60 research outputs found
Rapid plant DNA and RNA extraction protocol using a bench drill
Plant DNA and RNA extraction methods are well established, with a wide range of protocols, depending on the purposes of each laboratory/research. Nowadays, quick, inexpensive and easy plant DNA and RNA extraction methods are highly sought after. We developed an optimized protocol for plant DNA and RNA extraction that uses an inexpensive bench drill and plastic bags and does not require liquid nitrogen. DNA from leaves and RNA from leaves and roots of banana, pineapple, citrus, papaya, passion fruit and cassava, were extracted using a basic cetyltrimethylammonium bromide method. Both nucleic acids were quantified and evaluated for quality based on agarose gel electrophoresis. The DNA and RNA extractions were successful for all species, and RNA quality in pellets was maintained after storage at room temperature for three weeks. This protocol can reduce costs considerably in laboratories with ongoing routine activities of DNA and RNA extraction for genetic diversity and gene expression analyses, where other conventional methods have not been successful due to explant, condition of samples and quantity and quality of nucleic acids. This is especially relevant for many laboratories in developing countries where the cost and availability of liquid nitrogen may be a constraint
Changes in vegetation cover of the Pantanal wetland detected by Vegetation Index: a strategy for conservation
Atributos físicos e químicos de substratos compostos por biossólidos e casca de arroz carbonizada
Low variation in ribosomal DNA and internal transcribed spacers of the symbiotic fungi of leaf-cutting ants (Attini: Formicidae)
Desenvolvimento ponderal e testicular, concentrações periféricas de testosterona e características de abate em touros da raça Nelore
Desenvolvimento vegetativo de pinhão-manso em diferentes arranjos de plantio em sistemas agrossilvipastoris
Manejo pré-abate de ovelhas de descarte: perdas de peso corporal, qualidade da carne e comportamento animal
Associação da medroxiprogesterona ao protocolo Ovsynch para inseminação artificial em tempo fixo de búfalas cíclicas (Bubalus bubalis) criadas na Amazônia Oriental
Reproductive parameters of Sindhi cows (Bos taurus indicus) treated with two ovulation synchronization protocols
Long-range Angular Correlations On The Near And Away Side In P-pb Collisions At √snn=5.02 Tev
7191/Mar294
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