Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach

Made available in DSpace on 2015-06-08T14:01:53Z (GMT). No. of bitstreams: 2 license.txt: 1914 bytes, checksum: 7d48279ffeed55da8dfe2f8e81f3b81f (MD5) anapaula_assefetal_IOC_2014.pdf: 1859957 bytes, checksum: 09c3b5c760f35acea9aafe7444daa579 (MD5) Previous issue date: 2014Fundação Oswaldo Cruz. Instituto de Tecnologia de Imunobiológicos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.undação Oswaldo Cruz. Instituto de Tecnologia de Imunobiológicos. Rio de Janeiro, RJ, Brasil.undação Oswaldo Cruz. Instituto de Tecnologia de Imunobiológicos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa de Infecções Hospitalares. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia de Imunobiológicos. Rio de Janeiro, RJ, Brasil.Purpose: Acinetobacter baumannii is an important opportunistic pathogen that causes pneumoniae, urinary tract infections, and/or septicemia in immunocompromised patients. This pathogen is frequently associated with nosocomial outbreaks worldwide and has become particularly problematic because of its prevalence and resistance patterns to several antibiotics. In the present study, we used an immunoproteome-based approach to identify immunogenic proteins located on the surface of A. baumannii for the development of a possible immunotherapy against this devastating bacterial infection. Experimental design: Sera from patients with A. baumannii infections (n = 50) and from a control group of healthy individuals (n = 3) were analyzed for reactivity against A. baumannii outer membrane proteins (OMPs) using Western blot analysis. To identify potential immunogenic proteins in A. baumannii,OMPs were separated by 2DE (2Delectrophoresis), and reactive sera from infected patients were randomly selected and divided into two different pools, each containing 15 sera. Finally, MALDI-TOF/TOF mass spectrometric analysis was employed to identify the corresponding proteins. Results: This analysis identified six immunoreactive proteins: OmpA, Omp34kDa, OprC, OprB-like, OXA-23, and ferric siderophore receptor protein. Notably, these proteins are highly abundant on the bacterial surface and involved in virulence, antibiotic resistance, and growth. Conclusions and clinical relevance: Our results support the notion that the proteins identified in the present immunoproteome study could serve as antigen candidates for the development of vaccines and passive immunotherapies against A. baumannii infections

O Brasil e o Conselho de Segurança da Organização das Nações Unidas: dos anos 90 a 2002 Brazil and the UN: struggle for a Role in the Security Council (From the Nineties to the Present Day)

A obtenção de um assento permanente no Conselho de Segurança da Organização das Nações Unidas tem sido uma aspiração constante da política externa brasileira desde o fim da Guerra Fria. Todavia, a ênfase interna na consecução de tal intento depende do êxito da aplicação da política econômica conjugado com alterações externas, como o atentado terrorista de setembro de 2001.<br>A permanent seat in the United Nations Security Council has been a constant aspiration of the Brazilian foreign policy since the end of the Cold War. However, the domestic emphasis in this endeavour depends on the success of the economic policy in association with international changes, provoked by events such as the September 11th