Immune Changes in the Anterior Kidney of Spawning Sockeye Salmon

During the return journey to their spawning grounds, sockeye salmon are exposed to various pathogens and undergo major endocrine changes. Little is known about how these changes affect their immune system. The immune system of salmon is similar to mammals; myeloid lineage cells provide the first response to infection and B lineage cells protect against specific pathogens. After activation by pathogen, B cells may differentiate into long-lived plasma cells (LLPCs) in the anterior kidney, where they can survive for years, continuously secreting protective antibody. This research focused on salmon from two rivers, the Kenai and the Copper River, and characterized changes in anterior kidney immune cell abundance during their journey and at various spawning sites. Additionally, possible correlations with relative spleen size, distance traveled, water temperature, and infections with Infectious Hematopoietic Necrosis Virus were investigated. Flow cytometry was used to analyze the abundance of B cells, LLPCs, and myeloid lineage cells in the anterior kidney. Quantitative PCR was used to determine IHNV load in the spleen. During the Kenai River Run, myeloid cells and activated B cells transiently decreased while (im)mature B cells, IgM+ B cells, and LLPCs transiently increased. The changes in myeloid cells, IgM+ B cells, and LLPCs were conserved at the Kenai and Copper River Runs. Analysis indicated that a larger spleen index correlated with immune system activation and a lower prevalence of IHNV. These findings provide a better understanding of immune changes during spawning in sockeye salmon

Emergence of Ixodes scapularis and Borrelia burgdorferi, the Lyme disease vector and agent, in Ohio

Lyme disease, the most common vector-borne disease in the United States, is caused by a tick-borne infection with Borrelia burgdorferi. Currently, Ohio is considered by the Centers for Disease Control and Prevention (CDC) to be non-endemic for Lyme disease. The low incidence of Lyme disease in this state was largely attributed to the absence of the transmitting vector, Ixodes scapularis, commonly known as the blacklegged tick. However, a tick surveillance program established by Ohio Department of Health indicated that the number of I. scapularis ticks in Ohio had increased sharply in recent years, from 0 - 5 ticks per year during 1983 - 2008 to 15 in 2009, 40 in 2010, and 184 in 2011. During the fall deer hunting season, examination of deer heads submitted to Ohio Department of Agriculture found 29 I. scapularis from 7 counties in 2010 and 1,830 from 25 counties in 2011. As of 2012, the tick had been found in 57 of the 88 counties of Ohio. In addition, all three active stages (larva, nymph, and adult) of I. scapularis were found in Tiverton Township of Coshocton County, demonstrating the presence of established tick populations at this central Ohio location. Of 530 nymphal or adult I. scapularis analyzed by quantitative polymerase chain reaction (qPCR), 32 (6.1%) tested positive for the B. burgdorferi flaB gene, ranging from 36 to 390,000 copies per tick. Antibodies to B. burgdorferi antigens were detected in 2 of 10 (20%) field-captured Peromyscus leucopus from Tiverton Township, and in 41 of 355 (11.5%) dogs residing in Ohio. Collectively, these data suggest that the enzootic life cycle of B. burgdorferi has become established in Ohio, which poses risk of Lyme disease to people and animals in the area

Rapid 13(c) urea breath test to identify Helicobacter pylori infection in emergency department patients with upper abdominal pain

Introduction: In emergency department (ED) patients with upper abdominal pain, management includes ruling out serious diseases and providing symptomatic relief. One of the major causes of upper abdominal pain is an ulcer caused by Helicobacter pylori (H. pylori), which can be treated and cured with antibiotics. We sought to estimate the prevalence of H. pylori infection in symptomatic patients using a convenience sample at a single urban academic ED and demonstrate the feasibility of ED-based testing. Methods: We prospectively enrolled patients with a chief complaint of pain or discomfort in the upper abdomen for 1 year from February 2011 until February 2012 at a single academic urban ED. Enrolled subjects were tested for H. pylori using a rapid point of care 13C Urea Breath Test (UBT) [Exalenz Bioscience]. We compared patient characteristics between those who tested positive versus negative for the disease. Results: A total of 205 patients with upper abdominal pain were tested over 12 months, and 24% (95% confidence interval: 19% to 30%) tested positive for H. pylori. Black subjects were more likely to test positive than white subjects (28% v. 6%, P \u3c 0.001). Other factors, such as age and sex, were not different between the 2 groups. Conclusion: In our ED, H. pylori infection was present in 1 in 4 patients with epigastric pain, and testing with a UBT was feasible. Further study is needed to determine the risk factors associated with infection, the prevalence of H. pylori in other EDs, the effect of the test on ED length of stay and the cost-effectiveness of an ED-based test-and-treat strategy. [West J Emerg Med. 2013;14(3):278–282.

Reducing PostPartum Hemorrhage Rates at Maine Medical Center

In the United States, approximately 700 women die each year from pregnancy related deaths and the most frequent cause of preventable maternal mortality is obstetric hemorrhage. The postpartum hemorrhage (PPH) rate at Maine Medical Center (MMC) is three times the national average. At our facility we care for the most complex patients in the State and we must decrease our rate to accurately reflect our expertise, knowledge and skills

A Rooted University: Growing Resiliency, Community, and Engaged Food Citizens at the UM Campus Farm

In response to the recommendation from the Campus Sustainability Integrated Assessment of University of Michigan (2009), and the long-time vision of a core group of faculty and staff representing the Sustainable Food Initiative at the University of Michigan, the School of Natural Resources and Environment’s Sustainable Food Program 2012-2013 Master’s Project Team established the University of Michigan Sustainable Food Program (UMSFP) and the University of Michigan Campus Farm. Since its inception, UMSFP has remained an entirely student-driven initiative. A leadership team of four graduate students and four undergraduate students is responsible for program oversight and makes all of the core decisions surrounding its administration. In January 2013, UMSFP Program Manager, Emily Canosa, was hired to provide mentorship and support to the student leaders and to aid in the continuity to the program, as student leadership turns over each year. Emily is the only non-student member involved in the UMSFP leadership meetings. UMSFP also has an advisory board comprised of the students on the UMSFP leadership team, faculty, and staff. The program has since blossomed into a community of 10 active member groups, each representing unique interests in sustainable food. One of these member groups, Friends of the Campus Farm, along with student interns hired by the Matthaei Botanical Gardens, are responsible for directing and managing the Campus Farm, located at the Matthaei Botanical Gardens. The Farm began as a pilot program in May 2012, and it grew to its full production space of two acres, with a quarter acre cultivated, in Summer 2013. The Campus Farm was founded with this threefold mission (Dengate et. al, 2013): 1. Develop responsible citizens and leaders by facilitating formal and informal education on sustainable food topics. 2. Strengthen communities through collaborative programming and outreach 3. Grow sustainable food that supports the well-being of people and the environmental at the University of Michigan and beyond In line with this mission, programming at the farm has flourished since the planting of the pilot plot in May 2012: • Students can buy produce on campus in the fall at MFarmers’ Markets and at UMSFP’s member group Student Food Co.’s bi-weekly produce table • University courses incorporate the farm into their curriculum and projects, racking up more than 600 hours of course contact annually • Friends of the Campus Farm schedules regular workdays and volunteer opportunities for upwards of forty loyal volunteers who donate over 1900 hours of their time to the Farm each year. • New students get acquainted with the farm during Welcome Week and orientations • Three hundred community members celebrate each year at the Harvest FestivalMaster of ScienceNatural Resources and EnvironmentUniversity of Michiganhttp://deepblue.lib.umich.edu/bitstream/2027.42/106572/1/A Rooted University.pd

Differences in Small Molecule Neurotransmitter Profiles From the Crown-of-Thorns Seastar Radial Nerve Revealed Between Sexes and Following Food-Deprivation

Neurotransmitters serve as chemical mediators of cell communication, and are known to have important roles in regulating numerous physiological and metabolic events in eumetazoans. The Crown-of-Thorns Seastar (COTS) is an asteroid echinoderm that has been the focus of numerous ecological studies due to its negative impact on coral reefs when in large numbers. Research devoted to its neural signaling, from basic anatomy to the key small neurotransmitters, would expand our current understanding of neural-driven biological processes, such as growth and reproduction, and offers a new approach to exploring the propensity for COTS population explosions and subsequent collapse. In this study we investigated the metabolomic profiles of small molecule neurotransmitters in the COTS radial nerve cord. Multivariate analysis shows differential abundance of small molecule neurotransmitters in male and female COTS, and in food-deprived individuals with significant differences between sexes in gamma-aminobutyric acid (GABA), histamine and serotonin, and significant differences in histamine and serotonin between satiation states. Annotation established that the majority of biosynthesis enzyme genes are present in the COTS genome. The spatial distribution of GABA, histamine and serotonin in the radial nerve cord was subsequently confirmed by immunolocalization; serotonin is most prominent within the ectoneural regions, including unique neural bulbs, while GABA and histamine localize primarily within neuropil fibers. Glutamic acid, which was also found in high relative abundance and is a precursor of GABA, is known as a spawning inhibitor in seastars, and as such was tested for inhibition of ovulation ex-vivo which resulted in complete inhibition of oocyte maturation and ovulation induced by 1-Methyladenine. These findings not only advance our knowledge of echinoderm neural signaling processes but also identify potential targets for developing novel approaches for COTS biocontrol

Structure and proteomic analysis of the crown-of-thorns starfish (Acanthaster sp.) radial nerve cord

The nervous system of the Asteroidea (starfish or seastar) consists of radial nerve cords (RNCs) that interconnect with a ring nerve. Despite its relative simplicity, it facilitates the movement of multiple arms and numerous tube feet, as well as regeneration of damaged limbs. Here, we investigated the RNC ultrastructure and its molecular components within the of Pacific crown-of-thorns starfish (COTS; Acanthaster sp.), a well-known coral predator that in high-density outbreaks has major ecological impacts on coral reefs. We describe the presence of an array of unique small bulbous bulbs (40–100 μm diameter) that project from the ectoneural region of the adult RNC. Each comprise large secretory-like cells and prominent cilia. In contrast, juvenile COTS and its congener Acanthaster brevispinus lack these features, both of which are non-corallivorous. Proteomic analysis of the RNC (and isolated neural bulbs) provides the first comprehensive echinoderm protein database for neural tissue, including numerous secreted proteins associated with signalling, transport and defence. The neural bulbs contained several neuropeptides (e.g., bombyxin-type, starfish myorelaxant peptide, secretogranin 7B2-like, Ap15a-like, and ApNp35) and Deleted in Malignant Brain Tumor 1-like proteins. In summary, this study provides a new insight into the novel traits of COTS, a major pest on coral reefs, and a proteomics resource that can be used to develop (bio)control strategies and understand molecular mechanisms of regeneration.journal articl

The price of persistence: Assessing the drivers and health implications of metal levels in indicator carnivores inhabiting an agriculturally fragmented landscape

Patterns and practices of agricultural expansion threaten the persistence of global biodiversity. Wildlife species surviving large-scale land use changes can be exposed to a suite of contaminants that may deleteriously impact their health. There is a paucity of data concerning the ecotoxicological impacts associated with the global palm oil (Elaeis guineensis) industry. We sampled wild Malay civets (Viverra tangalunga) across a patchwork landscape degraded by oil palm agriculture in Sabah, Malaysian Borneo. Using a non-lethal methodology, we quantified the levels of 13 essential and non-essential metals within the hair of this adaptable small carnivore. We robustly assessed the biological and environmental drivers of intrapopulation variation in measured levels. Metal concentrations were associated with civet age, weight, proximity to a tributary, and access to oxbow lakes. In a targeted case study, the hair metal profiles of 16 GPS-collared male civets with differing space use patterns were contrasted. Civets that entered oil palm plantations expressed elevated aluminium, cadmium, and lead, and lower mercury hair concentrations compared to civets that remained exclusively within the forest. Finally, we paired hair metal concentrations with 34 blood-based health markers to evaluate the possible sub-lethal physiological effects associated with varied hair metal levels. Our multi-facetted approach establishes these adaptable carnivores as indicator species within an extensively altered ecosystem, and provides critical and timely evidence for future studies

Suppression of rice methane emission by sulfate deposition in simulated acid rain

Sulfate in acid rain is known to suppress methane (CH4) emissions from natural freshwater wetlands. Here we examine the possibility that CH4 emissions from rice agriculture may be similarly affected by acid rain, a major and increasing pollution problem in Asia. Our findings suggest that acid rain rates of SO2-4 deposition may help to reduce CH4 emissions from rice agriculture. Emissions from rice plants treated with simulated acid rain at levels of SO2-4 consistent with the range of deposition in Asia were reduced by 24% during the grain filling and ripening stage of the rice season which accounts for 50% of the overall CH4 that is normally emitted in a rice season. A single application of SO2-4 at a comparable level reduced CH4 emission by 43%. We hypothesize that the reduction in CH4 emission may be due to a combination of effects. The first mechanism is that the low rates of SO2-4 may be sufficient to boost yields of rice and, in so doing, may cause a reduction in root exudates to the rhizosphere, a key substrate source for methanogenesis. Decreasing a major substrate source for methanogens is also likely to intensify competition with sulfate-reducing microorganisms for whom prior SO2-4 limitation had been lifted by the simulated acid rain S deposition

The relationship of small vessel disease burden on cerebral and regional brain atrophy rates and cognitive performance over one year of follow-up after transient ischemic attack

BackgroundStroke, even when minor, increases the risk of dementia. We aimed to determine whether patients with transient ischaemic attack (TIA) exhibit higher rates of cerebral and regional atrophy 1-year after first stroke symptoms and evaluate the relationship with small vessel disease and cognitive performance.MethodsTIA patients and controls without cognitive symptoms underwent high-resolution T1-weighted MRI and cognitive testing at baseline and 1-year. Percent brain volume change (PBVC) was measured, and the location of regional atrophy and small vessel disease (CSVD) burden was evaluated. Neuropsychological testing assessed memory, processing speed, and executive function.ResultsA total of 76 TIA patients and 53 controls of mean age 67 (SD = 8) and 68 years (SD = 8) were recruited. TIA patients demonstrated greater improvement of visual memory and executive function at 1-year. TIA patients had greater median PBVC/year compared to controls (−0.79% [(−1.22)-(−0.38)] vs. -0.41% [(−0.62)-0.19]; p < 0.001), and higher rates of volume loss (ml/year) in subcortical gray (−0.53 [(−1.09)-(−0.06)] vs. -0.13 [(−0.61)-0.31]; p < 0.05) and white matter (−2.21 [−5.47, 0.40] vs. -0.93 [(−3.43)-2.10]; p < 0.05). Linear regression showed that TIA, age, and systolic blood pressure (SBP) were associated with greater cerebral volume loss over 1-year. There was no significant relationship between PBVC and 1-year cognition.ConclusionA near two-fold increase in rate of cerebral atrophy 1-year after TIA is associated with higher SBP emphasizing the need for improved treatment of SBP. Cerebral and regional atrophy rates may be used to select patients for vascular risk reduction trials or novel therapeutics in future dementia prevention trials