Avaliação imunoistoquímica dos componentes fibrilares na matriz extracelular da fáscia transversal e da bainha do músculo reto abdominal de homens portadores de hérnia inguinal

OBJECTIVE: to evaluate the role of fibrillar extracellular matrix components in the pathogenesis of inguinal hernias. METHODS: samples of the transverse fascia and of the anterior sheath of the rectus abdominis muscle were collected from 40 men aged between 20 and 60 years with type II and IIIA Nyhus inguinal hernia and from 10 fresh male cadavers (controls) without hernia in the same age range. The staining technique was immunohistochemistry for collagen I, collagen III and elastic fibers; quantification of fibrillar components was performed with an image analysis processing software. RESULTS: no statistically significant differences were found in the amount of elastic fibers, collagen I and collagen III, and the ratio of collagen I / III among patients with inguinal hernia when compared with subjects without hernia. CONCLUSION: the amount of fibrillar extracellular matrix components did not change in patients with and without inguinal hernia.OBJETIVO: avaliar a participação dos componentes fibrilares da matriz extracelular na etiopatogenia das hérnias inguinais. MÉTODOS:foram retiradas amostras da fáscia transversal e da bainha anterior do músculo reto abdominal de 40 homens na faixa etária entre 20 e 60 anos, portadores de hérnia inguinal tipo II e IIIA de Nyhus e de 10 controles constituído por cadáveres frescos, na mesma faixa etária, sem hérnia. A técnica de coloração foi a imunoistoquímica para colágeno I, colágeno III e fibras elásticas e a quantificação dos componentes fibrilares foi realizada através de sistema computadorizado e software de processamento e análise de imagem. RESULTADOS: não foram encontradas diferenças estatisticamente significantes na quantidade de fibras elásticas, colágeno I e colágeno III, e na proporção de colágeno I/III entre os pacientes portadores de hérnia inguinal em comparação com indivíduos sem hérnia. CONCLUSÃO:a quantidade dos componentes fibrilares da matriz extracelular não se alterou nos pacientes com e sem hérnia inguinal.UnifoaUNIFESP-EPMUNIFESP, EPMSciEL

Study histochemistry quantitative of the proteoglycans of the extracellular matrix of the transversal fascia and anterior sheath of the rectus abdominal muscle in males adults, with inguinal hernia type II and IIIA of Nyhus

BV UNIFESP: Teses e dissertaçõe

Biochemic comparative study of the glysosaminoglycans dermatan sulfate and condroitin sulfate of the matrix extracelular of the tranversal fascia and anterior rectus sheat abdominal muscle in males adults, with inguinal hernia type II of Nyhus

Objectives: To compare the quantities of the glycosaminoglycans dermatan sulfate and chondroitin sulfate between male patients with Nyhus type II inguinal hernias and individuals without inguinal hernias, aged between 20 and 40 years. Method: Two groups were formed: one comprising 15 male patients aged 20 to 40 years with Nyhus type II inguinal hernias and ASA risk I and II; and another (controls) comprising 10 male individuals aged 20 to 40 years without inguinal hernias, who died within 24 hours. Female patients, diabetics, individuals with connective tissue disease, smokers and patients with ASA surgical risk III and IV were excluded. Samples (1 cm2) were taken from the transversalis fascia in the intermediate part of the inguinal triangle and from the anterior sheath of the rectus abdominis muscle in the corresponding inguinal region. Dermatan sulfate and chondroitin sulfate were quantified using densitometry after electrophoresis on agarose gel, on the transversalis fascia and the anterior sheath of the rectus abdominis muscle, between the groups with and without hernias and between the groups separately. The Wilcoxon, Mann-Whitney and Student t tests were used for the statistical analysis. For all tests, the significance level was alpha < 0.05 or 5%. Results: The quantities of dermatan sulfate and chondroitin sulfate did not present any statistically significant difference between the patients with inguinal hernias and the individuals without inguinal hernias, either in the transversalis fascia or in the anterior sheath of the rectus abdominis muscle. The percentage of dermatan sulfate was greater than the quantity of chondroitin sulfate, both in the transversalis fascia and in the anterior sheath of the rectus abdominis muscle. This result was statistically significant. Conclusion: There was no difference in quantities of dermatan sulfate and chondroitin sulfate between the patients with Nyhus type II inguinal hernias and the individuals without inguinal hernias, aged between 20 and 40 yearsObjetivo: Comparar a quantidade de glicosaminoglicanos dermatam sulfato e condroitim sulfato entre pacientes homens, portadores de hernia inguinal tipo II de Nyhus e individuos sem hernia inguinal, com idade entre 20 e 40 anos. Metodos: Foram constituidos dois grupos. Um grupo de 15 pacientes do sexo masculino, com hernia inguinal tipo II de Nyhus, com idade entre 20 e 40 anos, com risco ASA I e II. Um grupo controle com 10 individuos, tambem do sexo masculino, com morte ate 24 horas, com idade entre 20 e 40 anos. Foram excluidos os pacientes do sexo feminino, diabeticos, portadores de doenca do tecido conjuntivo, tabagistas e com risco cirurgico ASA III e IV. Foi retirada uma amostra de 1cm2 da fascia transversal na parte intermediaria do trigono inguinal e 1cm2 na bainha anterior do musculo reto abdominal na regiao inguinal correspondente e quantificados os glicosaminoglicanos dermatam sulfato e condroitim sulfato, por densitometria, apos eletroforese em gel de agarose.na fascia transversal e bainha anterior do musculo reto abdominal entre os grupos com hernia e sem hernia e, entre os grupos separadamente. Para analise estatistica foram utilizados os testes de Wilcoxon, Mann-Whitney e Student. Para todos os testes o nivel de significancia foi de alfa. 0,05 ou de 5%. Resultados: A quantidade de dermatam sulfato nao apresentou diferenca estatisticamente significante entre os pacientes com hernia inguinal e os individuos sem hernia inguinal, tanto na fascia transversal (p valor=0,108) quanto na bainha anterior do musculo reto abdominal (p valor=0,292). A quantidade de condroitim sulfato nao apresentou diferenca estatisticamente significante entre os pacientes com hernia inguinal e os individuos sem hernia inguinal, tanto na fascia transversal (p valor=0,579) quanto na bainha anterior do musculo reto abdominal (p valor=0,0,180). A porcentagem do glicosaminoglicano dermatam sulfato foi maior que a quantidade de condroitim sulfato tanto na fascia transversal quanto na bainha anterior do musculo reto abdominal. Este resultado foi estatisticamente significante. Conclusao: Nao verificamos diferenca na quantidade de glicosaminoglicanos dermatam sulfato e condroitim sulfato entre os pacientes portadores de hernia inguinal tipo II de Nyhus e individuos sem hernia inguinal, em homens adultos, com idade entre 20 e 40 anos.TEDEBV UNIFESP: Teses e dissertaçõe

Time-dependent effect of refrigeration on viscosity and conversion kinetics of dental adhesive resins

Objectives: This study evaluated the effect of refrigeration at 4oC and post-refrigeration times (immediate, 5, 10, 15, or 20 min) on the viscosity and conversion kinetics of adhesive bonding resins. Methods: Scotchbond Dual-Cure (3M ESPE) and Clearfil SE Bond (Kuraray) were tested. Control samples were kept at 25oC for 24 h. At each post-refrigeration time, the temperature was checked with a K-type thermocouple. Viscosity measurements as a function of temperature were performed using a cone-plate viscometer. Real-time polymerization was monitored by infrared spectroscopy. Degree of conversion (DC) was calculated for each second during polymerization, and the rate of polymerization analyzed. Data were separately submitted to two-way ANOVA and Tukey’s test (P<.05). Results: Clearfil presented faster increase in temperature after exposure to room temperature than Scotchbond. A continuous decrease in viscosity (Pa.s) was observed for both Scotchbond (0.49, 0.34, 0.30, 0.26, 0.23, 0.23) and Clearfil (0.38, 0.37, 0.34, 0.25, 0.24, 0.22). For Scotchbond, higher final DC was detected for the control (62.7%) compared with the immediate (53.3%) and 5 min (54.7%) groups. For Clearfil, the control sample (81.4%) showed higher DC than all refrigerated groups (68.8– 69.5%). Clearfil always showed significantly higher DC than Scotchbond. Conclusions: Refrigeration presented a significant time- and material-dependent effect on the viscosity and polymerization kinetics of the bonding resins. Under clinical conditions, adhesive agents should be removed from the refrigerator at least 20 min before being used. (Eur J Dent 2010;4:150-155