57 research outputs found
Avoiding degenerate coframes in an affine gauge approach to quantum gravity
In quantum models of gravity, it is surmized that configurations with
degenerate coframes could occur during topology change of the underlying
spacetime structure. However, the coframe is not the true Yang--Mills type
gauge field of the translations, since it lacks the inhomogeneous gradient term
in the gauge transformations. By explicitly restoring this ``hidden" piece
within the framework of the affine gauge approach to gravity, one can avoid the
metric or coframe degeneracy which would otherwise interfere with the
integrations within the path integral. This is an important advantage for
quantization.Comment: 14 pages, Preprint Cologne-thp-1993-H
Evidence for a novel Kit adhesion domain mediating human mast cell adhesion to structural airway cells
Background: Human lung mast cells (HLMCs) infiltrate the airway epithelium and airway smooth muscle (ASM) in asthmatic airways. The mechanism of HLMC adhesion to both cell types is only partly defined, and adhesion is not inhibited by function-blocking anti-Kit and anti-stem cell factor (SCF) antibodies. Our aim was to identify adhesion molecules expressed by human mast cells that mediate adhesion to human ASM cells (HASMCs) and human airway epithelial cells.
Methods: We used phage-display to isolate single chain Fv (scFv) antibodies with adhesion-blocking properties from rabbits immunised with HLMC and HMC-1 membrane proteins.
Results: Post-immune rabbit serum labelled HLMCs in flow cytometry and inhibited their adhesion to human BEAS-2B epithelial cells. Mast cell-specific scFvs were identified which labelled mast cells but not Jurkat cells by flow cytometry. Of these, one scFv (A1) consistently inhibited mast cell adhesion to HASMCs and BEAS-2B epithelial cells by about 30 %. A1 immunoprecipitated Kit (CD117) from HMC-1 lysates and bound to a human Kit-expressing mouse mast cell line, but did not interfere with SCF-dependent Kit signalling.
Conclusion: Kit contributes to human mast cell adhesion to human airway epithelial cells and HASMCs, but may utilise a previously unidentified adhesion domain that lies outside the SCF binding site. Targeting this adhesion pathway might offer a novel approach for the inhibition of mast cell interactions with structural airway cells, without detrimental effects on Kit signalling in other tissues
Shift of Bacterial Community in Synanthropic Mite Tyrophagus putrescentiae Induced by Fusarium Fungal Diet
Response to Weiss MF re: “Seroprevalence of Borrelia IgM and IgG Antibodies in Healthy Individuals: A Caution Against Serology Misinterpretations and Unnecessary Antibiotic Treatments”
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