4 research outputs found
A) PCB chemical structure with labeled (A-D) pyrolle rings (oxygen atoms are in red and nitrogen in blue); B) Schematic of HSA structure with domains and ligand binding sites labeled; Radius of gyration (Rg) values (C) and 2D RMDS plots for free HSA (D), HSA-PCB(IIA) (E), and HSA-PCB(IB) (F) during 300 ns molecular dynamic simulation.
<p>A) PCB chemical structure with labeled (A-D) pyrolle rings (oxygen atoms are in red and nitrogen in blue); B) Schematic of HSA structure with domains and ligand binding sites labeled; Radius of gyration (Rg) values (C) and 2D RMDS plots for free HSA (D), HSA-PCB(IIA) (E), and HSA-PCB(IB) (F) during 300 ns molecular dynamic simulation.</p
Calculated RMSF values (Ă…) from molecular dynamics simulation of free HSA, HSA-PCB(IB), and HSA-PCB(IIA).
<p>RMSF values along whole protein sequence (A), sum of RMSF values of each HSA domain (B), and difference of RMSF values between PCB-HSA complexes and free HSA, with marked amino acid (aa) residues involved in PCB binding for site IB (C), and for site IIA (D).</p
(A) Temperature dependence of 0.5 μM HSA ellipticity at 222 nm in the presence and absence of 0.5 μM PCB; (B) Temperature dependence of 1 μM HSA fluorescence at 340 nm in the presence and absence of 1 μM PCB (λ<sub>EX</sub> = 280 nm); (C) SDS-PAGE profile after trypsin digestion of 3.8 μM HSA in the presence and absence of 3.8 μM PCB.
<p>Lane K: HSA without trypsin; lane M: MW markers; lanes 1–6 correspond to digestion times 0.5, 2, 5, 10, 30, and 60 min without PCB, respectively; lanes 7–12 correspond to digestion times 0.5, 2, 5, 10, 30 and 60 min with PCB, respectively.</p
Catalogue Maison Bigot [titres “Viens dans ma nacelle” => “Bazeilles”]
Catalogue Maison Bigot, 157 rue du Temple [Paris] : “Romances et chansons nouvelles” (verso “Sommes nous prêts ?”) ; titres : “Viens dans ma nacelle => “Bazeilles” ; quatre colonnes par deux foix (titres, auteurs, compositeurs, genres) ; interprètes crédités dans la colonne “genre” : Marius Richard, Mercadier, Amiati, Debailleul, Paulus, B. Delahaye, Gibert, Garnier [Léon Garnier], d’Aubreuil [Daubreuil ?], Antony [Antony Lassaigne], Duhem [Émile Duhem], Canon [Fernande Canon], Bourgès, Bonnet, E. Faure [Elise Faure], Dlle (Mlle?) Derly, Libert, Caudieux, Leduc, Judith, G. Lange [Gabrielle Lange], Mme Barnoll, Kaiser [Augustine Kaïser], Limat [Louis Limat], Farville, Réval [Jules Réval], Velly [Louis Velly], Plessis [Henri Plessis] ; datation par analyse des titres 1886 (hypothèse à confirmer)