Atti del MoodleMoot Italia 2021

Atti del MoodleMoot Italia 202113-15 dicembre 201

Atti del MoodleMoot Italia 2017

Atti del MoodleMoot Italia 2017 - Dipartimento di Chimica e Tecnologie del Farmaco - Facolt\ue0 di Farmacia e Medicina - Sapienza Universit\ue0 di Roma - 28-30 settembre 2017.Intranet di ateneo ed e-learning \u2013 mondi integratiSi descrive un\u2019esperienza di integrazione del generico sistema LMS \u201cMoodle\u201d all\u2019interno del panorama gi\ue0 consolidato e complesso dei sistemi informativi dell\u2019Ateneo di Verona con l\u2019obiettivo di \u201ccondizionare\u201d lo strumento standard Moodle ad utilizzare logiche universitarie. L\u2019obiettivo viene raggiunto lasciando sostanzialmente inalterata la piattaforma Moodle ed utilizzando le possibilit\ue0 che lo strumento nativamente gi\ue0 offre nel campo dell\u2019integrazione. Il risultato ottenuto va nell\u2019ottica dell\u2019armonizzazione dei sistemi e del miglioramento dell\u2019esperienza utente. Dal punto di vista pratico il risultato del progetto \ue8 una \u201cIntranet\u201d a disposizione di studenti, docenti e personale, che costituisce un punto di accesso unico e semplificato a tutti i servizi on-line dedicati. La realizzazione del sistema si basa sull\u2019implementazione di uno strato di integrazione basato su Liferay che media ed orchestra l\u2019interazione tra sistemi

Transfer-free electrical insulation of epitaxial graphene from its metal substrate

High-quality, large-area epitaxial graphene can be grown on metal surfaces but its transport properties cannot be exploited because the electrical conduction is dominated by the substrate. Here we insulate epitaxial graphene on Ru(0001) by a step-wise intercalation of silicon and oxygen, and the eventual formation of a SiO$_2$ layer between the graphene and the metal. We follow the reaction steps by x-ray photoemission spectroscopy and demonstrate the electrical insulation using a nano-scale multipoint probe technique.Comment: Accepted for publication in Nano Letter

Migliorare l’accessibilità dei materiali didattici digitali nel contesto universitario: un caso di studio

Negli ultimi anni, le istituzioni e la società contemporanea hanno posto un’attenzione sempre crescente ai temi dell’accessibilità e dell’inclusione, anche seguendo la strada indicata da iniziative internazionali come l'Agenda ONU 2030. Questi temi sono fondamentali nel contesto della formazione universitaria, che mira a creare un ambiente didattico-educativo aperto a tutti. Il presente lavoro illustra un caso di studio che ha coinvolto il Dipartimento di Lingue e Letterature Straniere dell'Università degli Studi di Verona in una sperimentazione triennale sull'uso di tecnologie digitali per creare materiali didattici accessibili. Il contributo esamina i risultati ottenuti, inclusi dati di utilizzo e livello di accessibilità dei documenti, e discute le sfide affrontate, fornendo importanti lezioni apprese e prospettive future

Selective activation of human dendritic cells by OM-85 through a NF-kB and MAPK dependent pathway.

OM-85 (Broncho-Vaxom®, Broncho-Munal®, Ommunal®, Paxoral®, Vaxoral®), a product made of the water soluble fractions of 21 inactivated bacterial strain patterns responsible for respiratory tract infections, is used for the prevention of recurrent upper respiratory tract infections and acute exacerbations in chronic obstructive pulmonary disease patients. OM-85 is able to potentiate both innate and adaptive immune responses. However, the molecular mechanisms responsible for OM-85 activation are still largely unknown. Purpose of this study was to investigate the impact of OM-85 stimulation on human dendritic cell functions. We show that OM-85 selectively induced NF-kB and MAPK activation in human DC with no detectable action on the interferon regulatory factor (IRF) pathway. As a consequence, chemokines (i.e. CXCL8, CXCL6, CCL3, CCL20, CCL22) and B-cell activating cytokines (i.e. IL-6, BAFF and IL-10) were strongly upregulated. OM-85 also synergized with the action of classical pro-inflammatory stimuli used at suboptimal concentrations. Peripheral blood mononuclear cells from patients with COPD, a pathological condition often associated with altered PRR expression pattern, fully retained the capability to respond to OM-85. These results provide new insights on the molecular mechanisms of OM-85 activation of the immune response and strengthen the rational for its use in clinical settings

Activation of PBMC and MoDC from COPD patients and healthy subjects by OM-85.

<p><b>A</b>) PBMC and <b>B</b>) MoDC (both 10⁶/ml) were stimulated as indicated in the presence or absence of 500 U/ml IFNγ or 100 ng/ml TNF-α. After 24 hours, supernatants were collected and analyzed by ELISA. Figure shows the results of healthy donors (open histograms) compared to COPD patients (black histograms). *P<0.05 by paired Student's <i>t</i> test.</p

Characteristic of COPD patients enrolled in the study.

<p>Table reports the main features (age, sex, age at diagnosis of COPD, disease stadium, therapy and notes) of selected patients <b>(P).</b></p

Induction of selected cytokines and chemokines by OM-85 in MoDC.

<p><b>A</b>) MoDC (10⁶/ml) were stimulated with OM-85 as indicated and with 100 ng/ml LPS (IL-6, BAFF, CCL2, CXCL8 and CXCL6) or 10 ng/ml LPS (CCL3, CCL20 and CCL22) as a positive control. After 24 hours, supernatants were collected and analyzed by ELISA. Ut = untreated. *P<0.05 and **P<0.01 by Dunnett's Multiple Comparison Test. <b>B</b>) Supernatants of MoDC stimulated with OM-85 induce a G-protein-dependent migration of PMN. As a comparison, migration of PMN was elicited with unstimulated supernatants+CXCL8 and PMA. As expected, migration to CXCL8 was inhibited by both 10 nM M3 and 750 ng/ml, <i>Pertussis toxin</i> (P.Tox) while migration to PMA was not. Results are expressed as chemotactic index over migration to supernatants of unstimulated MoDC and represent means+/−SD of three independent Boyden chamber experiments. *P value<0.05 and ** P value<0.01 by Dunnett's Multiple Comparison Test.</p

Activation of the NF-kB and MAPK pathways by OM-85 in MoDC.

<p><b>A</b>) Immature human MoDC were stimulated with 100 µg/ml OM-85 for 30, 60, 90 and 120 minutes. 100 ng/ml LPS was used as a positive control. After cell lysis and protein fractionation, cytoplasmic (Cyto) and nuclear (Nuclei) extracts were blotted against NF-kB p65 and IkBα. β-actin and Lamin A/C represent loading controls for cytoplasmic and nuclear proteins respectively. The image depicts results obtained in one representative donor out of eight. <b>B</b>) EMSA (upper panel) and supershift (lower panel) showing the induction of NFkBp65-DNA binding activity by OM-85 in human moDC stimulated as in A). Signal specificity was assessed by competing each sample with a 125-fold excess unlabeled probe (lanes 2,4,6,8,10 upper panel). The image depicts results obtained in one representative donor out of four. <b>C</b>) OM-85 induces the production of luciferase in THP1 cells bearing a NF-kB-reporter plasmid (NF-kB pGL4, striped histograms). THP1 cells were stimulated with 1 µg/ml LPS and 1000 µg/ml OM-85. As expected, THP1 untransfected cells (untransfected, empty histograms) did not produce luciferase in response to stimulation. Similar results were obtained when cells were transfected with the pGL4 empty backbone (pGL4, black histograms). Results are expressed as mean+/−SD of three independent experiments. *P value<0.01 by Dunnett's Multiple Comparison Test. <b>D</b>) OM-85 activates the MAPK pathway. Cell extracts prepared as in A) were blotted with antibodies specific for phophorilated ERK1/2 (Cyto, upper panel) and total ATF2 and c-Jun (Nuclei, lower panel). β-actin and Lamin A/C represent loading controls for cytoplasmic and nuclear proteins respectively. The image depicts results obtained in one representative donor out of three. <b>E</b>) OM-85 induces NF-kB- and MAPK-dependent gene transcription. Immature MoDC were stimulated with 100 µg/ml OM-85 (open circles) and 100 ng/ml LPS (black circles) for 2, 4, 8 and 24 hours. After RNA extraction, reverse transcription and DNAse I digestion, samples were amplified by Q-PCR using gene-specific primers. Results represent means+/−SE of three independent donors and are expressed as fold induction (FI) over unstimulated samples (0).</p

Synergism between OM-85 and pro-inflammatory agonists in MoDC.

<p>MoDC (10⁶/ml) were stimulated as indicated. After 24 hours, supernatants were collected and analyzed by ELISA. *P<0.05 and **P<0.005 by paired Student's <i>t</i> test.</p