168 research outputs found
Differential protein expression during growth on model and commercial mixtures of naphthenic acids in Pseudomonas fluorescens Pf‐5
Naphthenic acids (NAs) are carboxylic acids with the formula (CnH2n+ZO2) and are among the most toxic, persistent constituents of oil sands process-affected waters (OSPW), produced during oil sands extraction. Currently, the proteins and mechanisms involved in NA biodegradation are unknown. Using LC-MS/MS shotgun proteomics, we identified proteins overexpressed during the growth of Pseudomonas fluorescens Pf-5 on a model NA (4′-n-butylphenyl)-4-butanoic acid (n-BPBA) and commercial NA mixture (Acros). By day 11, >95% of n-BPBA was degraded. With Acros, a 17% reduction in intensity occurred with 10–18 carbon compounds of the Z family −2 to −14 (major NA species in this mixture). A total of 554 proteins (n-BPBA) and 631 proteins (Acros) were overexpressed during growth on NAs, including several transporters (e.g., ABC transporters), suggesting a cellular protective response from NA toxicity. Several proteins associated with fatty acid, lipid, and amino acid metabolism were also overexpressed, including acyl-CoA dehydrogenase and acyl-CoA thioesterase II, which catalyze part of the fatty acid beta-oxidation pathway. Indeed, multiple enzymes involved in the fatty acid oxidation pathway were upregulated. Given the presumed structural similarity between alkyl-carboxylic acid side chains and fatty acids, we postulate that P. fluorescens Pf-5 was using existing fatty acid catabolic pathways (among others) during NA degradation
Examining the management of stake and interest in a participatory design Facebook group
This paper analyses the micro-dynamics of a participatory design (PD) Facebook group for breast screening. We argue that using online PD methods enables participants to be fully involved throughout the research process and can lead to meaningful outcomes and impact for the research. It is important to ensure that all stakeholders are equally involved in the research. As such, understanding how a user’s stake or interest is managed can help to uncover how professionals and lay people participate together in such groups. A case study approach is adopted, with an example presented from a PD Facebook group established to develop a web resource around breast screening. 70 threads from the Facebook group were analysed in a naturalistic way using discursive psychology. The analysis shows how participants aimed to manage the dilemma of being seen as interested due to their professional identities through referring to joint membership of the design group. However, there were still challenges in ensuring that lay members’ contributions were not diminished by more professional members. The analysis suggests that in an online PD group, moderators may be needed to ensure that potential conflicts of interest are managed appropriately and all members’ views are heard
Is drinking water a risk factor for endemic cryptosporidiosis? A case-control study in the immunocompetent general population of the San Francisco Bay Area
BACKGROUND: Cryptosporidiosis, caused by Cryptosporidium, is an enteric illness that has received much attention as an infection of immunocompromised persons as well as in community outbreaks (frequently waterborne). There are, however, no studies of the risk factors for sporadic community-acquired cryptosporidiosis in the immunocompetent US population. We undertook a case-control study in the San Francisco Bay Area as part of a national study sponsored by the Centers for Disease Control and Prevention to ascertain the major routes of transmission for endemic cryptosporidiosis, with an emphasis on evaluating risk from drinking water. METHODS: Cases were recruited from a population-based, active surveillance system and age-matched controls were recruited using sequential random-digit dialing. Cases (n = 26) and controls (n = 62) were interviewed by telephone using a standardized questionnaire that included information about the following exposures: drinking water, recreational water, food items, travel, animal contact, and person-to-person fecal contact, and (for adults) sexual practices. RESULTS: In multivariate conditional logistic regression analyses no significant association with drinking water was detected. The major risk factor for cryptosporidiosis in the San Francisco Bay Area was travel to another country (matched odds ratio [95% confidence interval]: 24.1 [2.6, 220]). CONCLUSION: The results of this study do not support the hypothesis that drinking water is an independent risk factor for cryptosporidiosis among the immunocompetent population. These findings should be used to design larger studies of endemic cryptosporidiosis to elucidate the precise mechanisms of transmission, whether waterborne or other
A framework to assess quality and uncertainty in disaster loss data
There is a growing interest in the systematic and consistent collection of disasterloss data for different applications. Therefore, the collected data must follow a set oftechnical requirements to guarantee its usefulness. One of those requirements is theavailability of a measure of the uncertainty in the collected data to express its quality for agiven purpose. Many of the existing disaster loss databases do not provide such uncertainty/qualitymeasures due to the lack of a simple and consistent approach to expressuncertainty. After reviewing existing literature on the subject, a framework to express theuncertainty in disaster loss data is proposed. This framework builds on an existinguncertainty classification that was updated and combined with an existing method for datacharacterization. The proposed approach is able to establish a global score that reflects theoverall uncertainty in a certain loss indicator and provides a measure of its quality
Robust Estimation of Wage Dispersion with Censored Data: An Application to Occupational Earnings Risk and Risk Attitudes
We present a semiparametric method to estimate group-level dispersion, which is particularly effective in the presence of censored data. We apply this procedure to obtain measures of occupation-specific wage dispersion using top-coded administrative wage data from the German IAB Employment Sample (IABS). We then relate these robust measures of earnings risk to the risk attitudes of individuals working in these occupations. We find that willingness to take risk is positively correlated with the wage dispersion of an individual's occupation
The mastodons and mammoths of Michigan /
Master of ScienceGeologyUniversity of Michiganhttp://deepblue.lib.umich.edu/bitstream/2027.42/115109/1/39015003271783.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/115109/2/39015003271783.pd
Participation of adolescents in protection: dividends for all
In order to keep children and adolescents safe, and improve their chances of living fulfilling lives, we need to listen and respond to their views and opinions on matters that affect them
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Global variation in the relationship between avian phylogenetic diversity and functional distance is driven by environmental context and constraints
Funder: Gates Cambridge Trust; doi: http://dx.doi.org/10.13039/501100005370AbstractAimIf evolutionary distance is akin to evolutionary chance, then it follows that species assemblages that are distantly related will also be more disparate in terms of their traits, features and the niches they occupy. Yet, studies have found that the total phylogenetic distance of an assemblage, known as phylogenetic diversity, is an unreliable surrogate for functional diversity. We investigate global variation in the relationship between Faith's phylogenetic diversity (PD) and mean pairwise functional distance (MPFD) across latitude and the influence of migratory species on both these aspects of diversity.LocationGlobal.Time periodPresent day.Major taxa studiedBirds.MethodsWe measure PD and MPFD for over 9000 species of bird across more than 17,000 globally distributed assemblages. We obtain standardised effect sizes for both indices by simulating assemblage composition under an ecologically informed null model. We employ path analysis to characterize variation in the relationship between PD's and MPFD across latitude, elevation and with proportion of migratory species.ResultsGlobally, assemblages that were phylogenetically diverse tended to be less functionally dispersed than expected; however, this relationship showed considerable variation across latitude decreasing with distance from the equator. The proportion of migratory species in an assemblage was found to be an important predictor of functional diversity, with migrant rich assemblages generally showing less functional diversity than expected. We identify the Andes and Hengduan Mountains as regions of exceptional bird functional diversity.Main conclusionsThe relationship between phylogenetic diversity and function diversity is context specific, varying across environmental gradients such as latitude, and influenced by ecological phenomena such as migration. Thus, care should be taken using phylogenetic diversity as a proxy for functional diversity, particularly in clades with sparse functional data. Instead, we recommend that studies consider how phylogenetic diversity's surrogacy for functional diversity may be impacted by environmental context and evaluate empirical observations against biogeographically constrained and ecologically informed null models.</jats:sec
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