66 research outputs found
DNA barcoding evidence for the North American presence of alfalfa cyst nematode, Heterodera medicaginis
Specimens of Heterodera have been collected from alfalfa fields in Kearny County, Kansas and Carbon County, Montana. DNA barcoding with the COI mitochondrial gene indicate that the species is not Heterodera glycines, soybean cyst nematode, H. schachtii, sugar beet cyst nematode, or H. trifolii, clover cyst nematode. Maximum likelihood phylogenetic trees show that the alfalfa specimens form a sister clade most closely related to H. glycines, with a 4.7% mean pairwise sequence divergence across the 862 nucleotides of the COI marker. Morphological analyses of juveniles and cysts conform to the measurements of H. medicaginis, the alfalfa cyst nematode originally described from the USSR in 1971. Initial host testing demonstrated that the nematode reproduced on alfalfa, but not on soybeans, tomato, or corn. Collectively, the evidence suggests that this finding represents the first record of H. medicaginis in North America. Definitive confirmation of this diagnosis would require COI sequence of eastern European isolates of this species
Molecular and morphological characterization of the alfalfa cyst nematode, Heterodera medicaginis, from Utah
Morphological and Molecular Characterization of Two Aphelenchoides Endophytic in Poplar Leaves
A novel class of CoA-transferase involved in short-chain fatty acid metabolism in butyrate-producing human colonic bacteria
Peer reviewedPublisher PD
Detection of Rhynchophorus palmarum (Coleoptera: Curculionidae) and Identification of Associated Nematodes in South Texas
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Developing a Real-Time PCR Assay for Detection and Quantification of Pratylenchus neglectus in Soil
Pratylenchus neglectus is one of the most widespread and economically important nematodes that invades plant roots and restricts wheat productivity in the Pacific Northwest. It is challenging to quantify P neglectus using microscopic methods for studies that require large-scale sampling, such as assessment of rotation crops, wheat cultivars, and other management practices. A real-time quantitative polymerase chain reaction (qPCR) assay was developed to detect and quantify P. neglectus from DNA extracts of soil. The primers, designed from the internal transcribed spacer region of rDNA, showed high specificity with a single melt curve peak to DNA from eight isolates of P. neglectus but did not amplify DNA from 28 isolates of other plant-parasitic and non-plant-parasitic nematodes: A standard curve (R-2 = 0.96; P < 0.001) was generated by amplifying DNA extracted from soil to which nematodes were added. The soil standard curve was validated using sterilized soil inoculated with lower numbers of P. neglectus. A significant positive relationship (R-2 = 0.66; P < 0.001) was observed for nematode numbers quantified from 15 field soils using qPCR and the Whitehead tray and microscopic method but the qPCR generally tended to provide higher estimates. Real-time PCR potentially provides a useful platform for efficient detection and quantification of P. neglectus directly from field soils.Keywords: Verticillium dahliae,
Fragment length polymorphism,
Root lesion nematodes,
Potato cyst nematode,
Northwest United States,
Pacific Northwest,
Polymerase chain reaction,
Genus pratylenchus,
Quantitative detection,
DN
DNA barcoding evidence for the North American presence of alfalfa cyst nematode, Heterodera medicaginis
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