained sequences were aligned "through reference" (genomic mapping) using the program BWA with the algorithm "mem", using as a reference the genome of the M. tuberculosis H37Rv strain. Discordance was confirmed in 14 strains for rifampicin and 21 for isoniazid, with 1 strain in common for both antibiotics, for a total of 34 unique strains. The most frequent mutation in the rpoB gene in the discordant strains for rifampicin was V170F. The most frequent mutations in the discordant strains for isoniazid were katG R463L, kasA G269S, and Rv1592c I322V. Several other mutations are reported. This is the first study in Latin America addressing mutations present in strains with discordant results between genotypic and phenotypic methods to rifampicin and isoniazid. These mutations could be considered as future potential targets for genotypic tests for evaluation of susceptibility to these drugs.Revisión por pare

Avaliação de genótipos de Avena sativa L. quanto a produção de forragem na região da Campanha/RS.

CONGREGA 2012

Effectiveness of the spot-on combination of moxidectin and imidacloprid (Advocate®) in the treatment of ocular thelaziosis by Thelazia callipaeda in naturally infected cats

Background: The present study evaluated the therapeutic effectiveness of moxidectin 1.0% (w/v) and imidacloprid 10% (w/v) (Advocate® spot-on solution for cats, Bayer Animal Health) against natural infections with the eyeworm Thelazia callipaeda in cats. This study was conducted as a GCP, negative-controlled, blinded and randomised field study in privately owned cats living in an area in southern Italy where T. callipaeda is enzootic. Methods: The study was carried out in 30 cats (19 females and 11 males, aged from 8 months to 5 years, weighing 1.2-5.2 kg) of different breeds, naturally infected by T. callipaeda. At study inclusion (Day 0), animals were physically examined and the infection level was assessed by examination of both eyes for clinical score and live adult T. callipaeda count. Each cat was weighed and randomly assigned to one of the treatment groups (G1: Advocate, G2: untreated control). Clinical assessments and T. callipaeda adult counts were performed on Day 14. At the study completion visit on Day 28, clinical assessments and counts of T. callipaeda adults and larvae were performed. All cats were daily observed by their owners and general health conditions were recorded during the entire period of the study. Results: The primary effectiveness variable was the percentage of animals in G1 group (Advocate) showing a complete elimination (parasitological cure) of adult eye worms at Day 14 and Day 28. The effectiveness of the treatment in the G1 group was 93.3 and 100% at Day 14 and Day 28, respectively, when compared to group G2. Total worm count reduction from both eyes for Advocate was 96.3% on Day 14 and 100% on Day 28. Clinical data were confirmed by the examination of conjunctival pouch flushing. An overall reduction in the number of cats with lacrimation and conjunctivitis was observed following treatment despite the fact that in a few cats treated with Advocate clinical signs persisted due to the chronic nature of the disease. Conclusions: Based on the results of the present trial, a single dose of Advocate was found to be safe and highly effective in the treatment of natural T. callipaeda infection in cats

Wind Tunnel Experimentation on Stationary Downbursts at WindEEE Dome

In the context of the European Project THUNDERR a scientific collaboration between the Wind Engineering and Structural Dynamics (Windyn) Research Group of the University of Genoa (Italy) and the Wind Engineering, Energy and Environment (WindEEE) Research Institute of Western University (Canada) has been established to study experimentally at the WindEEE Dome facility how the main geometrical and mechanical properties of downbursts are affected by different cloud base outflows of stationary thunderstorms. At present, the analysis of the downbursts simulated experimentally is ongoing and some preliminary elaborations have been obtained concerning the qualitative and quantitative interpretation of the corresponding signals. Classical signal decomposition was applied to experimentally produced downbursts in the WindEEE Dome in order to study transient features of the time series. This study presents the results for two radial positions from downdraft centre and for twenty repetitions per radial position. Several prospects for further research are also discussed

Geometric View of Measurement Errors

The slope of the best fit line from minimizing the sum of the squared oblique errors is the root of a polynomial of degree four. This geometric view of measurement errors is used to give insight into the performance of various slope estimators for the measurement error model including an adjusted fourth moment estimator introduced by Gillard and Iles (2005) to remove the jump discontinuity in the estimator of Copas (1972). The polynomial of degree four is associated with a minimun deviation estimator. A simulation study compares these estimators showing improvement in bias and mean squared error

A novel approach to scaling experimentally produced downburst-like impinging jet outflows

Downbursts are intense thunderstorm winds that can be found in most, if not all, regions around the globe. An accurate experimental investigation of downburst winds requires the proper geometric and kinematic scaling between the model downburst (m) created in a wind simulator and the full scale downburst event (p). This study makes a threefold contribution to further understanding of downburst outflows. First, the article introduces a new scaling methodology for downburst outflows based on the signal decomposition techniques of p and m downburst wind records. Second, the study describes a large set of m downbursts produced in the WindEEE Dome simulator at Western University and critically discusses their similarity with a large set of p events detected in the Mediterranean. Third, using the proposed scaling methodology, this paper attempts to partially reconstruct two p downburst events recorded in Genoa and Livorno, Italy. In total, 17 p and 1400 m downburst outflows are investigated herein, which represents the largest database of p and m downbursts combined. The similarity between p and m downbursts is quantitatively demonstrated for both mean and fluctuating components of the flows. The scaling method is verified by accurately predicting the known anemometer height of p events using m downburst measurements

Integration of satellite interferometric data in civil protection strategies for landslide studies at a regional scale

Multi-Temporal Satellite Interferometry (MTInSAR) is gradually evolving from being a tool developed by the scientific community exclusively for research purposes to a real operational technique that can meet the needs of different users involved in geohazard mitigation. This work aims at showing the innovative operational use of satellite radar interferometric products in Civil Protection Authority (CPA) practices for monitoring slow-moving landslides. We present the example of the successful ongoing monitoring system in the Valle D’Aosta Region (VAR-Northern Italy). This system exploits well-combined MTInSAR products and ground-based instruments for landslide management and mitigation strategies over the whole regional territory. Due to the critical intrinsic constraints of MTInSAR data, a robust regional satellite monitoring integrated into CPA practices requires the support of both in situ measurements and remotely sensed systems to guarantee the completeness and reliability of information. The monitoring network comprises three levels of analysis: Knowledge monitoring, Control monitoring, and Emergency monitoring. At the first monitoring level, MTInSAR data are used for the preliminary evaluation of the deformation scenario at a regional scale. At the second monitoring level, MTInSAR products support the prompt detection of trend variations of radar benchmarks displacements with bi-weekly temporal frequency to identify active critical situations where follow-up studies must be carried out. In the third monitoring level, MTInSAR data integrated with ground-based data are exploited to confirm active slow-moving deformations detected by on-site instruments. At this level, MTInSAR data are also used to carry out back analysis that cannot be performed by any other tool. From the example of the Valle D’Aosta Region integrated monitoring network, which is one of the few examples of this kind around Europe, it is evident that MTInSAR provides a great opportunity to improve monitoring capabilities within CPA activities