171 research outputs found
Small electron transfer proteins as mediators in enzymatic electrochemical biosensors
The final publication is available at Springer."Electrochemical mediators transfer redox equivalents between the active sites of
enzymes and electrodes and, in this way, trigger bioelectrocatalytic redox
processes. This has been very useful in the development of the so-called second
generation biosensors, where they are able to transduce the catalytic event into an
electrical signal. Among other pre-requisites, redox mediators must be readily
oxidized/reduced at the electrode surface and easily interact with the
biorecognition component. Small chemical compounds (e.g. ferrocene derivatives,
ruthenium or osmium complexes and viologens) are frequently used for this
purpose, but lately, small redox proteins (e.g. horse heart cytochrome c) have also
played the role of redox partners in biosensing applications. In general, the
docking between two complementary proteins introduces a second level of
selectivity to the biosensor and enlarges the list of compounds targeted for
analysis. Moreover, electrochemical interferences are frequently minimized owing
to the small overpotentials achieved. This paper aims to provide an overview of
enzyme biosensors that are mediated by electron transfer proteins. The article
begins with a few considerations on mediated electrochemistry in biosensing
2
systems and proceeds with a detailed description of relevant works concerning the
cooperative use of redox enzymes and biological electron donors/acceptors.
A comprehensive view of nitrite reductases
The authors acknowledge the support of the research centers Applied Molecular Biosciences Unit-UCIBIO, which is co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). CMS acknowledges support from Project LISBOA-01-0145-FEDER-007660 (Microbiologia Molecular, Estrutural e Celular) funded by FEDER funds through COMPETE2020 – Programa Operacional Competitividade e Internacionalização (POCI).
Publisher Copyright:
© 2022 Elsevier B.V.The last years have witnessed a steady increase of social and political awareness for the need of studying, monitoring, and controlling several anthropological activities that are dramatically impacting the environment and human health. The increasing turnover rates of the nitrogen cycle across the Planet are of major concern, so the understanding of the biological, chemical, and physical processes associated with the biogeochemical nitrogen cycle has been attracting the attention of several scientific disciplines. For many years, the primary focus has been the so-called “dissimilatory reduction of nitrate”, which refers to the stepwise conversion of nitrate into molecular nitrogen, closely followed by the assimilatory nitrate reduction pathway, which allow nitrogen incorporation into biomolecules. The contribution of bioinorganic chemists to better understand the enzymology underlying these two branches of the N-cycle has been remarkable. The constant development of mechanistic, structural, and biological tools has been keeping this bioinorganic chemistry field very active, making it a highly relevant research area still today. In this paper, we review the state-of-the-art in both dissimilatory and assimilatory nitrite reducing enzymes, highlighting the structural peculiarities of the different metalloenzymes involved in this step.publishersversionpublishe
Electrocatalysis by heme enzymes—applications in biosensing
Funding Information: The APC was funded by TIMB3 project, European Union's Horizon 2020 Research and Innovation Program grant agreement No 810856. Funding Information: Acknowledgments: We acknowledge the support from Project LISBOA-01-0145-FEDER-007660 (Microbiologia Molecular, Estrutural e Celular) funded by FEDER funds through COMPETE 2020-Programa Operacional Competitividade e Internacionalização (POCI); from FCT—Fundação para a Ciência e a Tecnologia (PTDC/BIA-BFS/31026/2017 and 2020.05017.BD) and from the European Union's Horizon 2020 Research and Innovation Program, through TIMB3 and B-LigZymes projects (grant agreements No 810856 and 824017, respectively). We thank Edilson Galdino for critical reading of the manuscript and helpful discussions. Funding Information: Funding: The APC was funded by TIMB3 project, European Union's Horizon 2020 Research and Innovation Program grant agreement No 810856. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.Heme proteins take part in a number of fundamental biological processes, including oxygen transport and storage, electron transfer, catalysis and signal transduction. The redox chemistry of the heme iron and the biochemical diversity of heme proteins have led to the development of a plethora of biotechnological applications. This work focuses on biosensing devices based on heme proteins, in which they are electronically coupled to an electrode and their activity is determined through the measurement of catalytic currents in the presence of substrate, i.e., the target analyte of the biosensor. After an overview of the main concepts of amperometric biosensors, we address transduction schemes, protein immobilization strategies, and the performance of devices that explore reactions of heme biocatalysts, including peroxidase, cytochrome P450, catalase, nitrite reductase, cytochrome c oxidase, cytochrome c and derived microperoxidases, hemoglobin, and myoglobin. We further discuss how structural information about immobilized heme proteins can lead to rational design of biosensing devices, ensuring insights into their efficiency and long-term stability.publishersversionpublishe
Development of new analytical tools for monitoring of cardiovascular disease markers – towards the detection of homocysteine-thiolactone
Poster presented at the 4th International Conference on Bio-Sensing Technology, 10-13 May 2015, Lisbon, Portuga
New PON1-based biosensor for the detection of homocysteine-thiolactone in human plasma
Poster presented at the XXIII International Symposium on Bioelectrochemistry and Bioenergetics, 14-18 June 2015, Malmo, Sweden
Respiratory versatility in Desulfovibrio desulfuricans ATCC 27774 – a proteomic approach
Poster presented at the Bacterial Electron Transfer Processes and their Regulation Meeting, European Federation of Biotechnology Microbial Physiology Section, 15-18 March 2015, Vimeiro, Portugal
Measuring the cytochrome c nitrite reductase activity—practical considerations on the enzyme assays
Hindawi Publishing Corporation Bioinorganic Chemistry and Applications Volume 2010, Article ID 634597, 8 pagesThe cytochrome c nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC 27774 is able to reduce nitrite to ammonia in
a six-electron transfer reaction. Although extensively characterized from the spectroscopic and structural points-of-view, some of its kinetic aspects are still under explored. In this work the kinetic behaviour of ccNiR has been evaluated in a systematic manner using two different spectrophotometric assays carried out in the presence of different redox mediators and a direct electrochemical approach. Solution assays have proved that the specific activity of ccNiR decreases with the reduction potential of the electronic
carriers and ammonium is always the main product of nitrite reduction. The catalytic parameters were discussed on the basis
of the mediator reducing power and also taking into account the location of their putative docking sites with ccNiR. Due to the
fast kinetics of ccNiR, electron delivering from reduced electron donors is rate-limiting in all spectrophotometric assays, so the estimated kinetic constants are apparent only. Nevertheless, this limitation could be overcome by using a direct electrochemical approach which shows that the binding affinity for nitrite decreases whilst turnover increases with the reductive driving force
Measuring the Cytochrome c
The cytochrome c nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC 27774 is able to reduce nitrite to ammonia in a six-electron transfer reaction. Although extensively characterized from the spectroscopic and structural points-of-view, some of its kinetic aspects are still under explored. In this work the kinetic behaviour of ccNiR has been evaluated in a systematic manner using two different spectrophotometric assays carried out in the presence of different redox mediators and a direct electrochemical approach. Solution assays have proved that the specific activity of ccNiR decreases with the reduction potential of the electronic carriers and ammonium is always the main product of nitrite reduction. The catalytic parameters were discussed on the basis of the mediator reducing power and also taking into account the location of their putative docking sites with ccNiR. Due to the fast kinetics of ccNiR, electron delivering from reduced electron donors is rate-limiting in all spectrophotometric assays, so the estimated kinetic constants are apparent only. Nevertheless, this limitation could be overcome by using a direct electrochemical approach which shows that the binding affinity for nitrite decreases whilst turnover increases with the reductive driving force
Construction of effective disposable biosensors for point of care testing of nitrite
© 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/"In this paper we aim to demonstrate, as a proof-of-concept, the feasibility of the mass production of effective point of care tests for nitrite quantification in environmental, food and clinical samples. Following our previous work on the development of third generation electrochemical biosensors based on the ammonia forming nitrite reductase (ccNiR), herein we reduced the size of the electrodes’ system to a miniaturized format, solved the problem of oxygen interference and performed simple quantification assays in real samples. In particular,
carbon paste screen printed electrodes (SPE) were coated with a ccNiR/carbon ink composite homogenized in organic solvents and cured at low temperatures. The biocompatibility of these chemical and thermal treatments was evaluated by cyclic voltammetry showing that the catalytic performance was higher with the combination acetone and a 40 °C curing temperature. The successful incorporation of the protein in the carbon ink/solvent composite, while remaining catalytically competent, attests for ccNiR’s robustness and suitability for application
in screen printed based biosensors. Because the direct electrochemical reduction of molecular oxygen occurs when electroanalytical measurements are performed at the negative potentials required to activate ccNiR (ca. -0.4 V vs Ag/AgCl), an oxygen scavenging system based on the coupling of glucose oxidase and catalase
activities was successfully used. This enabled the quantification of nitrite in different samples (milk, water, plasma and urine) in a straightforward way and with small error (1 – 6%). The sensitivity of the biosensor towards nitrite reduction under optimized conditions was 0.55 A M-1 cm-2 with a linear response range 0.7 – 370 μM.
Star-Shaped Gold Nanoparticles as Friendly Interfaces for Protein Electrochemistry: the Case Study of Cytochrome c
UID/QUI/50006/2019
POCI‐01‐0145‐FEDER‐007265
UID/Multi/04378/2019
POCI‐01‐0145‐FEDER‐007728
PTDC/NAN‐MAT/30589/2017
grant NORTE‐01‐0145‐FEDER‐000011
SFRH/BD/132057/2017Gold nanostars with an average tip-to-tip length of 52±6 nm were functionalized with different capping agents and used as electrode modification materials for protein electrochemistry. Direct electron transfer between cytochrome c and nanostar-coated pyrolytic graphite electrodes was observed with the protein in solution. The electrochemical response was improved at nanostars functionalized with a 1 : 1 mixture of 11-mercaptoundecanoic acid and 4-mercaptobenzoic acid in comparison with gold nanospheres coated with a similar functionalization. Further immobilization of cytochrome c on pyrolytic graphite while conjugated with the same nanostars guaranteed the maintenance of the protein's native properties, whereas direct adsorption on the bare or nanostar-modified electrodes resulted in an altered conformational state. The pseudo-peroxidase activity of the altered cytochrome c was enhanced in the presence of the nanostars.publishe
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