Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Increasing protein distribution has no effect on changes in lean mass during a rugby preseason

Increasing the frequency of protein consumption is recommended to stimulate muscle hypertrophy with resistance exercise. This study manipulated dietary protein distribution to assess the effect on gains in lean mass during a rugby preseason. Twenty-four developing elite rugby athletes (age 20.1 ± 1.4 years, mass 101.6 ± 12.0 kg; M ± SD) were instructed to consume high biological value (HBV) protein at their main meals and immediately after resistance exercise while limiting protein intake between meals. To manipulate protein intake frequency, the athletes consumed 3 HBV liquid protein supplements (22 g protein) either with main meals (bolus condition) or between meals (frequent condition) for 6 weeks in a 2 x 2 crossover design. Dietary intake and change in lean mass values were compared between conditions by analysis of covariance and correlational analysis. The dietary manipulation successfully altered the protein distribution score (average number of eating occasions containing > 20 g of protein) to 4.0 ± 0.8 and 5.9 ± 0.7 (p < .01) for the bolus and frequent conditions, respectively. There was no difference in gains in lean mass between the bolus (1.4 ± 1.5 kg) and frequent (1.5 ± 1.4 kg) conditions (p = .91). There was no clear effect of increasing protein distribution from approximately 4-6 eating occasions on changes in lean mass during a rugby preseason. However, other dietary factors may have augmented adaptation. © 2016 Human Kinetics, Inc

The effect of a whey protein supplement dose on satiety and food intake in resistance training athletes

Objective: Many athletes perform resistance training and consume dietary protein as a strategy to promote anabolic adaptation. Due to its high satiety value, the regular addition of supplemented dietary protein could plausibly displace other key macronutrients such as carbohydrate in an athlete's diet. This effect will be influenced by the form and dose of protein. Therefore, this study assessed the impact of liquid whey protein dose manipulation on subjective sensations of appetite and food intake in a cohort of athletes. Design: Ten male athletes who performed both resistance and aerobic (endurance) training (21.2 ± 2.3 years; 181.7 ± 5.7 cm and 80.8 ± 6.1 kg) were recruited. In four counter-balanced testing sessions they consumed a manipulated whey protein supplement (20, 40, 60 or 80 g protein) 1 hour after a standardised breakfast. Subsequent energy intake was measured 3 hours after the protein supplement using an ad libitum test meal. Subjective appetite sensations were measured periodically during the test day using visual analogue scales. Results: All conditions resulted in a significant decrease in ratings of hunger (50–65%; P < 0.05) at the time of supplement consumption. However, there were no significant differences between the conditions at any time point for subjective appetite sensations or for energy consumed in the ad libitum meal: 4382 ± 1004, 4643 ± 982, 4514 ± 1112, 4177 ± 1494 kJ respectively. Conclusion: Increasing whey protein supplement dose above 20 g did not result in a measurable increase in satiety or decrease in food intake. However, the inclusion of additional whey protein supplementation where not otherwise consumed could plausibly reduce dietary intake

Are increases in skeletal muscle mass accompanied by changes to resting metabolic rate in rugby athletes over a pre-season training period?

Optimising dietary energy intake is essential for effective sports nutrition practice in rugby athletes. Effective dietary energy prescription requires careful consideration of athletes’ daily energy expenditure with the accurate prediction of resting metabolic rate (RMR) important due to its influence on total energy expenditure and in turn, energy balance. This study aimed to (a) measure rugby athletes RMR and (b) report the change in RMR in developing elite rugby players over a rugby preseason subsequent to changes in body composition and (c) explore the accurate prediction of RMR in rugby athletes. Eighteen developing elite rugby union athletes (age 20.2 ± 1.7 years, body mass 101.2 ± 14.5 kg, stature 184.0 ± 8.4 cm) had RMR (indirect calorimetry) and body composition (dual energy x-ray absorptiometry) measured at the start and end of a rugby preseason ∼14 weeks later. There was no statistically significant difference in RMR over the preseason period (baseline 2389 ± 263 kcal·day−1 post 2373 ± 270 kcal·day−1) despite a significant increase in lean mass of +2.0 ± 1.6 kg (P < 0.01) and non-significant loss of fat mass. The change in RMR was non-significant and non-meaningful; thus, this study contradicts the commonly held anecdotal perception that an increase in skeletal muscle mass will result in a significant increase in metabolic rate and daily energy needs. Conventional prediction equations generally under-estimated rugby athletes’ measured RMR, and may be problematic for identifying low energy availability, and thus updated population-specific prediction equations may be warranted to inform practice

Relationship Between Gait Mechanics and the Speed of the Trot in the Weimaraner Dog Breed

While the size of the Weimaraner may assist in the breed performing the tasks of a sporting dog, the large size coupled with these tasks may also make the breed more susceptible to orthopedic issues. The understanding of the normal gait mechanics of the Weimaraner can be a useful tool in examining for gait abnormalities associated with these orthopedic issues, and yet, research concerning breed-specific gaits in the canine is limited. Therefore, study objectives were to define the normal Weimaraner trotting kinematics and determine the influence of speed on these parameters. Markers were attached to palpation points on the limbs and head of American Kennel Club registered Weimaraners. Dogs were tracked while performing a slow (1.2-1.7 m/s) and fast (1.9-2.3 m/s) trotting speed. Frame-by-frame analysis was performed. Paw ground contact and lift-off was documented and marker displacement was tracked. At both speeds, the trot had a diagonal footfall sequence with diagonal limb pairing alternating between diagonal bipedal support and suspension. The faster speed was achieved with significant increases in stride length and displacements of the head, withers, and fore and hind paws (P \u3c.05). Range of motion of the elbow and hip significantly increased as the dog transitioned from a slow to fast speed (P \u3c.05). Through gait analysis, the Weimaraner trot was defined as a 2-beat diagonal rhythm gait with suspension. Speed did not change these characteristics, but did influence stride length and linear and angular displacements, and thus, should be a consideration in clinical examination

Sound support: intermodal information facilitates infants’ perception of an occluded trajectory

In a visual occlusion task, 4-month-olds were given a dynamic sound cue (following the trajectory of an object), or a static cue (sound remained stationary). Infants’ oculomotor anticipations were greater in the Dynamic condition, suggesting that representations of visual occlusion were supported by auditory information

Winner determination in voting trees with incomplete preferences and weighted votes

In multiagent settings where agents have different preferences, preference aggregation can be an important issue. Voting is a general method to aggregate preferences. We consider the use of voting tree rules to aggregate agents ’ preferences. In a voting tree, decisions are taken by performing a sequence of pairwise comparisons in a binary tree where each comparison is a majority vote among the agents. Incompleteness in the agents ’ preferences is common in many real-life settings due to privacy issues or an ongoing elicitation process. We study how to determine the winners when preferences may be incomplete, not only for voting tree rules (where the tree is assumed to be fixed), but also for the Schwartz rule (in which the winners are the candidates winning for at least one voting tree). In addition, we study how to determine the winners when only balanced trees are allowed. In each setting, we address the complexity of computing necessary (respectively, possible) winners, which are those candidates winning for all completions (respectively, at least one completion) of the incomplete profile. We show that many such winner determination problems are computationally intractable when the votes are weighted. However, in some cases, the exact complexity remains unknown. Since it is generally computationally difficult to find the exact set of winners for voting trees and the Schwartz rule, we propose several heuristics that find in polynomial time a superset of the possible winners and a subset of the necessary winners which are based on the completions of the (incomplete) majority graph built from the incomplete profiles.

Toddlers with elevated Autism symptoms show slowed habituation to faces

We explored social information processing and its relation to social and communicative symptoms in toddlers with Autism Spectrum Disorder (ASD) and their siblings. Toddlers with more severe symptoms of autism showed slower habituation to faces than comparison groups; slower face learning correlated with poorer social skills and lower verbal ability. Unaffected toddlers who were siblings of children with ASD also showed slower habituation to faces compared with toddlers without siblings with ASD. We conclude that slower rates of face learning may be an endophenotype of ASD and is associated with more severe symptoms among affected individuals