Results of monitoring at the British library excavation

The main phase of excavation for the basements of the British Library at St Pancras, London, was completed in 1987. The project included basements extending up to 25 m deep, through the London Clay and into the Lambeth Group. The excavations were formed using both the top-down method and open excavation with ground anchors. Existing major buildings lie within 25 m of the site and London Underground tunnels lie below and adjacent to the site. The purpose of this paper is to present the results of displacement monitoring; they are summarised in the paper and presented in more detail in online supplementary data files. The retaining walls advanced towards the site by up to about 32 mm and the clays expanded rapidly on unloading beneath the excavations, causing the Victoria Line tunnels to heave by up to 22 mm. The slow progress of the project provided an unusual opportunity to monitor ground and structure movements in the surroundings before site activity began. Ironically, it was found that the largest settlements of adjacent buildings were caused by the installation of equipment intended to measure the settlements. Extensive condition surveys were carried out, but no damage to adjacent structures or tunnels has been recorded. </jats:p

Constituent Load Estimation in the Lower Ouachita-Smackover Watershed

Water quality was monitored at 21 sites in the Lower Ouachita‐Smackover Watershed from 2013 November through 2014 September. The U.S. Geological Survey maintains discharge monitoring stations at two of these sites, Moro Creek (USGS 07362500) and Smackover Creek (USGS 07362100), which were sampled during base flow and storm event conditions, whereas the other sites were only sampled during baseflow. The Arkansas Water Resources Center (AWRC) estimated constituent loads for nitrate‐N (NO₃‐–N), total nitrogen (TN), soluble reactive phosphorus (SRP), total phosphorus (TP) and total suspended solids (TSS) using the U.S. Geological Survey LOADEST software. LOADEST creates regression models between constituent concentrations and discharge, as well as time. The resulting models were applied to daily discharge throughout calendar years 2013 and 2014 to estimate loads. Annual and monthly loads and flow volumes for each site are summarized in this report

Constituent Loads and Trends in the Upper Illinois River Watershed and Upper White River Basin

Water chemistry can greatly influence the quality of surface waters and affect the ability for streams and rivers to meet their designated use(s). In Arkansas, many streams and rivers were placed on the 2008 303(d) list of impaired water bodies due to excess levels of nutrients, chlorides, sulfates, and sediments (ADEQ, 2008). These constituents continue to be listed as the potential cause for water‐quality impairments through the most recent draft 303(d) list (ADEQ, 2014). The Arkansas Non‐Point Source (NPS) Management Program wants to reduce poll‐ utant loading from the landscape and improve water quality, where funding for projects is targeted to priority watersheds throughout the State

The environments of Markarian galaxies

The extensively studied Markarian sample of 1500 ultraviolet excess galaxies contains many Seyfert, starburst, and peculiar galaxies. Using the 20 minute V plates obtained for the construction of the Hubble Space Telescope Guide Star Catalog, the authors investigated the morphologies of the Markarian galaxies and the environments in which they are located. The relationship between the types of nuclear activity and the morphologies and environments of the Markarian galaxies is discussed. The authors conclude that the type of nuclear activity present in the galaxies of the Markarian sample is not dependent on either the morphology or the local environment of the galaxy. This is not to imply that nuclear activity per se is not influenced by the environment in which the nucleus is located. Rather the type of nuclear activity (at least in the Markarian population) does not appear to be determined by the environment

Beaver Lake Numeric Chlorophyll-a and Secchi Transparency Standards, Phases II and III: Uncertainty and Trend Analysis

The objective of Phases II and III of this study were to 1) assess the variation in chl‐a and ST across multiple spatial and temporal scales in Beaver Lake in order to validate the assessment method, and 2) quantify trends in chl‐a, ST, and nutrient (total phosphorus and total nitrogen) concentrations in Beaver Lake and the major inflowing rivers to verify any potential water quality impairment

Mutation of Arabidopsis SPLICEOSOMAL TIMEKEEPER LOCUS1 Causes Circadian Clock Defects

The circadian clock plays a crucial role in coordinating plant metabolic and physiological functions with predictable environmental variables, such as dusk and dawn, while also modulating responses to biotic and abiotic challenges. Much of the initial characterization of the circadian system has focused on transcriptional initiation, but it is now apparent that considerable regulation is exerted after this key regulatory step. Transcript processing, protein stability, and cofactor availability have all been reported to influence circadian rhythms in a variety of species. We used a genetic screen to identify a mutation within a putative RNA binding protein (SPLICEOSOMAL TIMEKEEPER LOCUS1 [STIPL1]) that induces a long circadian period phenotype under constant conditions. STIPL1 is a homolog of the spliceosomal proteins TFP11 (Homo sapiens) and Ntr1p (Saccharomyces cerevisiae) involved in spliceosome disassembly. Analysis of general and alternative splicing using a high-resolution RT-PCR system revealed that mutation of this protein causes less efficient splicing of most but not all of the introns analyzed. In particular, the altered accumulation of circadian-associated transcripts may contribute to the observed mutant phenotype. Interestingly, mutation of a close homolog of STIPL1, STIP-LIKE2, does not cause a circadian phenotype, which suggests divergence in function between these family members. Our work highlights the importance of posttranscriptional control within the clock mechanism. © 2012 American Society of Plant Biologists. All rights reserved

The Application of CRISPR Technology to High Content Screening in Primary Neurons

Axon growth is coordinated by multiple interacting proteins that remain incompletely characterized. High content screening (HCS), in which manipulation of candidate genes is combined with rapid image analysis of phenotypic effects, has emerged as a powerful technique to identify key regulators of axon outgrowth. Here we explore the utility of a genome editingapproach referred to as CRISPR (Clustered Regularly Interspersed Palindromic Repeats) for knockout screening in primary neurons. In the CRISPR approach a DNA-cleaving Cas enzyme is guided to genomic target sequences by user-created guide RNA (sgRNA), where it initiates a double-stranded break that ultimately results in frameshift mutation and loss of protein production. Using electroporation of plasmid DNA that co-expresses Cas9enzyme and sgRNA, we first verified the ability of CRISPR targeting to achieve protein-level knockdown in cultured postnatal cortical neurons. Targeted proteins included NeuN (RbFox3) and PTEN, a well-studied regulator of axon growth. Effective knockdown lagged at least four days behind transfection, but targeted proteins were eventually undetectable by immunohistochemistry in \u3e 80% of transfected cells. Consistent with this, anti-PTEN sgRNA produced no changes in neurite outgrowth when assessed three days post-transfection. When week-long cultures were replated, however, PTEN knockdown consistently increased neurite lengths. These CRISPR-mediated PTEN effects were achieved using multi-well transfection and automated phenotypic analysis, indicating the suitability of PTEN as a positive control for future CRISPR-based screening efforts. Combined, these data establish an example of CRISPR-mediated protein knockdown in primary cortical neurons and its compatibility with HCS workflows

Composition of dissolved organic matter within a lacustrine environment

Freshwater dissolved organic matter (DOM) is a complex mixture of chemical components that are central to many environmental processes, including carbon and nitrogen cycling. However, questions remain as to its chemical characteristics, sources and transformation mechanisms. Here, we employ 1- and 2-D nuclear magnetic resonance (NMR) spectroscopy to investigate the structural components of lacustrine DOM from Ireland, and how it varies within a lake system, as well as to assess potential sources. Major components found, such as carboxyl-rich alicyclic molecules (CRAM) are consistent with those recently identified in marine and freshwater DOM. Lignin-type markers and protein/peptides were identified and vary spatially. Phenylalanine was detected in lake areas influenced by agriculture, whereas it is not detectable where zebra mussels are prominent. The presence of peptidoglycan, lipoproteins, large polymeric carbo- hydrates and proteinaceous material supports the substantial contribution of material derived from microorganisms. Evidence is provided that peptidoglycan and silicate species may in part originate from soil microbes

Five year prognosis in patients with angina identified in primary care : incident cohort study

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