3,314 research outputs found
Identification of non-host resistance genes in wheat to barley yellow rust
Yellow rust, caused by Puccinia striiformis West., is an important foliar disease of wheat and barley throughout the world, and the development of resistant cultivars is the most economical and environmentally friendly method of control. Breeding for resistance to yellow rust has, for decades, been based on the use of race-specific resistance genes, which have shown to be short-lived. Non-host resistance has been studied as a possible source of durable resistance.
Two major genes, as well as an undetermined number of minor genes, for non-host resistance to the barley attacking form of yellow rust, P. striiformis f. sp. hordei, have been previously detected in the wheat cultivar ‘Lemhi’. The present study aimed at quantifying and mapping those genes using QTL (quantitative trait loci) mapping procedures. For that purpose, an F2 population of 114 individuals resulting from the cross of resistant ‘Lemhi’ with ‘Chinese 166’, a wheat cultivar susceptible to barley yellow rust, was used as the mapping population. QTL effects and significance were estimated by means of interval mapping and MQM mapping procedures.
A map for the F2 population was constructed which included 116 DNA markers (14 SSRs and 102 AFLPs). Two major QTLs have been mapped to chromosome arms 1DS (Psh1) and 2BL (Psh2), with significant LOD values. These two QTLs account for 76.7% of the phenotypic variance for resistance to barley yellow rust. Two other QTLs, with a minor effect, were mapped to chromosome arms 5AL (Psh3) and 6AL (Psh4), explaining 5.1% and 10.9% of the phenotypic variation, respectively. The QTL on 5A was derived from the susceptible variety, ‘Chinese 166’. In all cases the resistance towards P. striiformis f.sp. hordei was associated with a visual chlorosis/necrosis response typical of race-specific, host resistance
Submillisecond monaural coincidence detection by octopus cells
In vitro and in silico studies have suggested that octopus cells in the mammalian posterior ventral cochlear nucleus (PVCN) are monaural coincidence detectors that encode the temporal structure of complex sounds. In vivo studies on these neurons, however, are rare due to several technical difficulties. We used sharp high-impedance electrodes in anesthetized gerbils to study the responses of octopus cells to click trains. We find that, even though octopus cells only fire an onset spike to pure tones, they fire in sustained fashion to trains of transients. They entrain to click trains up to 400 Hz with vector strength almost equal to one and spike jitter at similar to 100 microseconds. This temporal precision is unmatched by any other cell type in the auditory system
Magnetic-field dependence of electron spin relaxation in n-type semiconductors
We present a theoretical investigation of the magnetic field dependence of
the longitudinal () and transverse () spin relaxation times of
conduction band electrons in n-type III-V semiconductors. In particular, we
find that the interplay between the Dyakonov-Perel process and an additional
spin relaxation channel, which originates from the electron wave vector
dependence of the electron -factor, yields a maximal at a finite
magnetic field. We compare our results with existing experimental data on
n-type GaAs and make specific additional predictions for the magnetic field
dependence of electron spin lifetimes.Comment: accepted for publication in PRB, minor changes to previous manuscrip
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Identification of host odour attractants for tsetse flies. Final report 1986-1990
Tsetse flies, Glossina spp., are blood-feeding insects and vectors of trypanosomes, microorganisms which cause sleeping sickness in man and a similar disease, "nagana" in domestic animals. The economic importance of trypanosomiasis is the constraint it imposes on orderly rural development in Africa, leading to under-exploitation of infested land and over-exploitation and degradation of trypanosomiasis-free areas.
Traps and targets which attract tsetse flies and kill them could provide environmentally-acceptable, appropriate technology for monitoring and control of tsetse in Africa. Unbaited devices providing only visual attraction have proved effective in monitoring and control of riverine species of tsetse, but not the savannah species found in the fly belt of Malawi, Mozambique, Zambia and Zimbabwe covered by the EDF Regional Tsetse and Trypanosomiasis Control Project (RTTCP).
Previously, collaborative was begun between glossinologists of the Zimbabwe Department of Veterinary Services (DVS) and UK Tsetse Research Laboratory (TRL) and chemists at NRI. This brought together the experience of the DVS in the field, the experience of TRL in laboratory bioassay work, and the experience of NRI in using gas chromatography linked to electroantennography (GC-EAG) and chemical techniques to detect and identify insect behaviour-modifying chemicals. Tsetse attractants produced by host animals were identified and synthesised, and dispensing systems for these compounds devised. Traps and targets impregnated with insecticide, baited with these lures were shown to provide effective control of the savannah tsetse species, G. pallidipes and G. m. morsitans
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