USING RANDOMLY AMPLIFIED DNA FINGERPRINTING (RAF) FOR GENOTYPING COMMON AND DURUM WHEATS VARIETIES (TRITICUM DURUM AND T. AESTIVUM)

The report analyzes the approach to genotyping varieties of common and durum wheat. It is shown that using of RAF-analysis 2 separate clusters of common wheat varieties and durum wheat varieties with little overlap. However, the purity of seeds doesn’t allow us to determine the specific varieties for sure without the use of reference samples

Layer-by-layer assembled highly absorbing hundred-layer films containing a phthalocyanine dye: Fabrication and photosensibilization by thermal treatment

Highly absorbing hundred-layer films based on poly(diallyldimethylammonium chloride) (PDADMAC) of various molecular weights and on sulfonated copper phthalocyanine (CuPcTs) were prepared using layer-by-layer assembly. The multilayer films grew linearly up to 54 bilayers, indicating that the same amount of CuPcTs was adsorbed at each deposition step. This amount, however, was dependent on the molecular weight of PDADMAC in the range 100-500 kDa: the higher the molecular weight, the more CuPcTs molecules were adsorbed. This can be explained by the larger surface charge number density specific to longer polymer chains. Domains of pure PDADMAC and of the PDADMAC/CuPcTs complexwere formed in the films during the assembly. Uniform distribution of CuPcTs over the films could be achieved by thermal treatment, leading to an alpha -> beta phase transition in phthalocyanine at 300 degrees C. Annealing caused changes in the film absorbance spectra, resulting in a 30-nm red shift of the peak maxima and in a strong (up to 62%) decrease in optical density. Thermogravimetric analysis revealed thermodegradation of PDADMAC during annealing above 270 degrees C, giving rise to micrometersized cracks within the films, as evidenced by scanning electron microscopy