Binary superlattice design by controlling DNA-mediated interactions

Most binary superlattices created using DNA functionalization or other approaches rely on particle size differences to achieve compositional order and structural diversity. Here we study two-dimensional (2D) assembly of DNA-functionalized micron-sized particles (DFPs), and employ a strategy that leverages the tunable disparity in interparticle interactions, and thus enthalpic driving forces, to open new avenues for design of binary superlattices that do not rely on the ability to tune particle size (i.e., entropic driving forces). Our strategy employs tailored blends of complementary strands of ssDNA to control interparticle interactions between micron-sized silica particles in a binary mixture to create compositionally diverse 2D lattices. We show that the particle arrangement can be further controlled by changing the stoichiometry of the binary mixture in certain cases. With this approach, we demonstrate the abil- ity to program the particle assembly into square, pentagonal, and hexagonal lattices. In addition, different particle types can be compositionally ordered in square checkerboard and hexagonal - alternating string, honeycomb, and Kagome arrangements.Comment: 4 figures in the main text. 5 figures in the supplementary informatio

Omnidirectional Sensory and Motor Volumes in Electric Fish

Active sensing organisms, such as bats, dolphins, and weakly electric fish, generate a 3-D space for active sensation by emitting self-generated energy into the environment. For a weakly electric fish, we demonstrate that the electrosensory space for prey detection has an unusual, omnidirectional shape. We compare this sensory volume with the animal's motor volume—the volume swept out by the body over selected time intervals and over the time it takes to come to a stop from typical hunting velocities. We find that the motor volume has a similar omnidirectional shape, which can be attributed to the fish's backward-swimming capabilities and body dynamics. We assessed the electrosensory space for prey detection by analyzing simulated changes in spiking activity of primary electrosensory afferents during empirically measured and synthetic prey capture trials. The animal's motor volume was reconstructed from video recordings of body motion during prey capture behavior. Our results suggest that in weakly electric fish, there is a close connection between the shape of the sensory and motor volumes. We consider three general spatial relationships between 3-D sensory and motor volumes in active and passive-sensing animals, and we examine hypotheses about these relationships in the context of the volumes we quantify for weakly electric fish. We propose that the ratio of the sensory volume to the motor volume provides insight into behavioral control strategies across all animals

The Effects of Mandatory Volunteerism on Intentions to Volunteer

With the widespread emergence of required community-service programs comes a new opportunity to examine the effects of requirements on future behavioral intentions. To investigate the consequences of such “mandatory volunteerism” programs, we followed students who were required to volunteer in order to graduate from college. Results demonstrated that stronger perceptions of external control eliminated an otherwise positive relation between prior volunteer experience and future intentions to volunteer. A second study experimentally compared mandates and choices to serve and included a premeasured assessment of whether students felt external control was necessary to get them to volunteer. After being required or choosing to serve, students reported their future intentions. Students who initially felt it unlikely that they would freely volunteer had significantly lower intentions after being required to serve than after being given a choice. Those who initially felt more likely to freely volunteer were relatively unaffected by a mandate to serve as compared with a choice. Theoretical and practical implications for understanding the effects of requirements and constraints on intentions and behavior are discussed

Genetic islands of Streptococcus agalactiae strains NEM316 and 2603VR and their presence in other Group B Streptococcal strains

BACKGROUND: Streptococcus agalactiae (Group B Streptococcus; GBS) is a major contributor to obstetric and neonatal bacterial sepsis. Serotype III strains cause the majority of late-onset sepsis and meningitis in babies, and thus appear to have an enhanced invasive capacity compared with the other serotypes that cause disease predominantly in immunocompromised pregnant women. We compared the serotype III and V whole genome sequences, strains NEM316 and 2603VR respectively, in an attempt to identify genetic attributes of strain NEM316 that might explain the propensity of strain NEM316 to cause late-onset disease in babies. Fourteen putative pathogenicity islands were described in the strain NEM316 whole genome sequence. Using PCR- and targeted microarray- strategies, the presence of these islands were assessed in a diverse strain collection including 18 colonizing isolates from healthy pregnant women, and 13 and 8 invasive isolates from infants with early- and late-onset sepsis, respectively. RESULTS: Side-by-side comparison of the strain NEM316 and strain 2603VR genomes revealed that they are extremely similar, with the only major difference being the capsulation loci and mobile genetic elements. PCR and Comparative Genome Hybridization (CGH) were used to define the presence of each island in 39 GBS isolates. Only islands I, VI, XII, and possibly X, met criteria of a true pathogenicity island, but no significant correlation was found between the presence of any of the fourteen islands and whether the strains were invasive or colonizing. Possible associations were seen between the presence of island VI and late-onset sepsis, and island X and early-onset sepsis, which warrant further investigation. CONCLUSION: The NEM316 and 2603VR strains are remarkable in that their whole genome sequences are so similar, suggesting that the capsulation loci or other genetic differences, such as pathogenicity islands, are the main determinants of the propensity of serotype III strains to cause late-onset disease. This study supports the notion that GBS strain NEM316 has four putative pathogenicity islands, but none is absolutely necessary for disease causation, whether early- or late-onset sepsis. Mobile genetic elements are a common feature of GBS isolates, with each strain having its own peculiar burden of transposons, phages, integrases and integrated plasmids. The majority of these are unlikely to influence the disease capacity of an isolate. Serotype associated disease phenotypes may thus be solely related to differences in the capsulation loci

Study of glass formation and crystallization of Y2O3-BaO-CuO-B2O3 glass-ceramics

This paper presents a series of investigations establishing the glass-forming limits from binary to quaternary in the Y2O3-BaO-CuO-B2O3 system. The glass-forming ability and crystallization behavior of the Y2O3-BaO-CuO-B2O3 system was studied by XRD, SEM and DTA techniques. Based on the binary glass-forming limits, two stable "pseudo-saturated" base glasses, with ϑx-Tg values greater than 200K, were obtained. Several "supersaturated" YBa2Cu3O6+δ - B203 glasses were made which contain about 20 mol% B2O3. Upon heat treatment, formation of tetragonal YBa2Cu3O6+δ was observed to initiate at the surface. The progressive development of the superconducting 123 phase from the "supersaturated" glasses indicates the feasibility of obtaining superconducting materials using the glass-ceramic route

The CRIT framework for identifying cross patterns in systems biology and application to chemogenomics

Biological data is often tabular but finding statistically valid connections between entities in a sequence of tables can be problematic - for example, connecting particular entities in a drug property table to gene properties in a second table, using a third table associating genes with drugs. Here we present an approach (CRIT) to find connections such as these and show how it can be applied in a variety of genomic contexts including chemogenomics data

Summary of the Large Civil Tiltrotor (LCTR2) Engine Gearbox Study

In support of the Fundamental Aeronautics Program, Subsonic Rotary Wing Project, NASA is continuing to study the Large Civil Tiltrotor (LCTR) concept to help define/refine vehicle, system and subsystem attributes. These attributes can then be used to define performance requirements and identify new or advanced technologies to achieve an operational vehicle class. As part of this goal, NASA contracted with The Boeing Company and its subcontractor Rolls-Royce to perform an investigation of different combinations of engine and gearbox variability to achieve a maximum of 50 percent rotor tip speed reduction from hover to cruise conditions. Previous NASA studies identified the 50 percent rotor speed reduction minimized vehicle gross weight and fuel burn. The LCTR2 (LCTR-iteration 2) was the contracted study baseline for initial sizing. Rotor tip speed ratios (cruise to hover) of 100, 77, and 54 percent were analyzed for each combination of engine and gearbox speed reduction to achieve the chosen rotor tip speed ratio. Three different engine and gearbox technology levels were assumed; commercial off-the-shelf (COTS), entry-in-service (EIS) in 2025 and EIS in 2035. These technology levels were applied to determine each particular effect on vehicle gross weight and fuel burn, while other vehicle technologies were assumed constant. This report summarizes the work performed that is being put together into a comprehensive NASA contractor report. Some background on the LCTR concept and baseline vehicle will be given and then a discussion concerning the technical approach utilized. Major study assumptions and results will be presented and discussed. Finally conclusions will be drawn as well as suggestions provided for future efforts

Recoiling black holes: prospects for detection and implications of spin alignment

Supermassive black hole (BH) mergers produce powerful gravitational wave (GW) emission. Asymmetry in this emission imparts a recoil kick to the merged BH, which can eject the BH from its host galaxy altogether. Recoiling BHs could be observed as offset active galactic nuclei (AGN). Several candidates have been identified, but systematic searches have been hampered by large uncertainties regarding their observability. By extracting merging BHs and host galaxy properties from the Illustris cosmological simulations, we have developed a comprehensive model for recoiling AGN. Here, for the first time, we model the effects of BH spin alignment and recoil dynamics based on the gas-richness of host galaxies. We predict that if BH spins are not highly aligned, seeing-limited observations could resolve offset AGN, making them promising targets for all-sky surveys. For randomly-oriented spins, less than about 10 spatially-offset AGN may be detectable in HST-COSMOS, and > 10^3 could be found with Pan-STARRS, LSST, Euclid, and WFIRST. Nearly a thousand velocity-offset AGN are predicted within the SDSS footprint; the rarity of large broad-line offsets among SDSS quasars is likely due in part to selection effects but suggests that spin alignment plays a role in suppressing recoils. Nonetheless, in our most physically motivated model where alignment occurs only in gas-rich mergers, hundreds of offset AGN should be found in all-sky surveys. Our findings strongly motivate a dedicated search for recoiling AGN.Comment: 30 pages, 19 figures. Accepted to MNRAS after minor revision