Prevalence of Fimeria funduli (Protozoa: Eimeriidae) in the Longnose Killifish Fundulus similis from Horn Island, Mississippi

Eimeria funduli occurred in Fundulus similis from Horn Island, Mississippi, during 1980, in contrast with its apparent absence during 1978 and 1979. Prevalence of the parasite appears to be related to the unusually low salinity in Mississippi Sound, and in ponds and off the beaches of Horn Island. The low salinity may have promoted the migration of infective intermediate hosts from inshore waters to the island

Evaluation of Flow-Through, Static, and Recirculating Systems for the Intensive Culture of the Gulf Killifish Fundulus grandis with Observations on a Solar-Heated Recirculating System for the Bait Industry

Three systems (flow-through, static, and recirculating) for intensive culture of the Gulf killifish (Fundulus grandis) for bait were evaluated. The outdoor recirculating system proved most successful. Killifish maintained in this system attained an average weight of over 2.0 gm in 42 days, an acceptable market size. Whereas the solar-heated recirculating system sustained fish densities equivalent to 1,000,000/ha and a survival rate of 86%, growth of killifish in this system was less than that observed in both the static and outdoor recirculating systems. Algae appeared to be an important nutritional component of the diet of juvenile killifish. Individuals of F. grandis fed diets supplemented with algae grew better than those maintained without them

Preliminary Observations of the Effect of Eimeria funduli (Protozoa: Eimeriidae) on the Gulf Killifish Fundulus grandis and Its Potential Impact on the Killifish Bait Industry

Liver infections of Eimeria funduli, a pathogenic coccidium, greatly affected growth of Fundulus grandis (Gulf killifish) maintained in an aquaculture facility for 55 days. At termination of the experiment, average weight gained by uninfected killifish was 877.5% compared to 308.0% in diseased fish. There appeared to be no correlation between mortality and disease in this study

A Selected Bibliography on the Mass Propagation of Rotifers with Emphasis on the Biology and Culture of Brachionus plicatilis

The rotifer Brachionus plicatilis has shown great promise as food for artificially reared fish and crustacean larvae. A list of references on mass propagation of rotifers is presented alphabetically by author. Citations cover and are indexed as culture, diet, ecology, physiology, population dynamics, reproduction, systematics, geographical distribution, food, and biology of rotifers with emphasis on Brachionus plicatilis

Prevalence of Fimeria funduli (Protozoa: Eimeriidae) in the Longnose Killifish Fundulus similis from Horn Island, Mississippi

Eimeria funduli occurred in Fundulus similis from Horn Island, Mississippi, during 1980, in contrast with its apparent absence during 1978 and 1979. Prevalence of the parasite appears to be related to the unusually low salinity in Mississippi Sound, and in ponds and off the beaches of Horn Island. The low salinity may have promoted the migration of infective intermediate hosts from inshore waters to the island

Is Enrichment Always Enriching and How Would You Know? Unintended Consequences and the Importance of Formal Assessment of Enrichment Programs in Bottlenose Dolphins (Tursiops truncatus)

Bottlenose dolphins (Tursiops truncatus) are viewed as a highly intelligent species capable of complex behaviors. This requires marine parks to maintain dynamic environmental enrichment programs in order to ensure dolphins’ optimal psychological and physiological well-being while in human care. In this study, two experiments were conducted to determine the effects of different forms of enrichment on the behavior of four bottlenose dolphins. In Experiment 1, multiple forms of novel enrichment resulted in a shift away from individual swim patterns – a change that is associated with increased behavioral diversity and so often considered an improvement in animal welfare – but also resulted in avoidance behavior and initially resulted in a decrease in affiliative behavior. In Experiment 2, introducing choice of enrichments resulted in unintended social consequences, such as agonistic behaviors. These two experiments together demonstrated that interpreting the results of enrichment programs may not be as straightforward as often presumed. The results suggest that unique forms of enrichment and variable schedules might be particularly effective but also that consistent evaluation continues to be necessary to minimize unintended behavioral consequences

Bartonella species detection in captive, stranded and free-ranging cetaceans

We present prevalence of Bartonella spp. for multiple cohorts of wild and captive cetaceans. One hundred and six cetaceans including 86 bottlenose dolphins (71 free-ranging, 14 captive in a facility with a dolphin experiencing debility of unknown origin, 1 stranded), 11 striped dolphins, 4 harbor porpoises, 3 Risso's dolphins, 1 dwarf sperm whale and 1 pygmy sperm whale (all stranded) were sampled. Whole blood (n = 95 live animals) and tissues (n = 15 freshly dead animals) were screened by PCR (n = 106 animals), PCR of enrichment cultures (n = 50 animals), and subcultures (n = 50 animals). Bartonella spp. were detected from 17 cetaceans, including 12 by direct extraction PCR of blood or tissues, 6 by PCR of enrichment cultures, and 4 by subculture isolation. Bartonella spp. were more commonly detected from the captive (6/14, 43%) than from free-ranging (2/71, 2.8%) bottlenose dolphins, and were commonly detected from the stranded animals (9/21, 43%; 3/11 striped dolphins, 3/4 harbor porpoises, 2/3 Risso's dolphins, 1/1 pygmy sperm whale, 0/1 dwarf sperm whale, 0/1 bottlenose dolphin). Sequencing identified a Bartonella spp. most similar to B. henselae San Antonio 2 in eight cases (4 bottlenose dolphins, 2 striped dolphins, 2 harbor porpoises), B. henselae Houston 1 in three cases (2 Risso's dolphins, 1 harbor porpoise), and untyped in six cases (4 bottlenose dolphins, 1 striped dolphin, 1 pygmy sperm whale). Although disease causation has not been established, Bartonella species were detected more commonly from cetaceans that were overtly debilitated or were cohabiting in captivity with a debilitated animal than from free-ranging animals. The detection of Bartonella spp. from cetaceans may be of pathophysiological concern

Biology and Pathogenesis of the Coccidium \u3ci\u3eEimeria funduli\u3c/i\u3e Infecting Killifishes

Epizootics of Eimeria funduli involved estuarine killifishes (Fundulus grandis, F. pulvereus, F. similis, and F. heteroclitus) in Mississippi, Alabama, and Virginia. All of more than 500 specimens examined of F. grandis from Mississippi during 1977 through 1979 had infections, regardless of age, sex, or season collected. Oocysts occurred primarily in the liver and pancreas, replacing up to 85% of both those organs. Infrequent sites of infection were fatty tissue of the body cavity, ovary, intestine, and caudal peduncle. Living fish did not discharge oocysts. Eimeria funduli is the first known eimerian to require a second host. To complete the life cycle, an infective stage in the grass shrimp Palaemonetes pugio had to be eaten. In 6-mo-old killifish reared in the laboratory at 24 C, young schizonts were first observed in hepatic and pancreatic cells 5 days post feeding, followed by first generation merozoites by day 10, differentiation of sexual stages during days 15 to 20, fertilization between days 19 and 26, sporoblasts from days 25 to 30, and sporozoites about day 60. Unique sporopodia developed on sporocysts by day 35 when still unsporulated. Temperatures of 7 to 10 C irreversibly halted schizogony. Both schizogony and sporogony progressed slower as age of host increased. When infective shrimp in doses ranging from 1 to 10% of a fish\u27s body weight were eaten, the level of intensity of resulting infections did not differ significantly. Pathogenesis followed a specific sequence, with the host response apparently unable to contend with extensive infections as seen typically in nature and in our experiments. Premunition was indicated. When administered Monensin® orally, infected fish exhibited a reduction in oocysts by 50 to 70% within 20 days as compared with untreated fish. Furthermore, infected killifish maintained exclusively on a diet of TetraMin® for 3 mo completely lost their infections

A New and Unusual Eimerian (Protozoa: Eimeriidae) from the Liver of the Gulf Killifish, \u3ci\u3eFundulus grandis\u3c/i\u3e

Oocysts and sporocysts of Eimeria funduli sp. n. are described from the Gulf killifish, Fundulus grandis, on the basis of light microscopy, transmission and scanning electron microscopy, and location in the liver of infected hosts. The spherical sporulated oocysts of E. funduli isolated from liver tissue measure 20-31(25) μm across with ovoid sporocysts 9-11 × 5-7 (10 × 6) μm. A micropyle, polar granule, and oocyst residuum are absent, but sporocysts have Stieda and substieda bodies, a few residual granules, and 10-25 (15) unique projecting structures with expanded distal portions that we term “sporopodia.” Sporopodia 1-3 (2) μm high support a transparent membrane that completely surrounds the sporocyst. Sporozoites have one large posterior refractile body. Ultrastructurally, the oocyst wall consists of two thin layers of granular material: an electron-dense outer layer with a rough external surface and an electron-lucent inner one of approximately equal thickness. One or two unit membranes line the inner surface of the inner layer. Each layer is 40-60(55) nm thick. The sporocyst wall, 78-130 (110) nm thick, consists of an electron-lucent material with the outer surface being more electron dense and giving rise to osmiophilic sporopodia; closely associated with these and the outer surface are one or two unit membranes. A thin osmiophilic layer of fine granular material lines the inner surface

A Filamentous Bacterium on the Brine Shrimp and Its Control

A strain of a colorless, filamentous bacterium (tentatively identified as Leucothrix mucor) heavily infests the brine shrimp, Artemia salina. Its ultrastructure, unlike that of some other strains, does not reveal a distinct middle layer between its outer cell wall layer and cytoplasmic membrane, irregular blebs extending from the cell layers, or an external sheath. An entire infestation, represented as a mat of the bacterium with associated debris and microorganisms, sloughs from the shrimp when exposed to a variety of treatments. Primarily because most effective treatments are toxic to the shrimp, 100 ppm terramycin provides the treatment of choice