Viewpoint switching in multiview videos using SP-frames

The distinguishing feature of multiview video lies in the interactivity, which allows users to select their favourite viewpoint. It switches bitstream at a particular view when necessary instead of transmitting all the views. The new SP-frame in H.264 is originally developed for multiple bit-rate streaming with the support of seamless switching. The SP-frame can also be directly employed in the viewpoint switching of multiview videos. Notwithstanding the guarantee of seamless switching using SP-frames, the cost is the bulky size of secondary SP-frames. This induces a significant amount of additional space or bandwidth for storage or transmission, especially for the multiview scenario. For this reason, a new motion estimation and compensation technique operating in the quantized transform (QDCT) domain is designed for coding secondary SP-frame in this paper. Our proposed work aims at keeping the secondary SP-frames as small as possible without affecting the size of primary SP-frames by incorporating QDCT-domain motion estimation and compensation in the secondary SP-frame coding. Simulation results show that the size of secondary SP-frames can be reduced remarkably in viewpoint switching. Index Terms — Multiview, viewpoint switching, SP-frame, QDCT-domain, motion estimatio

The Enhanced metastatic potential of hepatocellular carcinoma (HCC) cells with sorafenib resistance

Acquired resistance towards sorafenib treatment was found in HCC patients, which results in poor prognosis. To investigate the enhanced metastatic potential of sorafenib resistance cells, sorafenib-resistant (SorR) cell lines were established by long-term exposure of the HCC cells to the maximum tolerated dose of sorafenib. Cell proliferation assay and qPCR of ABC transporter genes (ABCC1-3) were first performed to confirm the resistance of cells. Migration and invasion assays, and immunoblotting analysis on the expression of epithelial to mesenchymal transition (EMT) regulatory proteins were performed to study the metastatic potential of SorR cells. The expression of CD44 and CD133 were studied by flow cytometry and the gene expressions of pluripotency factors were studied by qPCR to demonstrate the enrichment of cancer stem cells (CSCs) in SorR cells. Control (CTL) and SorR cells were also injected orthotopically to the livers of NOD-SCID mice to investigate the development of lung metastasis. Increased expressions of ABCC1-3 were found in SorR cells. Enhanced migratory and invasive abilities of SorR cells were observed. The changes in expression of EMT regulatory proteins demonstrated an activation of the EMT process in SorR cells. Enriched proportion of CD44+ and CD44+CD133 + cells were also observed in SorR cells. All (8/8) mice injected with SorR cells demonstrated lung metastasis whereas only 1/8 mouse injected with CTL cells showed lung metastasis. HCC cells with sorafenib resistance demonstrated a higher metastatic potential, which may be due to the activated EMT process. Enriched CSCs were also demonstrated in the sorafenib resistant cells. This study suggests that advanced HCC patients with acquired sorafenib resistance may have enhanced tumor growth or distant metastasis, which raises the concern of long-term sorafenib treatment in advanced HCC patients who have developed resistance of sorafenib. © 2013 Chow et al.published_or_final_versio

AluScan: a method for genome-wide scanning of sequence and structure variations in the human genome

<p>Abstract</p> <p>Background</p> <p>To complement next-generation sequencing technologies, there is a pressing need for efficient pre-sequencing capture methods with reduced costs and DNA requirement. The Alu family of short interspersed nucleotide elements is the most abundant type of transposable elements in the human genome and a recognized source of genome instability. With over one million Alu elements distributed throughout the genome, they are well positioned to facilitate genome-wide sequence amplification and capture of regions likely to harbor genetic variation hotspots of biological relevance.</p> <p>Results</p> <p>Here we report on the use of inter-Alu PCR with an enhanced range of amplicons in conjunction with next-generation sequencing to generate an Alu-anchored scan, or 'AluScan', of DNA sequences between Alu transposons, where Alu consensus sequence-based 'H-type' PCR primers that elongate outward from the head of an Alu element are combined with 'T-type' primers elongating from the poly-A containing tail to achieve huge amplicon range. To illustrate the method, glioma DNA was compared with white blood cell control DNA of the same patient by means of AluScan. The over 10 Mb sequences obtained, derived from more than 8,000 genes spread over all the chromosomes, revealed a highly reproducible capture of genomic sequences enriched in genic sequences and cancer candidate gene regions. Requiring only sub-micrograms of sample DNA, the power of AluScan as a discovery tool for genetic variations was demonstrated by the identification of 357 instances of loss of heterozygosity, 341 somatic indels, 274 somatic SNVs, and seven potential somatic SNV hotspots between control and glioma DNA.</p> <p>Conclusions</p> <p>AluScan, implemented with just a small number of H-type and T-type inter-Alu PCR primers, provides an effective capture of a diversity of genome-wide sequences for analysis. The method, by enabling an examination of gene-enriched regions containing exons, introns, and intergenic sequences with modest capture and sequencing costs, computation workload and DNA sample requirement is particularly well suited for accelerating the discovery of somatic mutations, as well as analysis of disease-predisposing germline polymorphisms, by making possible the comparative genome-wide scanning of DNA sequences from large human cohorts.</p

A clinicopathological study of non-functioning pituitary neuroendocrine tumours using the World Health Organization 2022 classification

BackgroundThe 2022 World Health Organization (WHO) classification of pituitary neuroendocrine tumour (PitNET) supersedes the previous one in 2017 and further consolidates the role of transcription factors (TF) in the diagnosis of PitNET. Here, we investigated the clinical utility of the 2022 WHO classification, as compared to that of 2017, in a cohort of patients with non-functioning PitNET (NF-PitNET).MethodsA total of 113 NF-PitNET patients who underwent resection between 2010 and 2021, and had follow-up at Queen Mary Hospital, Hong Kong, were recruited. Surgical specimens were re-stained for the three TF: steroidogenic factor (SF-1), T-box family member TBX19 (TPIT) and POU class 1 homeobox 1 (Pit-1). The associations of different NF-PitNET subtypes with tumour-related outcomes were evaluated by logistic and Cox regression analyses.ResultsBased on the 2022 WHO classification, the majority of NF-PitNET was SF-1-lineage tumours (58.4%), followed by TPIT-lineage tumours (18.6%), tumours with no distinct lineage (16.8%) and Pit-1-lineage tumours (6.2%). Despite fewer entities than the 2017 classification, significant differences in disease-free survival were present amongst these four subtypes (Log-rank test p=0.003), specifically between SF-1-lineage PitNET and PitNET without distinct lineage (Log-rank test p&lt;0.001). In multivariable Cox regression analysis, the subtype of PitNET without distinct lineage (HR 3.02, 95% CI 1.28-7.16, p=0.012), together with tumour volume (HR 1.04, 95% CI 1.01-1.07, p=0.017), were independent predictors of a composite of residual or recurrent disease.ConclusionThe 2022 WHO classification of PitNET is a clinically useful TF and lineage-based system for subtyping NF-PitNET with different tumour behaviour and prognosis

CD9 shapes glucocorticoid sensitivity in pediatric B-cell precursor acute lymphoblastic leukemia

Resistance to glucocorticoids (GCs), the common agents for remission induction in pediatric B-cell precursor acute lymphoblastic leukemia (BCP-ALL), poses a significant therapeutic hurdle. Therefore, dissecting the mechanisms shaping GC resistance could lead to new treatment modalities. Here, we showed that CD9- BCP-ALL cells were preferentially resistant to prednisone and dexamethasone over other standard cytotoxic agents. Concordantly, we identified significantly more poor responders to the prednisone prephase among BCP-ALL patients with a CD9- phenotype, especially for those with adverse presenting features including older age, higher white cell count and BCR-ABL1. Furthermore, gain- and loss-of-function experiments dictated a definitive functional linkage between CD9 expression and GC susceptibility, as demonstrated by the reversal and acquisition of relative GC resistance in CD9low and CD9high BCP-ALL cells, respectively. Despite physical binding to the GC receptor NR3C1, CD9 did not alter its expression, phosphorylation or nuclear translocation but potentiated the induction of GC-responsive genes in GCresistant cells. Importantly, the MEK inhibitor trametinib exhibited higher synergy with GCs against CD9- than CD9+ lymphoblasts to reverse drug resistance in vitro and in vivo. Collectively, our results elucidate a previously unrecognized regulatory function of CD9 in GC sensitivity, and inform new strategies for management of children with resistant BCP-ALL

A base-stabilized silylene-promoted C(sp³)-H borylation and H₂ activation

Treatment of the amidinato amidosilylene [L{(Me3Si)2N}Si:] [1, L = PhC(NtBu)2] with a slight excess of borane-tetrahydrofuran complex [BH3·THF] in toluene at room temperature afforded the silylene-borane adduct [L{(Me3Si)2N}Si:→BH3] (2). A triflate substituent was introduced on the boron center by reacting 2 with methyl triflate [MeOTf] (OTf = OSO2CF3) in toluene at room temperature to form [L{(Me3Si)2N}Si:→BH2OTf] (3), with the elimination of CH4 gas. The intramolecular C(sp3)-H borylation and H2 elimination occurred by reacting complex 3 with 1 in refluxing toluene to form a C-B bond in the resulting silylene-boronium ion 5. Complex 5 activated H2 gas or NH3BH3 at room temperature to form silylene-borane adduct 2 and [L{(Me3Si)2N}Si-H]OTf. Additionally, the reaction of 5 with H2 was studied through density functional theory calculations.Agency for Science, Technology and Research (A*STAR)Ministry of Education (MOE)National Research Foundation (NRF)Accepted versionThis work is supported by an ASTAR SERC PSF grant (1421200081), the Ministry of Education, Academic Research Fund Tier 1 (RG 121/17), and the National Research Foundation Singapore NRF-ANR (NRF2018-NRF-ANR026 Si-POP)

Theoretical examination of competitive β-radical-induced cleavages of N-C

Selective cleavages of N-CThe accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

Charge transfer reactions between gas-phase hydrated electrons, molecular oxygen and carbon dioxide at temperatures of 80-300 K

(VLID)4795251Version of recor

Reductions of Oxygen, Carbon Dioxide, and Acetonitrile by the Magnesium(II)/Magnesium(I) Couple in Aqueous Media: Theoretical Insights from a Nano-Sized Water Droplet

Reductions of O₂, CO₂, and CH₃CN by the half-reaction of the Mg­(II)/Mg­(I) couple (Mg²⁺ + e^– → Mg^+•) confined in a nanosized water droplet ([Mg­(H₂O)₁₆]^•+) have been examined theoretically by means of density functional theory based molecular dynamics methods. The present works have revealed many intriguing aspects of the reaction dynamics of the water clusters within several picoseconds or even in subpicoseconds. The reduction of O₂ requires an overall doublet spin state of the system. The reductions of CO₂ and CH₃CN are facilitated by their bending vibrations and the electron-transfer processes complete within 0.5 ps. For all reactions studied, the radical anions, i.e., O₂^•–, CO₂^•–, and CH₃CN^•–, are initially formed on the cluster surface. O₂^•– and CO₂^•– can integrate into the clusters due to their high hydrophilicity. They are either solvated in the second solvation shell of Mg²⁺ as a solvent-separated ion pair (ssip) or directly coordinated to Mg²⁺ as a contact-ion pair (cip) having the ¹η-[MgO₂]^•+ and ¹η-[MgOCO]^•+ coordination modes. The ¹η-[MgO₂]^•+ core is more crowded than the ¹η-[MgOCO]^•+ core. The reaction enthalpies of the formation of ssip and cip of [Mg­(CO₂)­(H₂O)₁₆]^•+ are −36 ± 4 kJ mol^–1 and −30 ± 9 kJ mol^–1, respectively, which were estimated based on the average temperature changes during the ion–molecule reaction between CO₂ and [Mg­(H₂O)₁₆]^•+. The values for the formation of ssip and cip of [Mg­(O₂)­(H₂O)₁₆]^•+ are estimated to be −112 ± 18 kJ mol^–1 and −128 ± 28 kJ mol^–1, respectively. CH₃CN^•– undergoes protonation spontaneously to form the hydrophobic [CH₃CN, H]^•. Both CH₃CN and [CH₃CN, H]^• cannot efficiently penetrate into the clusters with activation barriers of 22 kJ mol^–1 and ∼40 kJ mol^–1, respectively. These results provide fundamental insights into the solvation dynamics of the Mg²⁺/Mg^•+ couple on the molecular level

Formation of n -&gt; pi(+)interaction facilitating dissociative electron transfer in isolated tyrosine-containing molecular peptide radical cations

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