IP3-4 kinase Arg1 regulates cell wall homeostasis and surface architecture to promote clearance of Cryptococcus neoformans infection in a mouse model

We previously identified a series of inositol polyphosphate kinases (IPKs), Arg1, Ipk1, Kcs1 and Asp1, in the opportunistic fungal pathogen Cryptococcus neoformans. Using gene deletion analysis, we characterized Arg1, Ipk1 and Kcs1 and showed that they act sequentially to convert IP3 to PP-IP5 (IP7), a key metabolite promoting stress tolerance, metabolic adaptation and fungal dissemination to the brain. We have now directly characterized the enzymatic activity of Arg1, demonstrating that it is a dual specificity (IP3/IP4) kinase producing IP5. We showed previously that IP5 is further phosphorylated by Ipk1 to produce IP6, which is a substrate for the synthesis of PP-IP5 by Kcs1. Phenotypic comparison of the arg1Δ and kcs1Δ deletion mutants (both PP-IP5-deficient) reveals that arg1Δ has the most deleterious phenotype: while PP-IP5 is essential for metabolic and stress adaptation in both mutant strains, PP-IP5 is dispensable for virulence-associated functions such as capsule production, cell wall organization, and normal N-linked mannosylation of the virulence factor, phospholipase B1, as these phenotypes were defective only in arg1Δ. The more deleterious arg1Δ phenotype correlated with a higher rate of arg1Δ phagocytosis by human peripheral blood monocytes and rapid arg1Δ clearance from lung in a mouse model. This observation is in contrast to kcs1Δ, which we previously reported establishes a chronic, confined lung infection. In summary, we show that Arg1 is the most crucial IPK for cryptococcal virulence, conveying PP-IP5-dependent and novel PP-IP5-independent functions

Dispersal of forest birds and trees along the Uruguay River in southern South America

The Uruguay River starts in Serra do Mar in Brazil, runs through the Paranense forest, and flows southward through grassland and savannas. It has a continuous gallery forest of 750 km from the southern border of the Paranense forest to the river mouth. The gallery forest extends for 100 km more along the Río de la Plata. 125 (68.7%) of the 182 species of forest birds recorded in the southern Paranense forest penetrate into the gallery forest of the Uruguay River and only 13 (7.1%) reach the end of the gallery forest (Punta Lara). The number of bird species is inversely correlated (r2 = 0.942) with distance and the slope of the regression is 58.10. This means a decline in diversity with 32% of species lost per unit distance. A hundred and eighty forest tree species were recorded in the southern Paranense forest, of which 113 (62.8%) penetrate into the gallery forest of the Uruguay River, and 28 (15.6%) reach Punta Lara. The number of tree species is inversely correlated (r2 = 0.976) with distance and the slope of the regression is -45.62. This means a decline in diversity with 25% of species lost per unit distance. The Uruguay River enables the dispersal of many species of forest birds and trees from the rain forest, but species richness tends to decrease with increased distance from the source area. A clear association pattern was found for birds between size, diet, habitat use and distance reached into the gallery forest. Species of smaller body size, granivores, insectivores and those that use both the interior and exterior parts of the gallery forest advanced noticeably further along the river than larger species, carnivores, nectarivores or frugivores, and those that frequent only a part of the forest. Similarly, a clear association between dispersal mechanism, water dependence and distance reached into the gallery forest was found for trees. Species with vegetative reproduction, zoochorous species and riparian species advanced markedly longer distances along the river than, anemochorous species and non-riparian species.Fil: Nores, Manuel Alberto. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Zoología Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cerana, María Micaela. Universidad Nacional de Córdoba; ArgentinaFil: Monserrat, Diego Andrés. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Zoología Aplicada; Argentin

Institutional shelter to protect adolescent victims of domestic violence: theory or practice?

OBJECTIVE: To understand and analyze, from the perspective of adolescent victims of domestic violence who were cared for in an institution in Campinas-SP, the protective factors to which they are submitted and / or have access. METHOD: This was qualitative research, with data collection occurring through focus groups with 17 adolescents, and semistructured interviews with seven of them; the data analysis was based on content analysis, using a thematic modality. RESULTS: Two themes emerged, entitled Four Walls and Trust. We discuss the context of institutional care, that despite the efforts made contemporaneously, still maintains an authoritarian environment; the importance of the bond and trust established with some employees, acting as protective factors for the adolescents and the protective aspect of religiosity. CONCLUSIONS: It is understood that these considerations should be valued and reinforced through the healthcare services provided to children and adolescents, as they contribute to the promotion of the physical and mental health of this population

Etnobotânica e medicina popular no tratamento de malária e males associados na comunidade ribeirinha Julião – baixo Rio Negro (Amazônia Central)

RESUMO A utilização de plantas medicinais para o tratamento de doenças tropicais como a malária na Amazônia Central é de suma importância, principalmente em locais onde o sistema único de saúde não se encontra presente como na maioria das comunidades ribeirinhas desta região. Sendo assim, investigar e resgatar o conhecimento popular a respeito de plantas medicinais utilizadas no tratamento de malária e males associados pelos moradores da comunidade Julião situada na Reserva de Desenvolvimento Sustentável do Tupé, Manaus-AM, torna-se importante no registro de como as populações locais se previnem e tratam essa doença tão prevalente e perigosa na região. O trabalho foi conduzido na forma de oficinas participativas, segregadas por gênero e complementadas com entrevistas semiestruturadas aliadas à técnica da turnê-guiada nos quintais e floresta adjacente à comunidade. Foram calculados os índices de diversidade de Shannon-Wiener, equitabilidade e concordância quanto ao uso principal (CUP). A partir da colaboração efetiva de 13 comunitários foram registradas 62 espécies vegetais pertencentes a 53 gêneros e 34 famílias botânicas que resultaram em índice de diversidade (H’) de 1,62 decits e equitabilidade de 0,9. As famílias mais representativas foram: Fabaceae (7 espécies), Asteraceae e Lamiaceae (4 espécies cada) e Solanaceae e Rutaceae (3 espécies cada). Vale destacar que 16 espécies (25,8%) foram citadas para tratamento de malária e males associados pela primeira vez em estudos etnobotânicos realizados na América Latina