Elis Regina em Black is Beautiful (1970), de Marcos Valle e Paulo Sergio Valle

Mapeamento de efeitos vocais e análise das relações entre a letra, a música e as expressões corporais na performance de Black is Beautiful (1971), dos compositores Paulo Sergio Valle (1940) e Marcos Valle (1943), realizada em 1972 pela cantora Elis Regina (1945-1982) no 1 Plus, um especial da TV alemã Südwestfunks em parceria com a Globo. Este estudo, que recorre ao mAAVm (Método de Análise de Áudios e Vídeos de Música) e suas ferramentas, descreve e compara ocorrências de sentidos de palavras no poema (semânticos e conotações afetivas), efeitos vocais em espectrogramas sonoros de oito efeitos vocais (portamento, vibrato, scoop, yodel, drive, crepitação, modulação timbrística e respiração ruidosa) e gestos (faciais, cabeça, tronco e braços). Os resultados revelam que a intérprete construía suas performances com base em um planejamento minucioso, criando ênfases de sincronia e estabelecendo claras relações no trinômio formado por texto (ou contexto), sons e gestual

Autonomic fiber sprouting in the skin in chronic inflammation

Pain is a major symptom associated with chronic inflammation. In previous work from our laboratory, we have shown that in animal models of neuropathic pain there is a sprouting of sympathetic fibers into the upper dermis, a territory normally devoid of them. However, it is not known whether such sympathetic spouting, which is likely trophic factor mediated, also occurs in chronic inflammation and arthritis. In the present study, we used a rat model of chronic inflammation in which a small single dose of complete Freund's adjuvant (CFA) was injected subcutaneously, unilaterally, into the plantar surface of the hindpaw. This led to a localized long-term skin inflammation and arthritis in all joints of the hindpaw. Animals were perfused with histological fixatives at 1, 2, 3 or 4 weeks after the injection. Experimental animals treated with CFA were compared to saline-injected animals. We then investigated the changes in the pattern of peripheral innervation of the peptidergic nociceptors and sympathetic fibers in rat glabrous hindpaw skin. Antibodies directed towards calcitonin gene-related peptide (CGRP) and dopamine beta-hydroxylase (DBH) were used for the staining of peptidergic and sympathetic fibers, respectively. Immunofluorescence was then used to analyze the different nerve fiber populations of the upper dermis. At 4 weeks following CFA treatment, DBH-immunoreactive (IR) fibers were found to sprout into the upper dermis, in a pattern similar to the one we had observed in animals with a chronic constriction injury of the sciatic nerve in a previous publication. There was also a significant increase in the density of CGRP-IR fibers in the upper dermis in CFA treated animals at 2, 3 and 4 weeks post-injection. The increased peptidergic fiber innervation and the ectopic autonomic fibers found in the upper dermis may have a role in the pain-related behavior displayed by these animals

Distinctive response of CNS glial cells in oro-facial pain associated with injury, infection and inflammation

Oro-facial pain following injury and infection is frequently observed in dental clinics. While neuropathic pain evoked by injury associated with nerve lesion has an involvement of glia/immune cells, inflammatory hyperalgesia has an exaggerated sensitization mediated by local and circulating immune mediators. To better understand the contribution of central nervous system (CNS) glial cells in these different pathological conditions, in this study we sought to characterize functional phenotypes of glial cells in response to trigeminal nerve injury (loose ligation of the mental branch), infection (subcutaneous injection of lipopolysaccharide-LPS) and to sterile inflammation (subcutaneous injection of complete Freund's adjuvant-CFA) on the lower lip. Each of the three insults triggered a specific pattern of mechanical allodynia. In parallel with changes in sensory response, CNS glial cells reacted distinctively to the challenges. Following ligation of the mental nerve, both microglia and astrocytes in the trigeminal nuclear complex were highly activated, more prominent in the principal sensory nucleus (Pr5) and subnucleus caudalis (Sp5C) area. Microglial response was initiated early (days 3-14), followed by delayed astrocytes activation (days 7-28). Although the temporal profile of microglial and astrocyte reaction corresponded respectively to the initiation and chronic stage of neuropathic pain, these activated glial cells exhibited a low profile of cytokine expression. Local injection of LPS in the lower lip skin also triggered a microglial reaction in the brain, which started in the circumventricular organs (CVOs) at 5 hours post-injection and diffused progressively into the brain parenchyma at 48 hours. This LPS-induced microglial reaction was accompanied by a robust induction of IκB-α mRNA and pro-inflammatory cytokines within the CVOs. However, LPS induced microglial activation did not specifically occur along the pain signaling pathway. In contrast, CFA injection led to minor microglial morphological changes and an induction of IκB-α mRNA in the CVO regions; a significant increase in IL-1β and IL-6 mRNA started only at 48 hours post-injection, when the induced pain-related behavior started to resolve. Our detailed analysis of CNS glial response clearly revealed that both nerve injury and oro-facial infection/inflammation induced CNS glial activation, but in a completely different pattern, which suggests a remarkable plasticity of glial cells in response to dynamic changes in their microenvironment and different potential involvement of this non-neuronal cell population in pathological pain development

Developing a Plastic Waste Management Program: From River Basins to Urban Beaches (Case Study)

Solid waste accumulation in coastal environments has been a growing concern. In the coastal megacity of Recife, Pernambuco state, Brazil, plastic waste issues currently exist both at the ocean scale and river basin scale. The city is known as the 'Brazilian Venice' thanks to the Capibaribe River, which crosses many neighborhoods, running in a west-east direction into the Atlantic Ocean. This paper provides the initial basis to develop a Plastic Waste Management Program proposal for implementation in the city of Recife, given the lack of resolutions that have looked at plastic waste management through integrated water environment scales (from river basin to ocean). The methodology used included articulation and documental collection from four main public agencies from the state/city and stakeholders. The results showed its relevance for better plastic waste management in Recife, considering an integrated water environment on a river basin-to-ocean scale by using the recognized major connected water environments (Capibaribe river and Boa Viagem beach). Similar integrated program proposals could be made for other coastal areas, enabling not only the identification of fragilities but also the exchange of information regarding the theme

Análise da Sazonalidade em uma Academia de Ginástica de Florianópolis, Santa Catarina

As constantes oscilações no número de clientes de academias de ginástica e outros centros esportivos, ao longo dos anos, são, normalmente, motivo de preocupação para grande parte dos gestores responsáveis em manter os empreendimentos lucrativos. Estas variações do número de clientes – comumente produto de comportamentos sazonais da clientela – podem influenciar significativamente nos resultados financeiros de uma organização e seu entendimento pode originar indicadores para auxiliar na gestão das organizações. Calcado nesta perspectiva, o objetivo deste estudo foi identificar e descrever as variações sazonais enfrentadas por uma academia de ginástica situada na região central da cidade de Florianópolis, Santa Catarina, Brasil. Os dados foram coletados dos arquivos de dados da organização pesquisada e refletem a variação do número de clientes da empresa durante o período 2007 a 2011. Através dos resultados, pôde-se identificar que a variação do número de clientes da academia de ginástica tende a apresentar um comportamento bem definido ao longo do período analisado. Observou-se uma tendência de crescimento do número de clientes ao longo dos trimestres do ano. Conclui-se que, além dos fatores climáticos, os fatores culturais/sociais são importantes influenciadores da sazonalidade em uma academia de ginástica

Beta 1 integrin predicts survival in breast cancer: a clinicopathological and immunohistochemical study

Abstract\ud \ud \ud \ud Background\ud \ud The main focus of several studies concerned with cancer progression and metastasis is to analyze the mechanisms that allow cancer cells to interact and quickly adapt with their environment. Integrins, a family of transmembrane glycoproteins, play a major role in invasive and metastatic processes. Integrins are involved in cell adhesion in both cell-extracellular matrix and cell-cell interactions, and particularly, β1 integrin is involved in proliferation and differentiation of cells in the development of epithelial tissues. This work aimed to investigate the putative role of β1 integrin expression on survival and metastasis in patients with breast invasive ductal carcinoma (IDC). In addition, we compared the expression of β1 integrin in patients with ductal carcinoma in situ (DCIS).\ud \ud \ud \ud Methods\ud \ud Through tissue microarray (TMA) slides containing 225 samples of IDC and 67 samples of DCIS, β1 integrin expression was related with several immunohistochemical markers and clinicopathologic features of prognostic significance.\ud \ud \ud \ud Results\ud \ud β1 integrin was overexpressed in 32.8% of IDC. In IDC, β1 integrin was related with HER-2 (p = 0.019) and VEGF (p = 0.011) expression and it had a significant relationship with metastasis and death (p = 0.001 and p = 0.05, respectively). Kaplan-Meier survival analysis showed that the overexpression of this protein is very significant (p = 0.002) in specific survival (number of months between diagnosis and death caused by the disease). There were no correlation between IDC and DCIS (p = 0.559) regarding β1 integrin expression.\ud \ud \ud \ud Conclusions\ud \ud Considering that the expression of β1 integrin in breast cancer remains controversial, specially its relation with survival of patients, our findings provide further evidence that β1 integrin can be a marker of poor prognosis in breast cancer.\ud \ud \ud \ud Virtual slides\ud \ud The virtual slide(s) for this article can be found here: \ud http://www.diagnosticpathology.diagnomx.eu/vs/6652215267393871We thank CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil) and FAPESP (Fundação de Amparo a Pesquisa do Estado de São Paulo, Brazil) for financial support, CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil) and FACEPE (Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco, Brazil) for schollarships and financial support; and Deisy Mara da Silva for technical assistance.This research is in accordance with Declaration of Helsinki, and was aprooved by the local ethics comitee

Keratinocyte growth factor, tumor necrosis factor-alpha and interleukin-1 beta gene expression in cultured fibroblasts and keratinocytes from burned patients

PURPOSE: To evaluate the gene expression of KGF, TNF-alpha and IL-1 beta in skin fibroblasts and keratinocytes cultured from burned patients. METHODS: Three patients with large burns and three patients with small burns, as well as two controls, were included. The cell culture was initiated by the enzymatic method. After extraction and purification of mRNA, qPCR was used to assess the gene expression of KGF, TNF-alpha and IL-1 beta. RESULTS: The expression of KGF was increased on average 220-fold in large burns and 33.33-fold in small burns in fibroblasts, and 11.2-fold in large burns and 3.45-fold in small burns in keratinocytes compared to healthy patients (p<0.05). Expression of TNF-alpha was not observed. IL-1 beta is down-regulated in fibroblasts of burned patients, and much more repressed in small burns (687-fold, p<0.05). In keratinocytes, the repression of IL-1 beta expression occurs in patients with small burns (28-fold), while patients with large burns express this gene intensively (15-fold). CONCLUSIONS: The study showed a quantitative pattern in the expression of KGF gene, which is more expressed according to the size of the burn. TNF-alpha was not expressed. A qualitative pattern in the expression of IL-1 beta gene was demonstrated.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)UNIFESP-EPM Department of SurgeryUNIFESP-EPMUNIFESP-EPM Department of GynecologyUNIFESP, EPM, Department of SurgeryUNIFESP-EPMUNIFESP, EPM Department of GynecologySciEL