The Histone 3'-Terminal Stem-Loop-Binding Protein Enhances Translation through a Functional and Physical Interaction with Eukaryotic Initiation Factor 4G (eIF4G) and eIF3

Metazoan cell cycle-regulated histone mRNAs are unique cellular mRNAs in that they terminate in a highly conserved stem-loop structure instead of a poly(A) tail. Not only is the stem-loop structure necessary for 3'-end formation but it regulates the stability and translational efficiency of histone mRNAs. The histone stem-loop structure is recognized by the stem-loop-binding protein (SLBP), which is required for the regulation of mRNA processing and turnover. In this study, we show that SLBP is required for the translation of mRNAs containing the histone stem-loop structure. Moreover, we show that the translation of mRNAs ending in the histone stem-loop is stimulated in Saccharomyces cerevisiae cells expressing mammalian SLBP. The translational function of SLBP genetically required eukaryotic initiation factor 4E (eIF4E), eIF4G, and eIF3, and expressed SLBP coisolated with S. cerevisiae initiation factor complexes that bound the 5' cap in a manner dependent on eIF4G and eIF3. Furthermore, eIF4G coimmunoprecipitated with endogenous SLBP in mammalian cell extracts and recombinant SLBP and eIF4G coisolated. These data indicate that SLBP stimulates the translation of histone mRNAs through a functional interaction with both the mRNA stem-loop and the 5' cap that is mediated by eIF4G and eIF3

Mage - Reactive articulatory feature control of HMM-based parametric speech synthesis

In this paper, we present the integration of articulatory control into MAGE, a framework for realtime and interactive (reactive) parametric speech synthesis using hidden Markov models (HMMs). MAGE is based on the speech synthesis engine from HTS and uses acoustic features (spectrum and f0) to model and synthesize speech. In this work, we replace the standard acoustic models with models combining acoustic and articulatory features, such as tongue, lips and jaw positions. We then use feature-space-switched articulatory-to-acoustic regression matrices to enable us to control the spectral acoustic features by manipulating the articulatory features. Combining this synthesis model with MAGE allows us to interactively and intuitively modify phones synthesized in real time, for example transforming one phone into another, by controlling the configuration of the articulators in a visual display. Index Terms: speech synthesis, reactive, articulators 1

Robustness of HMM-based Speech Synthesis

As speech synthesis techniques become more advanced, we are able to consider building high-quality voices from data collected outside the usual highly-controlled recording studio environment. This presents new challenges that are not present in conventional text-to-speech synthesis: the available speech data are not perfectly clean, the recording conditions are not consistent, and/or the phonetic balance of the material is not ideal. Although a clear picture of the performance of various speech synthesis techniques (e.g., concatenative, HMM-based or hybrid) under good conditions is provided by the Blizzard Challenge, it is not well understood how robust these algorithms are to less favourable conditions. In this paper, we analyse the performance of several speech synthesis methods under such conditions. This is, as far as we know, a new research topic: ``Robust speech synthesis.'' As a consequence of our investigations, we propose a new robust training method for the HMM-based speech synthesis in for use with speech data collected in unfavourable conditions

Structural Holes and Online Bridging: A qualitative study of the Social Structures surrounding Academic Entrepreneurs and Technology Entrepreneurs in the East Midlands region of the United Kingdom

This management project is divided into two parts that explore the social structures surrounding academic entrepreneurs and technology entrepreneurs in the East Midlands region of the United Kingdom. The first part researches the nature of the social structures surrounding the two entrepreneurial groups and the second part produces a business plan to exploit opportunities present within these social structures. Part A: Structural Holes Research The aim of the research was to use social capital theory as a framework to analyse the social structures surrounding academic entrepreneurs and technology entrepreneurs during early stage commercial activity, in order to provide an overall view of the nature of the social structures and identify the issues present within each inhibiting economic development. To achieve this information from the available published literature was compared to the findings from a series of interviews held with entrepreneurs and other members of the social structure. The outcome of the research was the production of two models of the social structures surrounding each type of entrepreneur as well as the identification of a number of issues that effect early stage commercial activity within the East Midlands region. The three most significant issues resulted as a pre-seed funding gap that inhibits the level of technology entrepreneurship, a research commercialisation gap that reduces the level of academic entrepreneurial activity, and an early stage management support availability gap that hinders access to early stage expertise, knowledge, and advice. Part B: Online Bridging Business Plan The aim of the business plan was to use the research in part A to develop a proposal for a specialist online network that utilises a combination of tools to overcome issues within the social structures to increase member interaction across the network. The business plan provided is a complete and rigorous document that includes a description of the business and the proposed service offering, an analysis of the market environment, detail of the business strategy and objectives, a proposition for investment, identification of key risks, and a complete set of financials for five years

A review of ecogeochemistry approaches to estimating movements of marine animals

Author Posting. © Association for the Sciences of Limnology and Oceanography, 2013. This article is posted here by permission of Association for the Sciences of Limnology and Oceanography for personal use, not for redistribution. The definitive version was published in Limnology and Oceanography 58 (2013): 697-714, doi:10.4319/lo.2013.58.2.0697.Ecogeochemistry—the application of geochemical techniques to fundamental questions in population and community ecology—has been used in animal migration studies in terrestrial environments for several decades; however, the approach has received far less attention in marine systems. This review includes comprehensive meta-analyses of organic zooplankton δ13C and δ15N values at the base of the food web, dissolved inorganic carbon δ13C values, and seawater δ18O values to create, for the first time, robust isoscapes for the Atlantic Ocean. These isoscapes present far greater geographic variability in multiple geochemical tracers than was previously thought, thus forming the foundation for reconstructions of habitat use and migration patterns of marine organisms. We review several additional tracers, including trace-element-to-calcium ratios and heavy element stable isotopes, to examine anadromous migrations. We highlight the value of the ecogeochemistry approach by examining case studies on three components of connectivity: dispersal and natal homing, functional connectivity, and migratory connectivity. We also discuss recent advances in compound-specific stable carbon and nitrogen isotope analyses for tracking animal movement. A better understanding of isotopic routing and fractionation factors, particularly of individual compound classes, is necessary to realize the full potential of ecogeochemistry.We were supported by funding from the National Science Foundation (Division of Ocean Sciences–0825148 to S.R.T.), Award USA 00002 and KSA 00011 from the King Abdullah University of Science and Technology (to S.R.T.), and a National Science Foundation Graduate Research Fellowship (to L.H.)

Topological Photonics

Topological photonics is a rapidly emerging field of research in which geometrical and topological ideas are exploited to design and control the behavior of light. Drawing inspiration from the discovery of the quantum Hall effects and topological insulators in condensed matter, recent advances have shown how to engineer analogous effects also for photons, leading to remarkable phenomena such as the robust unidirectional propagation of light, which hold great promise for applications. Thanks to the flexibility and diversity of photonics systems, this field is also opening up new opportunities to realize exotic topological models and to probe and exploit topological effects in new ways. This article reviews experimental and theoretical developments in topological photonics across a wide range of experimental platforms, including photonic crystals, waveguides, metamaterials, cavities, optomechanics, silicon photonics, and circuit QED. A discussion of how changing the dimensionality and symmetries of photonics systems has allowed for the realization of different topological phases is offered, and progress in understanding the interplay of topology with non-Hermitian effects, such as dissipation, is reviewed. As an exciting perspective, topological photonics can be combined with optical nonlinearities, leading toward new collective phenomena and novel strongly correlated states of light, such as an analog of the fractional quantum Hall effect.Comment: 87 pages, 30 figures, published versio

Low-temperature thermochronology of francolite: Insights into timing of Dead Sea Transform motion

Cambrian siliciclastic sequences along the Dead Sea Transform (DST) margin in southern Israel and southern Jordan host both detrital fluorapatite [D‐apatite] and U‐rich authigenic carbonate‐fluorapatite (francolite) [A‐apatite]. D‐apatite and underlying Neoproterozoic basement apatite yield fission track (FT) data reflecting Paleozoic‐Mesozoic sedimentary cycles and epeirogenic events, and dispersed (U‐Th‐Sm)/He (AHe) ages. A‐apatite, which may partially or completely replace D‐apatite, yields an early Miocene FT age suggesting formation by fracturing, hydrothermal fluid ascent and intra‐strata recrystallisation, linked to early DST motion. The DST, separating the African and Arabian plates, records ~105 km of sinistral strike‐slip displacement, but became more transtensional post‐5 Ma. Helium diffusion measurements on A‐apatite are consistent with thermally activated volume diffusion, indicating Tc ~52‐56±10°C (cooling rate 10°C/Myr). A‐apatite AHe data record Pliocene cooling (~35‐40°C) during the transtensional phase of movement. This suggests that timing of important milestones in DST motion can be discerned using A‐apatite low‐temperature thermochronology data alone

Low-temperature thermochronology of francolite: Insights into timing of Dead Sea Transform motion

Cambrian siliciclastic sequences along the Dead Sea Transform (DST) margin in southern Israel and southern Jordan host both detrital fluorapatite [D‐apatite] and U‐rich authigenic carbonate‐fluorapatite (francolite) [A‐apatite]. D‐apatite and underlying Neoproterozoic basement apatite yield fission track (FT) data reflecting Paleozoic‐Mesozoic sedimentary cycles and epeirogenic events, and dispersed (U‐Th‐Sm)/He (AHe) ages. A‐apatite, which may partially or completely replace D‐apatite, yields an early Miocene FT age suggesting formation by fracturing, hydrothermal fluid ascent and intra‐strata recrystallisation, linked to early DST motion. The DST, separating the African and Arabian plates, records ~105 km of sinistral strike‐slip displacement, but became more transtensional post‐5 Ma. Helium diffusion measurements on A‐apatite are consistent with thermally activated volume diffusion, indicating Tc ~52‐56±10°C (cooling rate 10°C/Myr). A‐apatite AHe data record Pliocene cooling (~35‐40°C) during the transtensional phase of movement. This suggests that timing of important milestones in DST motion can be discerned using A‐apatite low‐temperature thermochronology data alone

Quantifying the resolution of spatial and temporal representation in children with 22q11.2 deletion syndrome.

ObjectivesOur ability to generate mental representation of magnitude from sensory information affects how we perceive and experience the world. Reduced resolution of the mental representations formed from sensory inputs may generate impairment in the proximal and distal information processes that utilize these representations. Impairment of spatial and temporal information processing likely underpins the non-verbal cognitive impairments observed in 22q11.2 deletion syndrome (22q11DS). The present study builds on prior research by seeking to quantify the resolution of spatial and temporal representation in children with 22q11DS, sex chromosome aneuploidy (SCA), and a typically developing (TD) control group.Participants and methodsChildren (22q11DS = 70, SCA = 49, TD = 46) responded to visual or auditory stimuli with varying difference ratios. The participant's task was to identify which of two sequentially presented stimuli was of larger magnitude in terms of, size, duration, or auditory frequency. Detection threshold was calculated as the minimum difference ratio between the "standard" and the "target" stimuli required to achieve 75% accuracy in detecting that the two stimuli were different.ResultsChildren with 22q11DS required larger magnitude difference between spatial stimuli for accurate identification compared with both the SCA and TD groups (% difference from standard: 22q11DS = 14; SCA = 8; TD: 7; F  = 8.42, p < 0.001). Temporal detection threshold was also higher for the 22q11DS group to both visual (% difference from standard: 22q11DS = 14; SCA = 8; TD = 7; F  = 8.33, p < 0.001) and auditory (% difference from standard: 22q11DS = 23; SCA = 12; TD: 8; F  = 8.99, p < 0.001) stimuli compared with both the SCA and TD groups, while the SCA and TD groups displayed equivalent performance on these measures (p's > 0.05). Pitch detection threshold did not differ among the groups (p's > 0.05).ConclusionsThe observation of higher detection thresholds to spatial and temporal stimuli indicates further evidence for reduced resolution in both spatial and temporal magnitude representation in 22q11DS, that does not extend to frequency magnitude representation (pitch detection), and which is not explained by generalized cognitive impairment alone. These findings generate further support for the hypothesis that spatiotemporal hypergranularity of mental representations contributes to the non-verbal cognitive impairment seen in 22q11DS