Avaliação de metodologia para análise de íons por cromatografia iônica em água de nascentes.

Editores técnicos: Marcílio José Thomazini, Elenice Fritzsons, Patrícia Raquel Silva, Guilherme Schnell e Schuhli, Denise Jeton Cardoso, Luziane Franciscon. EVINCI. Resumos

Development of an educational software for the design of solid-liquid separation in tubular centrifuges

A tecnologia está cada dia mais presente na vida moderna e tem sido importante aliada no ensino-aprendizagem dos cursos superiores de tecnología e ciencias exatas, principalmente nas engenharias. Muitas vezes, os discentes, julgam como difícil de asimilar conceitos abstratos, debido à grande quantidade de teoria e cálculos envolvidos dificultando a compreensão. A fim de reverter essa visão, o artigo tem como objetivo demonstrar a utilização da tecnologia no âmbito educacional e contribuir nas melhorias da aprendizagem dos conteúdos obrigatórios de um curso superior em engenharia química. Para tal, idealizou o “Projeto Centrífuga”, como ferramenta alternativa ao ensino de projeto de centrifugas tubulares, através de dados das condições operacionais e ampliação de escalas de projetos. Concluiu-se que a tecnologia é uma importante aliada na no proceso educacional incentivando os acadêmicos tornando-os parte da construção do conhecimento.Technology is increasingly present in modern life and has been an important ally in the teaching and learning of advanced courses in technology and the exact sciences, especially in engineering. Often, the students think it difficult to assimilate abstract concepts, due to the large amount of theory and calculations involved making it difficult to understand. In order to reverse this view, the article aims to demonstrate the use of technology in the educational field and to contribute to improvements in the learning of the compulsory contents of a course in chemical engineering. For this purpose, he designed the "Centrifugal Project", as an alternative tool to teaching tubular centrifugal design, through data of the operational conditions and expansion of project scales. It was concluded that technology is an important ally in the educational process by encouraging academics to become part of the construction of knowledge.Facultad de Informátic

The effect of salts on the liquid–liquid phase equilibria of PEG600 + salt aqueous two-phase systems

Six new ATPSs were prepared by combining polyethylene glycol PEG600 with potassium citrate, dipotassium hydrogen phosphate, sodium formate, potassium formate, sodium sulfate, and lithium sulfate. Complete phase diagrams, including the binodal curve and three tie-lines, were determined at 23 °C. The experimental data obtained for the binodal curve were successfully adjusted to the Merchuk equation, and the reliability of tie-line data was confirmed using the equations suggested by Othmer–Tobias and Bancroft. The ability of each ion to induce ATPS formation was investigated. Na+ proved to be more effective in ATPS formation than K+ and Li+. For potassium salts, the order observed for the effectiveness of the anions was: HPO42– > C6H5O73– > HCO2–. Regarding the sodium salts, it was found that SO42– is clearly more effective than HCO2–. The position of the ions in the Hofmeister series and their free energy of hydration (ΔGhyd) were used to explain the ability of the ions to induce PEG salting-out. Furthermore, the effective excluded volume (EEV) of the salts was determined and the following order was found: Na2SO4 > K2HPO4 > Li2SO4 > K3C6H5O7 > NaCHO2 > KCHO2. Similar order was obtained when analyzing the size of the heterogeneous regions, suggesting the practical use of EEV as a comparison parameter between different ATPSs.This work is partially supported by project PEst-C/EQB/LA0020/2011, financed by FEDER through COMPETE-Programa Operacional Factores de Competitividade and by FCT-Fundacao para a Ciencia e a Tecnologia. Sara Silverio acknowledges her Ph.D. grant from FCT (SFRH/BD/43439/2008)

Serum amyloid A proteins reduce bone mass during mycobacterial infections

IntroductionOsteopenia has been associated to several inflammatory conditions, including mycobacterial infections. How mycobacteria cause bone loss remains elusive, but direct bone infection may not be required. MethodsGenetically engineered mice and morphometric, transcriptomic, and functional analyses were used. Additionally, inflammatory mediators and bone turnover markers were measured in the serum of healthy controls, individuals with latent tuberculosis and patients with active tuberculosis. Results and discussionWe found that infection with Mycobacterium avium impacts bone turnover by decreasing bone formation and increasing bone resorption, in an IFN gamma- and TNF alpha-dependent manner. IFN gamma produced during infection enhanced macrophage TNF alpha secretion, which in turn increased the production of serum amyloid A (SAA) 3. Saa3 expression was upregulated in the bone of both M. avium- and M. tuberculosis-infected mice and SAA1 and 2 proteins (that share a high homology with murine SAA3 protein) were increased in the serum of patients with active tuberculosis. Furthermore, the increased SAA levels seen in active tuberculosis patients correlated with altered serum bone turnover markers. Additionally, human SAA proteins impaired bone matrix deposition and increased osteoclastogenesis in vitro. Overall, we report a novel crosstalk between the cytokine-SAA network operating in macrophages and bone homeostasis. These findings contribute to a better understanding of the mechanisms of bone loss during infection and open the way to pharmacological intervention. Additionally, our data and disclose SAA proteins as potential biomarkers of bone loss during infection by mycobacteria.This article is a result of the project HEALTH-UNORTE: Setting-up biobanks and regenerative medicine strategies to boost research in cardiovascular, musculoskeletal, neurological, oncological, immunological and infectious diseases (NORTE-01-0145-FEDER-000039), supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). This work was supported by KOG-202108-00929 from the European Haematology Society, awarded to AG. Work in the MS lab was financed by FEDER - Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020 - Operacional Programme for Competitiveness and Internationalisation (POCI), Portugal 2020, and by Portuguese funds through FCT - Fundacao para a Ciencia e a Tecnologia/Ministerio da Ciencia, Tecnologia e Ensino Superior in the framework of the project POCI-01-0145-FEDER-028955 (PTDC/SAU-INF/28955/2017). AG and MS are supported by an Individual Scientific Employment contract (CEECIND/00048/2017; CEECIND/00241/2017 respectively). DS acknowledges the Portuguese Foundation for Science and Technology (FCT) for the Post-Doc fellowship (SFRH/BPD/115341/2016). RP, DS and AF have PhD grants (SFRH/BD/145217/2019; SFRH/BD/143536/2019; 2020.05949.BD, respectively) financed by FCT

Reparo da luz da narina direita e oclusão de fístula oronasal decorrente de miíase em um cão (Canis familiaris)

O artigo não apresenta resumo

Aplicação de marcadores moleculares na conservação e melhoramento de um rebanho da raça Santa Inês.

0 objetivo deste trabalho foi caracterizar a distribuiçao da variabilidade genetica dentro de um Nucleo de Conservaçao da raça Santa Ines a partir do usa de marcadores moleculares. Foram utilizados 17 locos em 191 individuos do rebanho de Santa Ines da Embrapa Tabuleiros Costeiros, Sergipe. Os resultados obtidos mostraram que existe variabilidade genetica significativa existente dentro do nucleo com valores de Heterezigosidade media observada de 0,702 e de conteudo de informaçao polimorfica (PIC) de 0,704. Outras analises intrapopulacionais mostraram que 29% dos locos analisados nao estao em Equilfbrio de Hardy-Weinberg (p<0,01) e apresentaram excesso de homozigotos (p<0,05). A partir do fndice de proporçao de alelos compartilhados entre todos os individuos analisados, foi feito um dendrograma para estimar quais seriam os animais mais proximos ou mais distantes dentro do rebanho, com especial atençao para os 10 machos reprodutores. Tres agrupamentos principais foram identificados e em todos existe pelo menos um reprodutor do rebanho. Esse tipo de analise e importante, pais ira auxiliar o manejo reprodutivo do rebanho bem com servira de ferramenta complementar para os programas de melhoramento classico que se iniciaram dentro deste Nucleo de Conservaçao

Morte encefálica e cuidados na manutenção do potencial doador de órgãos e tecidos para transplante

Este trabalho trata-se de estudo exploratório-descritivo com abordagem quantitativa e dados prospectivos que objetivou verificar o conhecimento dos profissionais de enfermagem sobre a Morte Encefálica (ME) e a manutenção do Potencial Doador (PD). A população constou de 55 profissionais de enfermagem. Destes, a maioria eram técnicos em enfermagem (74,5%); 78,2% informaram já ter trabalhado com PDs e 50,9% afirmaram estar preparados para cuidar desses pacientes. Das condições indispensáveis para a abertura do protocolo de ME, 49,1% afirmaram erroneamente a temperatura superior a 36ºC. No manejo dos distúrbios hidroeletrolíticos, 50,9% optaram incorretamente sobre a reposição de sódio, potássio e magnésio. Nos cuidados com córneas, 58,2% optaram erradamente sobre a proteção com gaze. E 52,7% afirmaram corretamente que o PD pode ser reanimado. O conhecimento sobre o diagnóstico de ME e manutenção ao PD era insuficiente entre os pesquisados, necessitando de educação sobre o tema a fim de aumentar a oferta de órgãos/tecidos para transplantes. Descritores: Enfermagem; Morte Encefálica; Transplante; Unidade de Terapia Intensiva