Analysis of mdr1-1Δ mutation of MDR1 gene in the “Cimarron Uruguayo” dog

ABSTRACT Objective. The aim of this paper is to analyze the frequency of the mdr1-1D mutation of the MDR1 gene in a dog sample of the Uruguayan Cimarron breed with the objective of increasing the knowledge of this breed’s genome. Materials and methods. Thirty-six animals of this breed were analyzed. The MDR1 gene region, which includes the location where the mutation would be present, was amplified by PCR. Results. The mutation was not detected in any of the analyzed Uruguayan Cimarron. Conclusions. The lack of described ivermectin intoxication cases in veterinary clinic in this breed is explained by the lack of the mutation object of this study. The sequence studied in Cimarron dogs is kept compared to other breeds, except Collies and related breeds (Border Collie, Bearded Collie, Old English sheepdog)

The search for the mdr1-1Δ mutation of the MDR1 gene in four canine breeds in Uruguay (preliminary study)

Objective. The objective of this study is to analyze the frequency of mdr1-1Δ mutation in German Shepherd, Doberman, Border Collie and Greyhound dog breeds in Uruguay. Materials and methods. A total of 95 animals from the four breeds mentioned above were studied. DNA was isolated from blood using potassium acetate with a subsequent degradation from RNA with RNAsaH. The concentration and quality of the DNA obtained was evaluated with a Nanodrop, ND-1000 spectrophotometer. To determine the presence or absence of the mdr1-1Δ mutation, DNA samples were sent to Gene Seek, Neogen Corporation of Chicago, United States, for genotyping. Results. In all 95 animals studied, the mdr1-1Δ mutation was not present. Conclusions. Based on the preliminary results obtained, other elements that may cause adverse drug reactions must be considered: unidentified mutations in other regions of the MDR1 gene; mutations in other genes involved in the transport of drugs from the same subfamily or another; mutations in enzymes involved in drug metabolism (e.g. Cytochrome P450). Moreover, especially with Border Collies and Greyhounds, it is advisable to increase the number of animals in the study. Key words: Dogs, ivermectine, P-glycoprotein (Source: CAB)

Análisis de dos poblaciones de caninos (Canis familiaris) de la raza cimarrón uruguayo utilizando rapds como marcadores moleculares

Objetivo. Analizar la variabilidad genética en dos poblaciones de caninos de la raza autóctona “Cimarrón uruguayo” provenientes de distintas zonas del País. Materiales y métodos. Se aisló ADN de 40 animales de la raza Cimarrón. Mediante la técnica de PCR se analizaron 11 marcadores moleculares RAPD (amplificación al azar de ADN polimórfico). Resultados. Se detectó un 14.9% de loci polimórficos. Se obtuvo un índice de bandas compartidas de 0.91, una distancia genética de 0.16 e índice promedio de diversidad de Nei’s de 0.08. Conclusiones. Ambas poblaciones presentan una alta homogeneidad e identidad genética para estos marcadores y pertenecerían a un núcleo genético común

Preliminary studies of the genetic structure of “Cimarron uruguayo” dog using microsatellite markers

Objetive. To analyze the population structure, using microsatellite markers in a sample of “Cimarron Uruguayo” dogs. Materials and methods. Thirty dogs were analyzed in different areas of Uruguay with a set of nine molecular microsatellite markers using PCR. The population structure was analyzed using the free distribution software “Structure’’. Results. According to our data, the preliminary results show that it is not possible to establish a subdivision among the animals in the sample. Conclusions. The study supports the hypothesis that the currently existing canines derive from a founding nucleus that took refuge in the Northeastern region of the country. The distribution of the breed among the different areas of Uruguay continues nowadays, so there is no isolation among the different groups of animals, and the exchange is constan