Cervical cancer, human papillomavirus (HPV), and HPV vaccination: Exploring gendered perspectives, knowledge, attitudes, and cultural taboos among Mexican American adults

Background: Gendered perspectives may be particularly important in shaping norms and values around HPV and HPV vaccination, as previous research suggests that sexuality taboos (e.g. promiscuity) may contribute to low perceived risk among adolescent and young adult Hispanic females. However, research to date focuses primarily on Hispanic mothers, adolescent females, and women of HPV vaccine-eligible age. Hispanic father\u27s perspectives are relatively unknown despite father\u27s important role in shaping norms for their female children. Objective: To close this gap, this study examines gendered perspectives in knowledge, beliefs, and attitudes about HPV and HPV vaccination from Hispanic parents (mothers and fathers), women of vaccine-eligible age (18-26 years old), and women eligible for Pap Test screening (\u3e26 years old) living in two counties along the Texas-Mexico border. Design: We conducted eight focus groups. Research staff transcribed audio recordings verbatim and uploaded them into Atlas(ti) 5.0 for analysis. The research team analyzed the data for content, meaning, patterns and themes using the constant comparison approach. Results: Perspectives were highly gendered. Women\u27s (all groups combined) beliefs focused on misconceptions around how the HPV virus is contracted (e.g. toilet surfaces). Women also linked HPV-related sexual risk to adultery and indiscretion of male partners. Fathers (men) were more likely to link risk to female promiscuity. Fathers also worried that HPV vaccination might increase promiscuity. All groups believe that HPV vaccination is a way to protect Hispanic females in the face of beliefs around sexual behavior and risk of contracting HPV. Conclusion: Results suggest gendered differences in risk beliefs concerning HPV among Hispanics living along the Texas-Mexico border. Researchers can use these findings to address barriers to HPV vaccination, as well as to create culturally appropriate prevention messages that may help reduce disparities in HPV among Hispanic women

Transport Phenomena in Alcator C-Mod H-Modes

Abstract. Several interesting new results have come from studies of ICRF-heated, H-mode plasmas in Alcator C-Mod. Dimensionless scaling studies have found gyro-Bohm-like transport similar to that reported on other devices; however, the dependence on collisionality was surprisingly strong, with BE −1. Despite high edge temperatures and strong edge pressure gradients, type I edge-localized modes (ELMs) are not observed in C-Mod. Instead we obtain a regime that we have dubbed enhanced D (EDA) which is accompanied by high-frequency density fluctuations. For all H-modes, core gradients were found to increase linearly with edge temperature, suggesting the importance of critical gradient/marginal stability behaviour. Comparisons with the IFS-PPPL model have begun, showing quantitative agreement in some cases. Impurity particle transport was studied via the laser blow-off technique with impurity confinement found to be effectively infinite for ELM-free discharges but reduced into the range 0.1–0.2 s for the EDA plasmas. 1

Active excitation and damping rate measurement of intermediate-n Toroidal Alfvén Eigenmodes in JET, C-Mod and MAST plasmas

The stability properties of Alfvén Eigenmodes (AEs) are investigated directly using external antenna excitation and detection of stable modes in a variety of plasma configurations in different devices. Dedicated methods to measure the AE damping rate separately from the fast ion drive have been pioneered at JET, using low toroidal mode number internal saddle coil antennas. Other experiments have since installed localised in-vessel antennas to drive and detect MHD modes in the Alfvén frequency range, first on C-Mod, then on MAST. Experiments on C-Mod proved for the first time that intermediate-n TAEs can be driven and detected, and point out significant differences with respect to the low-n regime on JET in the values and scaling of the damping rate with plasma parameters, e.g. the edge shape. On JET, a new antenna system, comprising two assemblies of four toroidally spaced coils each, was developed to replace the low-n saddle coil structure and excite AE modes in the toroidal mode number range that is expected to be most unstable in ITER, with n up to ~10. Experiments with the new JET antennas confirm that excitation is possible in a large volume plasma, together with real time tracking of core modes throughout the limiter and divertor phases of high performance discharges, with significant additional heating. The similarities and differences between the active MHD antenna systems, as well as a comparison of the results on C-Mod, JET and MAST are illustrated. Both C-Mod and JET results underline the fact that a precise reconstruction of the mode structure and its spectrum, important for a quantitative comparison with theoretical models, represents a significant challenge in the intermediate-n range and in the presence of several modes

APOL1 Kidney-Risk Variants Induce Mitochondrial Fission

IntroductionAPOL1 G1 and G2 nephropathy-risk variants cause mitochondrial dysfunction and contribute to kidney disease. Analyses were performed to determine the genetic regulation of APOL1 and elucidate potential mechanisms in APOL1-nephropathy.MethodsA global gene expression analysis was performed in human primary renal tubule cell lines derived from 50 African American individuals. Follow-up gene knock out, cell-based rescue, and microscopy experiments were performed.ResultsAPOL1 genotypes did not alter APOL1 expression levels in the global gene expression analysis. Expression quantitative trait locus (eQTL) analysis in polyinosinic-polycytidylic acid (poly IC)-stimulated renal tubule cells revealed that single nucleotide polymorphism (SNP) rs513349 adjacent to BAK1 was a trans eQTL for APOL1 and a cis eQTL for BAK1; APOL1 and BAK1 were co-expressed in cells. BAK1 knockout in a human podocyte cell line resulted in diminished APOL1 protein, supporting a pivotal effect for BAK1 on APOL1 expression. Because BAK1 is involved in mitochondrial dynamics, mitochondrial morphology was examined in primary renal tubule cells and HEK293 Tet-on cells of various APOL1 genotypes. Mitochondria in APOL1 wild-type (G0G0) tubule cells maintained elongated morphology when stimulated by low-dose poly IC, whereas those with G1G1, G2G2, and G1G2 genotypes appeared to fragment. HEK293 Tet-on cells overexpressing APOL1 G0, G1, and G2 were created; G0 cells appeared to promote mitochondrial fusion, whereas G1 and G2 induced mitochondrial fission. The mitochondrial dynamic regulator Mdivi-1 significantly preserved cell viability and mitochondrial cristae structure and reversed mitochondrial fission induced by overexpression of G1 and G2.ConclusionResults suggest the mitochondrial fusion/fission pathway may be a therapeutic target in APOL1-nephropathy