Antimicrobial Activity of Varthemia iphinoides and Majorana syriaca Essential Oils from Jordan and Their Potential Use as Natural Food Preservatives

The aim of this study was to evaluate the efficiency of Varthemia iphinoides and Majorana syriaca essential oils as natural food preservative in four different food model media (cheese, meat, milk, and tomato) against two gram-positive and two gram-negative bacteria (Bacillus cereus, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa) and three different food spoilage fungi using agar well diffusion method. The results showed that the maximum activity of V. iphinoides essential oil was against S.aureus in tomato media with relative inhibition zone diameter (RIZD) 186 ± 9 %, the most sensitive food spoilage fungi was penicillium sp. in tomato media with RIZD 149 ± 10 %. The results showed that there was inhibitory effect of M. syriaca and V. iphinoides essential oils. against most of the tested species in different food model media which will lead to control food pathogen organisms by using essential oils as food preservative and flavoring agents. Key words: Varthemia iphinoides , Majorana syriaca, Essential oils, Antimicrobial, Food preservative

BACTERIAL PATHOGEN IN URINARY TRACT INFECTION AND ANTIBIOTIC RESISTANCE PATTEERN IN ZARAQA -JORDAN

This study aimed to isolate the pathogenic agents involving Urinary Tract Infections (UTI) in Zarqa city and to evaluate their antibiotic resistance pattern. Bacterial isolation and identificaction was conducted using standard methods. From 3756 urine sample collected from patients with UTI symptoms 392 (10.4 %) show positive urine cultures of which 318 (81 %) were females and 74 (19 %) were males. Among the isolated pathogen E. coli was the most frequent bacteria (80.8%) folloewd by Klebsiella spp. (11.7%), while Staphylococcus aureus was the least frequent bacteria (0.08%). All the isolated pathogens resist one or more antibiotic, E. coli and Klebsiella spp possessed high resitence to ampicillin (84.18 and 89.13%) respectively. One E. coil (0.32) isolate showed resistant against all tested antibiotics. Gram-negative bacteria were the major isolates in this study, and E. coli was the predominant isolated bacteria. The knowledge of antibiotic sensitivity pattern in this study will be helpful for the effective treatment of the UTI in this population

Synthesis, characterization and antimicrobial activity of new chalcones and their 6-aryl-4-(2,5-dichlorothiophen-3-yl)-6<i>H</i>-1,3-thiazin-2-amine derivatives

<p></p> <p>Chalcones (<b>5a-h</b>) were prepared in high yields <i>via</i> the condensation of 3-acetyl-2,5-dichlorothiophene (<b>3</b>) with aryl aldehydes (<b>4a-h</b>). The reaction of the resulting chalcones with thiourea in the presence of a catalytic amount of hydrochloric acid provided the 1,3-thiazin-2-amine derivatives (<b>6a-g</b>) in moderate yields. All new compounds were characterized by extensive NMR analysis data, including 1D NMR experiments (¹H and ¹³C) and 2D NMR experiments (COSY, HMBC, HSQC), as well as ESIMS and HRESIMS data. The newly synthesized compounds were evaluated for their biological activities, which demonstrate a very good to low bioactivities as antibacterial and antifungal, respectively.</p