Can the production of wild forest foods be sustained in timber concessions? Logging and the availability of edible caterpillars hosted by sapelli (Entandrophragma cylindricum) and tali (Erythrophleum suaveolens) trees in the Democratic Republic of Congo

Sapelli (Entandrophragma cylindricum) and tali (Erythrophleum suaveolens) are among the most important timber species harvested from Congo Basin forests. They also host edible caterpillars, Imbrasia oyemensis and Cirina forda, respectively, which are important to the nutrition and income of rural and urban populations. This study evaluated the density of these tree species within a 10 km radius around each of 4 villages and in the 2012 annual cutting areas of two timber concessions in the region of Kisangani (DRC). Sapelli and tali trees ≥20 cm dbh and their stumps were identified and measured on 21 five ha plots around each village and 20 five ha plots on each concession. Around villages and on concessions, sapelli trees occurred at densities of 0.048 ± 0.008 harvestable trees (≥80 cm dbh) ha −1 and 0.135 ± 0.019 precommercial trees ha −1. Harvestable tali trees (≥60 cm dbh) were seven times more abundant at 0.347 ± 0.032 ha −1, while pre- commercial tali trees occurred at densities of 0.329 ± 0.033 trees ha −1. Between 25% and 40% of the har- vestable sapelli trees had been logged as compared to < 3% of the harvestable tali trees. Production per tree, derived from another study, was extrapolated to estimate caterpillar yields on a half circle of 15,700 ha within 10 km of villages, using these estimates of tree densities. Depending on the village, yields were estimated as 11.6–34.5 Mg year −1 of I. oyemensis from sapelli trees, and 65.8–80.9 Mg year −1 of C. forda from tali trees, an average of 0.74–2.2 kg ha −1 year and 4.2–5.2 kg ha −1 year, fresh weight, respectively (0.23–0.68 kg ha −1 year −1 and 1.3–1.6 kg ha −1 year −1, dry weight, respectively). Harvestable trees yielded more caterpillars, providing most of the C. forda caterpillars. However, because harvestable sapelli trees oc- curred at low densities, the bulk of I. oyemensis caterpillar production would be hosted on precommercial trees. Logging practices that reject poorly formed or hollow trees and guidelines that call for high minimum diameter limits and retention of seed trees or prohibit logging on slopes or riparian zones, safeguard edible caterpillar production. Multiple resource management for multiple stakeholders would require more deliberate planning and management approaches based on negotiations with local communities and approaches like setting aside collection zones or collection trees that would be protected from logging

Addressing potential conflict using participatory mapping: Collection of forest foods from timber trees around industrial concessions in Cameroon

Extensive areas of Africa's humid tropical lowland forests have been allocated to timber concessions, but are also inhabited by villagers who obtain resources from the forest. Approximately 61% of timber species in the Congo Basin also yield locally used non-timber forest products (NTFP). Among these are fruits and oil from Moabi (Baillonella toxisperma), and edible caterpillars from Sapelli (Entandrophragma cylindricum), and Tali (Erythrophleum suaveolens). Participatory mapping was used to understand whether logging affects the availability of these food resources to men and women in villages near timber concessions in two areas of Cameroon. Mapping of collection trees and interviews of 20 male and 20 female collectors, in four villages near two logging concessions, showed that during 1-day collecting trips people walk an average of 2.7 km (± 1.42) to trees where they collect these resources. Food resources are typically collected by both men and women, but men travel further to specific trees, sometimes combining this with hunting excursions further into the forest. Only 14% of the collection trees were located within the logging concessions. However, 72% of Sapelli, 81% of Moabi, and 100% of Tali trees from which food resources were collected were larger than their legal felling diameters, meaning that logging could jeopardize the supply of foods from these species. Prioritizing food values over timber values of trees within a certain radius of villages, and excluding them from logging, would safeguard villagers' access to these food resources. Already, negotiations between villagers and concessionaires have been successful. Many of the mapped collection trees occurred in agroforestry areas delineated by the concessionaire for the use of villagers; another concessionaire had suspended felling of Moabi trees in response to requests by the villagers. However, an extensive and expanding local logging sector on community forests and other lands outside of industrial timber concessions means that conflicts are arising even within villages or settlements, between individuals who seek to obtain either a one-time financial windfall from felling a tree (usually a male interest); or ensure ongoing periodic harvests of foods (typically a priority of women, but also of local Baka pygmies)

Contribution of forest foods to dietary intake and their association with household food insecurity: a cross-sectional study in women from rural Cameroon

To determine the contribution of forest foods to dietary intake and estimate their association with household food insecurity. Cross-sectional survey conducted among 279 households. Using a 7 d recall questionnaire, information on household food consumption was collected from women and used to determine the household dietary diversity score, food variety score and forest food consumption score (FFCS). Household Food Insecurity Access Scale (HFIAS) score was determined and Spearman rank correlation was used to establish the relationship between consumption of forest foods and HFIAS score. Women’s dietary intake was estimated from two 24 h recalls. The contribution of forest foods to women’s nutrient intakes was calculated and women’s nutrient intakes were compared with estimated average nutrient requirements. Rural forest-dependent households in twelve villages in eastern and southern Cameroon. Household heads and their non-pregnant, non-lactating spouses. Forty-seven unique forest foods were identified; of these, seventeen were consumed by 98 % of respondents over the course of one week and by 17 % of women during the two 24 h recall periods. Although forest foods contributed approximately half of women’s total daily energy intake, considerably greater contributions were made to vitamin A (93 %), Na (100 %), Fe (85 %), Zn (88 %) and Ca (89 %) intakes. Despite a highly biodiverse pool of foods, most households (83 %) suffered from high food insecurity based on the HFIAS. A significant inverse correlation was observed between the HFIAS score and the FFCS (r2=−0·169, P=0·0006), demonstrating that forest foods play an important role in ensuring food security in these forest-dependent communities. Forest foods are widely consumed by forest-dependent communities. Given their rich nutrient content, they have potential to contribute to food and nutrition security

Post-intervention Status in Patients With Refractory Myasthenia Gravis Treated With Eculizumab During REGAIN and Its Open-Label Extension

OBJECTIVE: To evaluate whether eculizumab helps patients with anti-acetylcholine receptor-positive (AChR+) refractory generalized myasthenia gravis (gMG) achieve the Myasthenia Gravis Foundation of America (MGFA) post-intervention status of minimal manifestations (MM), we assessed patients' status throughout REGAIN (Safety and Efficacy of Eculizumab in AChR+ Refractory Generalized Myasthenia Gravis) and its open-label extension. METHODS: Patients who completed the REGAIN randomized controlled trial and continued into the open-label extension were included in this tertiary endpoint analysis. Patients were assessed for the MGFA post-intervention status of improved, unchanged, worse, MM, and pharmacologic remission at defined time points during REGAIN and through week 130 of the open-label study. RESULTS: A total of 117 patients completed REGAIN and continued into the open-label study (eculizumab/eculizumab: 56; placebo/eculizumab: 61). At week 26 of REGAIN, more eculizumab-treated patients than placebo-treated patients achieved a status of improved (60.7% vs 41.7%) or MM (25.0% vs 13.3%; common OR: 2.3; 95% CI: 1.1-4.5). After 130 weeks of eculizumab treatment, 88.0% of patients achieved improved status and 57.3% of patients achieved MM status. The safety profile of eculizumab was consistent with its known profile and no new safety signals were detected. CONCLUSION: Eculizumab led to rapid and sustained achievement of MM in patients with AChR+ refractory gMG. These findings support the use of eculizumab in this previously difficult-to-treat patient population. CLINICALTRIALSGOV IDENTIFIER: REGAIN, NCT01997229; REGAIN open-label extension, NCT02301624. CLASSIFICATION OF EVIDENCE: This study provides Class II evidence that, after 26 weeks of eculizumab treatment, 25.0% of adults with AChR+ refractory gMG achieved MM, compared with 13.3% who received placebo

31st Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2016) : part two

Background The immunological escape of tumors represents one of the main ob- stacles to the treatment of malignancies. The blockade of PD-1 or CTLA-4 receptors represented a milestone in the history of immunotherapy. However, immune checkpoint inhibitors seem to be effective in specific cohorts of patients. It has been proposed that their efficacy relies on the presence of an immunological response. Thus, we hypothesized that disruption of the PD-L1/PD-1 axis would synergize with our oncolytic vaccine platform PeptiCRAd. Methods We used murine B16OVA in vivo tumor models and flow cytometry analysis to investigate the immunological background. Results First, we found that high-burden B16OVA tumors were refractory to combination immunotherapy. However, with a more aggressive schedule, tumors with a lower burden were more susceptible to the combination of PeptiCRAd and PD-L1 blockade. The therapy signifi- cantly increased the median survival of mice (Fig. 7). Interestingly, the reduced growth of contralaterally injected B16F10 cells sug- gested the presence of a long lasting immunological memory also against non-targeted antigens. Concerning the functional state of tumor infiltrating lymphocytes (TILs), we found that all the immune therapies would enhance the percentage of activated (PD-1pos TIM- 3neg) T lymphocytes and reduce the amount of exhausted (PD-1pos TIM-3pos) cells compared to placebo. As expected, we found that PeptiCRAd monotherapy could increase the number of antigen spe- cific CD8+ T cells compared to other treatments. However, only the combination with PD-L1 blockade could significantly increase the ra- tio between activated and exhausted pentamer positive cells (p= 0.0058), suggesting that by disrupting the PD-1/PD-L1 axis we could decrease the amount of dysfunctional antigen specific T cells. We ob- served that the anatomical location deeply influenced the state of CD4+ and CD8+ T lymphocytes. In fact, TIM-3 expression was in- creased by 2 fold on TILs compared to splenic and lymphoid T cells. In the CD8+ compartment, the expression of PD-1 on the surface seemed to be restricted to the tumor micro-environment, while CD4 + T cells had a high expression of PD-1 also in lymphoid organs. Interestingly, we found that the levels of PD-1 were significantly higher on CD8+ T cells than on CD4+ T cells into the tumor micro- environment (p < 0.0001). Conclusions In conclusion, we demonstrated that the efficacy of immune check- point inhibitors might be strongly enhanced by their combination with cancer vaccines. PeptiCRAd was able to increase the number of antigen-specific T cells and PD-L1 blockade prevented their exhaus- tion, resulting in long-lasting immunological memory and increased median survival

Constraints and opportunities for better silvicultural practice in tropical forestry: an interdisciplinary approach

Barriers to successful adoption of novel silvicultural practices are rarely just technical in nature. Simply put, why do some forest users practice better silviculture than others? Diverse perspectives in the social sciences have been brought to bear on this question, but most efforts suffer from theoretical or methodological biases which undermine their utility for answering questions of interest to forest managers and policy-makers. The authors argue that research on silviculture practice can better serve the needs of policy-makers if it is approached more holistically and with the intention of answering clear questions about why particular users have, or have not adopted desired practices in particular situations. To illustrate this approach, this paper presents three case studies of research on tropical silviculture practice from each of Philippines, Brazilian Amazon and Mexico. Findings from these studies indicate that a variety of factors may influence whether or not silvicultural practices are adopted. These range from characteristics of the local environment and individual users (knowledge, motivation, etc.) to wider geographical, economic and political influences. Forest researchers and policy-makers will better identify key constraints and opportunities for the adoption of silvicultural practices in particular contexts if they approach research with clear questions and an interdisciplinary approach