Highlights of the BioTM 2010 workshop on advances in bio text mining

This meeting report gives an overview of the keynote lectures, the panel discussion and a selection of the contributed presentations. The workshop was held in Gent, Belgium on May 10-11. It featured a tutorial aimed towards a broad audience of (computational) biologists, (computational) linguists and researchers working purely on text mining

The validation of Short Interspersed Nuclear Elements (SINEs) as a RT-qPCR normalization strategy in a rodent model for temporal lobe epilepsy

Background : In gene expression studies via RT-qPCR many conclusions are inferred by using reference genes. However, it is generally known that also reference genes could be differentially expressed between various tissue types, experimental conditions and animal models. An increasing amount of studies have been performed to validate the stability of reference genes. In this study, two rodent-specific Short Interspersed Nuclear Elements (SINEs), which are located throughout the transcriptome, were validated and assessed against nine reference genes in a model of Temporal Lobe Epilepsy (TLE). Two different brain regions (i.e. hippocampus and cortex) and two different disease stages (i.e. acute phase and chronic phase) of the systemic kainic acid rat model for TLE were analyzed by performing expression analyses with the geNorm and NormFinder algorithms. Finally, we performed a rank aggregation analysis and validated the reference genes and the rodent-specific SINEs (i.e. B elements) individually via Gfap gene expression. Results : GeNorm ranked Hprt1, Pgk1 and Ywhaz as the most stable genes in the acute phase, while Gusb and B2m were ranked as the most unstable, being significantly upregulated. The two B elements were ranked as most stable for both brain regions in the chronic phase by geNorm. In contrast, NormFinder ranked the B1 element only once as second best in cortical tissue for the chronic phase. Interestingly, using only one of the two algorithms would have led to skewed conclusions. Finally, the rank aggregation method indicated the use of the B1 element as the best option to normalize target genes, independent of the disease progression and brain region. This result was supported by the expression profile of Gfap. Conclusion : In this study, we demonstrate the potential of implementing SINEs-notably the B1 element as a stable normalization factor in a rodent model of TLE, independent of brain region or disease progression

Kainic acid-induced status epilepticus decreases mGlu5 receptor and phase-specifically downregulates Homer1b/c expression

Globally, over 50 million people are affected by epilepsy, which is characterized by the occurrence of spontaneous recurrent seizures. Almost one-third of the patients show resistance to current anti-epileptic drugs, making the exploration of new molecular targets necessary. An interesting target may be Homerl, due to its diverse roles in epileptogenesis and synaptic plasticity. Indeed, Homerl regulates group I metabotropic glutamate (mGlu) receptors (i.e. mGlu(1) and mGlu(5)) scaffolding and signaling in neurons. In the present work, using the systemic kainic acid (KA)-induced status epilepticus (SE) model in adult rats, we investigated the mRNA and protein expression patterns of the mGlu5 receptor, Homerla and Homerlb/c at 10, 80 and 120 days post-SE (i.e. T10, T80 and T120). Epileptogenesis was validated by electrophysiological recordings of seizures via electroencephalography (EEG) monitoring and through upregulation of glial fibrillary acidic protein. At the protein level, the mGlu5 receptor was downregulated in the late latent phase (T10) and the early- and late exponential growth phase (T80 and T120, respectively), which was best observed in the hippocampal CA1 region. At mRNA level, significant downregulation of the mGlu5 receptor was only detected in the late exponential growth phase. Homerla expression did not change at any investigated time point. Interestingly, Homerlb/c was only downregulated in the late latent phase, a period where spontaneous seizures are extremely rare. Thus, this phasespecific downregulation may be indicative of an endogenous neuroprotective mechanism. In conclusion, these results suggest that Homerlb/c may be an interesting molecular target to prevent epileptogenesis and/or control seizures

Biplaire stoornis in de huisartsenpraktijk:: huisartsen volgen de evidence

status: publishe

Nieuws uit de vakgroep : CTSE gaat voor duurzame polymeer- en textielinnovatie

Bij CTSE (Centre for Textile Science and Engineering) geloven we dat de sleutel tot succesvolle innovatie van duurzame textiel- en polymeermaterialen een combinatie is van fundamenteel en toegepast onderzoek naar nieuwe milieuvriendelijke materialen en productieprocessen, met het oog op performante eindproducten met minimale milieuimpact. Onze innovatieaanpak steunt op drie pijlers: analyseren, optimaliseren en samenwerken. We geloven sterk dat dit de enige manier is om (samen) antwoorden te vinden op de vragen van morgen

Clinical vagus nerve stimulation paradigms induce pronounced brain and body hypothermia in rats

Vagus nerve stimulation (VNS) is a widely used neuromodulation technique that is currently used or being investigated as therapy for a wide array of human diseases such as epilepsy, depression, Alzheimer's disease, tinnitus, inflammatory diseases, pain, heart failure and many others. Here, we report a pronounced decrease in brain and core temperature during VNS in freely moving rats. Two hours of rapid cycle VNS (7s on/18s off) decreased brain temperature by around 3 degrees C, while standard cycle VNS (30 s on/300 s off) was associated with a decrease of around 1 degrees C. Rectal temperature similarly decreased by more than 3 degrees C during rapid cycle VNS. The hypothermic effect triggered by VNS was further associated with a vasodilation response in the tail, which reflects an active heat release mechanism. Despite previous evidence indicating an important role of the locus coeruleus-noradrenergic system in therapeutic effects of VNS, lesioning this system with the noradrenergic neurotoxin DSP-4 did not attenuate the hypothermic effect. Since body and brain temperature affect most physiological processes, this finding is of substantial importance for interpretation of several previously published VNS studies and for the future direction of research in the field