1,494 research outputs found
Fibroblast Growth Factor-9 Enhances M2 Macrophage Differentiation and Attenuates Adverse Cardiac Remodeling in the Infarcted Diabetic Heart
Inflammation has been implicated as a perpetrator of diabetes and its associated complications. Monocytes, key mediators of inflammation, differentiate into pro-inflammatory M1 macrophages and anti-inflammatory M2 macrophages upon infiltration of damaged tissue. However, the inflammatory cell types, which propagate diabetes progression and consequential adverse disorders, remain unclear. The current study was undertaken to assess monocyte infiltration and the role of fibroblast growth factor-9 (FGF-9) on monocyte to macrophage differentiation and cardioprotection in the diabetic infarcted heart. Db/db diabetic mice were assigned to sham, myocardial infarction (MI), and MI+FGF-9 groups. MI was induced by permanent coronary artery ligation and animals were subjected to 2D transthoracic echocardiography two weeks post-surgery. Immunohistochemical and immunoassay results from heart samples collected suggest significantly increased infiltration of monocytes (Mean +/- SEM; MI: 2.02% +/- 0.23% vs. Sham 0.75% +/- 0.07%; p \u3c 0.05) and associated pro-inflammatory cytokines (TNF-alpha, MCP-1, and IL-6), adverse cardiac remodeling (Mean +/- SEM; MI: 33% +/- 3.04% vs. Sham 2.2% +/- 0.33%; p \u3c 0.05), and left ventricular dysfunction (Mean +/- SEM; MI: 35.4% +/- 1.25% vs. Sham 49.19% +/- 1.07%; p \u3c 0.05) in the MI group. Importantly, treatment of diabetic infarcted myocardium with FGF-9 resulted in significantly decreased monocyte infiltration (Mean +/- SEM; MI+FGF-9: 1.39% +/- 0.1% vs. MI: 2.02% +/- 0.23%; p \u3c 0.05), increased M2 macrophage differentiation (Mean +/- SEM; MI+FGF-9: 4.82% +/- 0.86% vs. MI: 0.85% +/- 0.3%; p \u3c 0.05) and associated anti-inflammatory cytokines (IL-10 and IL-1RA), reduced adverse remodeling (Mean +/- SEM; MI+FGF-9: 11.59% +/- 1.2% vs. MI: 33% +/- 3.04%; p \u3c 0.05), and improved cardiac function (Fractional shortening, Mean +/- SEM; MI+FGF-9: 41.51% +/- 1.68% vs. MI: 35.4% +/- 1.25%; p \u3c 0.05). In conclusion, our data suggest FGF-9 possesses novel therapeutic potential in its ability to mediate monocyte to M2 differentiation and confer cardiac protection in the post-MI diabetic heart
Nonlinear dynamics of the interface of dielectric liquids in a strong electric field: Reduced equations of motion
The evolution of the interface between two ideal dielectric liquids in a
strong vertical electric field is studied. It is found that a particular flow
regime, for which the velocity potential and the electric field potential are
linearly dependent functions, is possible if the ratio of the permittivities of
liquids is inversely proportional to the ratio of their densities. The
corresponding reduced equations for interface motion are derived. In the limit
of small density ratio, these equations coincide with the well-known equations
describing the Laplacian growth.Comment: 10 page
Drug utilization study in diabetic patients seeking medical treatment in a north Indian rural medical college hospital
Background: Diabetes Mellitus is a chronic disease and its life-long management causes burden on lifestyle and financial condition of the patients. Drug utilization studies provide useful insights into the current prescribing practices.Methods: To evaluate the drug utilization pattern of anti-diabetic drugs in diabetic patients. A prospective observational study was carried out in adult diabetic patients visiting the Wards and Outpatient Department of General Medicine of a tertiary care hospital. The demographic data and utilization of different classes of anti-diabetic agents as well as individual drugs were analyzed.Results: In 125 patients (Male-65, Female-60), a total of 379 drugs (average 3.032±2.05) were used per day, out of which 76 (20.05%) were rational fixed dose combinations (FDCs) and 261 (68.86%) were prescribed from National List of Essential Medicines (NLEM) 2015. The number of drugs prescribed to be ingested was 326 (86.01%) and 63 (16.62%) were injectables.Conclusions: It was found that the prescription tendencies of the doctors were quite rational. More improvement can be done by sensitizing them to prescribe more drugs from NLEM. The limitations in the affordability of rural population should be taken care of while prescribing drugs for this chronic disease
Development of Some Larval Nematodes in Experimental and Natural Animal Hosts: An Insight into Development of Pathological Lesions vis-a-vis Host-Parasite Interactions
Infective third-stage larvae of three spiruroid nematodes, Ascarops strongylina and Physocephalus sexalatus of pigs and Spirocerca lupi of dogs, were recovered from 14 species of coprophagous beetles belonging to 4 different genera. These larvae were fed to rabbits and/or guinea pigs to study their development in these experimental hosts. Larvae of A. strongylina reached the adult stage in all rabbits and one guinea pig. The adult worms recovered in these hosts were 40% and 4%, respectively, and became diminutive in comparison to their natural hosts. The larvae of P. sexalatus became reencysted in the gastric wall of rabbits inducing marked pathological changes. The infective larvae of S. lupi became reencapsulated in the stomach wall of the rabbit and also showed development in the aortic wall. Adults of Toxocara canis of dog, collected from 5 different regions of the Indian subcontinent, varied significantly in size. The mouse passage of infective larvae of one of these types led to the recovery of the adults from the experimental dogs that were smaller in size and caused severe pathology in natural experimental hosts. Developmental effects shown in experimental hosts and host specificity are of value in understanding the evolution of nematode parasitism
Regulation of PTEN/Akt Pathway Enhances Cardiomyogenesis and Attenuates Adverse Left Ventricular Remodeling following Thymosin beta 4 Overexpressing Embryonic Stem Cell Transplantation in the Infarcted Heart
Thymosin beta 4 (T beta 4), a small G-actin sequestering peptide, mediates cell proliferation, migration, and angiogenesis. Whether embryonic stem (ES) cells, overexpressing T beta 4, readily differentiate into cardiac myocytes in vitro and in vivo and enhance cardioprotection following transplantation post myocardial infarction (MI) remains unknown. Accordingly, we established stable mouse ES cell lines, RFP-ESCs and T beta 4-ESCs, expressing RFP and an RFP-T beta 4 fusion protein, respectively. In vitro, the number of spontaneously beating embryoid bodies (EBs) was significantly increased in T beta 4-ESCs at day 9, 12 and 15, compared with RFP-ESCs. Enhanced expression of cardiac transcriptional factors GATA-4, Mef2c and Txb6 in T beta 4-EBs, as confirmed with real time-PCR analysis, was accompanied by the increased number of EB areas stained positive for sarcomeric alpha-actin in T beta 4-EBs, compared with the RFP control, suggesting a significant increase in functional cardiac myocytes. Furthermore, we transplanted T beta 4-ESCs into the infarcted mouse heart and performed morphological and functional analysis 2 weeks after MI. There was a significant increase in newly formed cardiac myocytes associated with the Notch pathway, a decrease in apoptotic nuclei mediated by an increase in Akt and a decrease in levels of PTEN. Cardiac fibrosis was significantly reduced, and left ventricular function was significantly augmented in the T beta 4-ESC transplanted group, compared with controls. It is concluded that genetically modified T beta 4-ESCs, potentiates their ability to turn into cardiac myocytes in vitro as well as in vivo. Moreover, we also demonstrate that there was a significant decrease in both cardiac apoptosis and fibrosis, thus improving cardiac function in the infarcted heart
Extreme timescale core-level spectroscopy with tailored XUV pulses
A new approach for few-femtosecond time-resolved photoelectron spectroscopy
in condensed matter that balances the combined needs for both temporal and
energy resolution is demonstrated. Here, the method is designed to investigate
a prototypical Mott insulator, tantalum disulphide (1T-TaS2), which transforms
from its charge-density-wave ordered Mott insulating state to a conducting
state in a matter of femtoseconds. The signature to be observed through the
phase transition is a charge-density-wave induced splitting of the Ta 4f
core-levels, which can be resolved with sub-eV spectral resolution. Combining
this spectral resolution with few-femtosecond time resolution enables the
collapse of the charge ordered Mott state to be clocked. Precise knowledge of
the sub-20-femtosecond dynamics will provide new insight into the physical
mechanism behind the collapse and may reveal Mott physics on the timescale of
electronic hopping.Comment: 20 pages, 6 figure
Structural Color Production in Melanin-based Disordered Colloidal Nanoparticle Assemblies in Spherical Confinement
Melanin is a ubiquitous natural pigment that exhibits broadband absorption
and high refractive index. Despite its widespread use in structural color
production, how the absorbing material, melanin, affects the generated color is
unknown. Using a combined molecular dynamics and finite-difference time-domain
computational approach, this paper investigates structural color generation in
one-component melanin nanoparticle-based supra-assemblies (called supraballs)
as well as binary mixtures of melanin and silica (non-absorbing)
nanoparticle-based supraballs. Experimentally produced one-component melanin
and one-component silica supraballs, with thoroughly characterized primary
particle characteristics using neutron scattering, produce reflectance profiles
similar to the computational analogues, confirming that the computational
approach correctly simulates both absorption and multiple scattering from the
self-assembled nanoparticles. These combined approaches demonstrate that
melanin's broadband absorption increases the primary reflectance peak
wavelength, increases saturation, and decreases lightness factor. In addition,
the dispersity of nanoparticle size more strongly influences the optical
properties of supraballs than packing fraction, as evidenced by production of a
larger range of colors when size dispersity is varied versus packing fraction.
For binary melanin and silica supraballs, the chemistry-based stratification
allows for more diverse color generation and finer saturation tuning than does
the degree of mixing/demixing between the two chemistries.Comment: 40 pages, Figure
Modeling Structural Colors from Disordered One-Component Colloidal Nanoparticle-based Supraballs using Combined Experimental and Simulation Techniques
Bright, saturated structural colors in birds have inspired synthesis of
self-assembled, disordered arrays of assembled nanoparticles with varied
particle spacings and refractive indices. However, predicting colors of
assembled nanoparticles, and thereby guiding their synthesis, remains
challenging due to the effects of multiple scattering and strong absorption.
Here, we use a computational approach to first reconstruct the nanoparticles'
assembled structures from small-angle scattering measurements and then input
the reconstructed structures to a finite-difference time-domain method to
predict their color and reflectance. This computational approach is
successfully validated by comparing its predictions against experimentally
measured reflectance and provides a pathway for reverse engineering colloidal
assemblies with desired optical and photothermal properties.Comment: 14 pages, 3 figures, 1 ToC figur
Mechanism of Structural Colors in Binary Mixtures of Nanoparticle-based Supraballs
Inspired by structural colors in avian species, various synthetic strategies
have been developed to produce non-iridescent, saturated colors using
nanoparticle assemblies. Mixtures of nanoparticles varying in particle
chemistry (or complex refractive indices) and particle size have additional
emergent properties that impact the color produced. For such complex
multi-component systems, an understanding of assembled structure along with a
robust optical modeling tool can empower scientists to perform intensive
structure-color relationship studies and fabricate designer materials with
tailored color. Here, we demonstrate how we can reconstruct the assembled
structure from small-angle scattering measurements using the computational
reverse-engineering analysis for scattering experiments (CREASE) method and
then use the reconstructed structure in finite-difference time-domain (FDTD)
calculations to predict color. We successfully, quantitatively predict
experimentally observed color in mixtures containing strongly absorbing melanin
nanoparticles and demonstrate the influence of a single layer of segregated
nanoparticles on color produced. The versatile computational approach presented
in this work is useful for engineering synthetic materials with desired colors
without laborious trial and error experiments.Comment: 23 Pages, 5 Figures, 1 ToC Figur
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