Monoterpenoids dithiophosphates. Synthesis and biological activity

O,O-Dialkyldithiophosphoric acids adds at the double bond of the racemic camphene and (+)- limonene in the presence of Lewis acids in accordance with the Markownikoff rule with the formation of Sterpenyl esters of dithiophosphoric acids. The reaction with camphene is accompanied by the rearrangement of camphane structure to that of bornane. Addition of dithiophosphoric acid to (+)-limonene proceeds with the participation of the exocyclic double bond. Toxic and genotoxic properties of the monoterpenoid dithiophosphates were studied. © Pleiades Publishing, Ltd., 2010

Deciphering Heterogeneity in Pig Genome Assembly Sscrofa9 by Isochore and Isochore-Like Region Analyses

Background: The isochore, a large DNA sequence with relatively small GC variance, is one of the most important structures in eukaryotic genomes. Although the isochore has been widely studied in humans and other species, little is known about its distribution in pigs. Principal Findings: In this paper, we construct a map of long homogeneous genome regions (LHGRs), i.e., isochores and isochore-like regions, in pigs to provide an intuitive version of GC heterogeneity in each chromosome. The LHGR pattern study not only quantifies heterogeneities, but also reveals some primary characteristics of the chromatin organization, including the followings: (1) the majority of LHGRs belong to GC-poor families and are in long length; (2) a high gene density tends to occur with the appearance of GC-rich LHGRs; and (3) the density of LINE repeats decreases with an increase in the GC content of LHGRs. Furthermore, a portion of LHGRs with particular GC ranges (50%–51 % and 54%–55%) tend to have abnormally high gene densities, suggesting that biased gene conversion (BGC), as well as time- and energy-saving principles, could be of importance to the formation of genome organization. Conclusion: This study significantly improves our knowledge of chromatin organization in the pig genome. Correlations between the different biological features (e.g., gene density and repeat density) and GC content of LHGRs provide a uniqu

Community evaluation of glycoproteomics informatics solutions reveals high-performance search strategies for serum glycopeptide analysis

Glycoproteomics is a powerful yet analytically challenging research tool. Software packages aiding the interpretation of complex glycopeptide tandem mass spectra have appeared, but their relative performance remains untested. Conducted through the HUPO Human Glycoproteomics Initiative, this community study, comprising both developers and users of glycoproteomics software, evaluates solutions for system-wide glycopeptide analysis. The same mass spectrometrybased glycoproteomics datasets from human serum were shared with participants and the relative team performance for N- and O-glycopeptide data analysis was comprehensively established by orthogonal performance tests. Although the results were variable, several high-performance glycoproteomics informatics strategies were identified. Deep analysis of the data revealed key performance-associated search parameters and led to recommendations for improved 'high-coverage' and 'high-accuracy' glycoproteomics search solutions. This study concludes that diverse software packages for comprehensive glycopeptide data analysis exist, points to several high-performance search strategies and specifies key variables that will guide future software developments and assist informatics decision-making in glycoproteomics

РЕАЛИЗАЦИЯ ИДЕИ ПРИНЦИПА ПРИРОДОСООБРАЗНОСТИ В ЛЕТНЕМ ЭКОЛОГИЧЕСКОМ ЛАГЕРЕ ШКОЛЬНИКОВ

The goal: theoretical basis and experimental verification principle naturalism to the formation of ecological consciousness of pupils by using different forms of education and training in the summer environmental camp, as an important component of the additional environmental education. Method or the methodology of the work: the concept of ecological education (N.D. Andreev, I.N. Ponomareva, I.T. Suravegina, A.N. Drunk, Zoya Ivanova, etc.), additional research in environmental education (N.V. Dobretsova, E.V. Tutynin, R.P. Sofronov, etc.) and traditional environmental public education (G.N. Volkov, V.X. Harutyunyan Sh.A. Mirzoev, K.D. Utkin, A. Grigoriev, O.D. Mukaeva, Frenkel and others), including - Yakut (G.N. Volkov, V.F. Afanasyev, N.D. Neustroev, A.A. Grigoriev, D. Danilov, I.S. Portnyagin, W.A. Vinokourov, A.P. Okoneshnikova, E.P. Zhirkov, K.S. Chiryaev etc.), problems of moral education (I.F. Kharlamov, M.I. Shilov, F.D. Tovarischeva etc.). Results: Maturity of students' eco-ethical standards of conduct and responsible attitude to the environment based on the principle of naturalism. The area of application of the results: secondary school, institution of additional environmental education students (summer environmental camp); methods of teaching biology and ecology at the university.Цель: теоретическое обоснование и экспериментальная проверка принципа природосообразности к формированию экологического сознания школьников посредством использования различных форм обучения и воспитания в летнем экологическом лагере, как важного компонента системы дополнительного экологического образования. Методология проведения работы: концепция экологизации образования (Н.Д. Андреева, И.Н. Пономарева, И.Т. Суравегина, А.Н. Захлебный, З.И. Иванова, и др.), исследования в области дополнительного экологического образования (Н.В. Добрецова, Е.В. Тутынина, Р.П. Софронов и др.) и традиционного экологического народного воспитания (Г.Н. Волков, В.X. Арутюнян, Ш.А. Мирзоев, К.Д. Уткин, А.А. Григорьева, О.Д. Мукаева, Я.И. и др.), в том числе - якутского (Г.Н. Волков, В.Ф. Афанасьев, Н.Д. Неустроев, А.А. Григорьева, Д.А. Данилов, И.С. Портнягин, У.А. Винокурова, А.П. Оконешникова, Е.П. Жирков, К.С. Чиряев, Р.П. Софронов и др.)., проблемы нравственного воспитания (И.Ф. Харламов, М.И. Шилова, Ф.Д. Товарищева и др.). Результаты: сформированность у школьников эколого-нравственных норм поведения, ответственного отношения к окружающей среде на основе принципа природосообразности. Область применения результатов: общеобразовательная школа, учреждения системы дополнительного экологического образования школьников (летний экологический лагерь); методика обучения биологии и экологии в вузе

Monoterpenoids dithiophosphates. Synthesis and biological activity

O,O-Dialkyldithiophosphoric acids adds at the double bond of the racemic camphene and (+)- limonene in the presence of Lewis acids in accordance with the Markownikoff rule with the formation of Sterpenyl esters of dithiophosphoric acids. The reaction with camphene is accompanied by the rearrangement of camphane structure to that of bornane. Addition of dithiophosphoric acid to (+)-limonene proceeds with the participation of the exocyclic double bond. Toxic and genotoxic properties of the monoterpenoid dithiophosphates were studied. © Pleiades Publishing, Ltd., 2010

Monoterpenoids dithiophosphates. Synthesis and biological activity

O,O-Dialkyldithiophosphoric acids adds at the double bond of the racemic camphene and (+)- limonene in the presence of Lewis acids in accordance with the Markownikoff rule with the formation of Sterpenyl esters of dithiophosphoric acids. The reaction with camphene is accompanied by the rearrangement of camphane structure to that of bornane. Addition of dithiophosphoric acid to (+)-limonene proceeds with the participation of the exocyclic double bond. Toxic and genotoxic properties of the monoterpenoid dithiophosphates were studied. © Pleiades Publishing, Ltd., 2010

Monoterpenoids dithiophosphates. Synthesis and biological activity

O,O-Dialkyldithiophosphoric acids adds at the double bond of the racemic camphene and (+)- limonene in the presence of Lewis acids in accordance with the Markownikoff rule with the formation of Sterpenyl esters of dithiophosphoric acids. The reaction with camphene is accompanied by the rearrangement of camphane structure to that of bornane. Addition of dithiophosphoric acid to (+)-limonene proceeds with the participation of the exocyclic double bond. Toxic and genotoxic properties of the monoterpenoid dithiophosphates were studied. © Pleiades Publishing, Ltd., 2010