Pengaruh Pencucian Sperma dengan Lama Waktu Sentrifugasi yang Berbeda Terhadap Kualitas Sperma Kambing Bligon (Effect of Sperm Washing with Different Centrifugation Duration on Sperm Quality of Bligon Buck)

The objective of the experiment was to examine the effect of sperm washing using SpermRinse with different centrifugation duration (15, 20, and 25 minutes) on the quality of Bligon spermatozoa. Sperm was collected using artificial vagina from six bucks of 2 (two) year olds. Data were collected for motility, viability, and abnormality of the spermatozoa. Characteristics of the fresh sperm were analyzed based on mean and deviation standard, and followed by Duncan’s New Multiple Range Test (DMRT). The results showed that washing Bligon buck sperm using SpermRinse with different centrifugation times had very significant effect (P≤0.01) on the motility, viability, and abnormality of spermatozoa. It is concluded that washing Bligon buck sperm using SpermRinse with centrifugation at 1.500 rpm (r = 5.5 cm) could increased sperm quality, of which 20 minutes was the best centrifugation time. (Key word: Bligon Buck, Sperm, SpermRinse, Sperm quality

Stimulasi Laser sebagai Alternatif untuk Induksi Estrus pada Kambing Bligon (Laser Stimulation as an Alternative for Estrus Induction on Bligon Goats)

Reproduction is a very important factor in maintaining livestock productivity. For better reproductivity, estrus induction is one of very important ways to be performed. Estrus induction is usually performed with hormonal method,such as application of PGF2α, but the disadvantage is that it is relatively expensive. For this reason, it is necessary to promote technological breakthrough to lower cost of estrus induction. The aim of this study was to know whether laserstimulation on the points of reproductive acupuncture could be performed for estrus induction on Bligon goats, as it is usually performed with hormonal method. Fourteen female Bligon goats aged 2 years old were randomly divided into two groups, each group consisted of 7 goats. Group I was stimulated with laser and group II was injected with 1.25 mg per head of PGF2α. Laser stimulation was conducted for 10 seconds on each point of acupuncture on day 1, 2, and 3, and was repeated on day 11, 12, and 13. PGF2α injection was performed on day 1 and was repeated on day 11. The observed variables were the percentage of estrus, onset of estrus, estrus duration and progesterone level when estrus occurred after the second estrus induction. The results of the study showed that laser stimulation for estrus induction did not indicate significant difference compared to PGF2α injection, in terms of the percentage of goats undergoingestrus, onset of estrus and the estrus duration. It was concluded that laser stimulation could be used for estrus induction on Bligon goats.(Key words: Laser, Estrus induction, Bligon goats

Pengaruh Pencucian Sperma dengan Lama Waktu Sentrifugasi yang Berbeda Terhadap Kualitas Sperma Kambing Bligon (Effect of Sperm Washing with Different Centrifugation Duration on Sperm Quality of Bligon Buck)

The objective of the experiment was to examine the effect of sperm washing using SpermRinse with different centrifugation duration (15, 20, and 25 minutes) on the quality of Bligon spermatozoa. Sperm was collected usingartificial vagina from six bucks of 2 (two) year olds. Data were collected for motility, viability, and abnormality of the spermatozoa. Characteristics of the fresh sperm were analyzed based on mean and deviation standard, and followed by Duncan’s New Multiple Range Test (DMRT). The results showed that washing Bligon buck sperm using SpermRinse with different centrifugation times had very significant effect (P≤0.01) on the motility, viability, and abnormality ofspermatozoa. It is concluded that washing Bligon buck sperm using SpermRinse with centrifugation at 1.500 rpm (r = 5.5 cm) could increased sperm quality, of which 20 minutes was the best centrifugation time.(Key word: Bligon Buck, Sperm, SpermRinse, Sperm quality

PRE WEANING GROWTH OF BALI CALVES AT BALAI PEMBIBITAN TERNAK UNGGUL SAPI BALI

Balai Pembibitan Ternak Unggul is a breeding center established by the Government of Indonesia (GOI) to perform preservation, breeding, breeding stock production and development, and also the distribution of breeding stock of Excellent Bali cattle at national level. In the production of excellent breeding stock through the performance test and progeny test. Pre-weaning growth is one of the important indicators for the population growth. The study was aimed to identify the pre-weaning calf growth rate of Bali cattle in 2010-2011, born from performance-test-passing-dams that were collectively reared with progeny-test-passing sires. Materials used were 84 pre-weaning Bali calves. This was a survey method-based descriptive analysis research. The results showed that in terms of the performance of pre-weaning growth, the birth weights of male and female calves were 18.37±1.65 kg and 18.27±1.29 kg, respectively. The weaning weights of male and female calves were 93.53±21.00 kg and 87.66±12.04 kg, respectively, with weaning time of 205 days. The average of daily weight gain (ADG) were 0.37±0.10 kg/head/day (male) and 0,34±0,06 kg/head/day (female). It was concluded that the pre-weaning calf growth rate of Bali cattle at the BPTU Bali in 2010-2011 were moderately high since they were born from the dams and the sires passing the performance test and progeny test.(Keywords: Pre-weaning growth, Bali cattle)Balai Pembibitan Ternak Unggul is a breeding center established by the Government of Indonesia (GOI) to perform preservation, breeding, breeding stock production and development, and also the distribution of breeding stock of Excellent Bali cattle at national level. In the production of excellent breeding stock through the performance test and progeny test. Pre-weaning growth is one of the important indicators for the population growth. The study was aimed to identify the pre-weaning calf growth rate of Bali cattle in 2010-2011, born from performance-test-passing-dams that were collectively reared with progeny-test-passing sires. Materials used were 84 pre-weaning Bali calves. This was a survey method-based descriptive analysis research. The results showed that in terms of the performance of pre-weaning growth, the birth weights of male and female calves were 18.37±1.65 kg and 18.27±1.29 kg, respectively. The weaning weights of male and female calves were 93.53±21.00 kg and 87.66±12.04 kg, respectively, with weaning time of 205 days. The average of daily weight gain (ADG) were 0.37±0.10 kg/head/day (male) and 0,34±0,06 kg/head/day (female). It was concluded that the pre-weaning calf growth rate of Bali cattle at the BPTU Bali in 2010-2011 were moderately high since they were born from the dams and the sires passing the performance test and progeny test.(Keywords: Pre-weaning growth, Bali cattle

The Effect of Thawing Duration on the Post Thawing Quality of Bali Cattle’s Frozen Semen and Conseption Rate in Smallholder Farms of East Lombok Regency

This research aimed to identify the effect of the length of thawing time on the quality  of  Bali  cattle postthaw semen and  to  investigate  the resulting pregnancy  rate. This  research  was  conducted  at  Wanasaba  Village,  specifically  Tanaq  Mira  Village, Wanasaba  District,  East  Lombok  Regency.  Five  samples  of  Bali  cattle  semen  were  collected  for  insemination  from  each  of  three  different  farmer  groups.  The  semen sample  from  the  remaining  frozen  semen  inseminated  by  the  inseminator  was  used  in this research. Tanaq Mira Village's inseminator performed thawing during the trip from the  artificial  insemination  (AI)  station  to  the  farmer  group's  location.  This  research observed  the  microscopic  quality  of  the postthaw frozen  semen  covering  motility, viability, and abnormality. Moreover, the pregnancy rate on AI acceptors using the non return  rate (NRR)  parameter  or  the  number  of  female parents who returned to  estrus after being inseminated were observed. The observation was conducted on three farmer groups  with  different  distances  and  lengths  of  thawing  time.  Each  farmer  group  was observed five times. The collected data were then analysed by using one-way analysis of variance(ANOVA). The research indicated that the distance of the farmer group’s location significantly affected (P0,05) the sperm abnormalities. The Sapeng farmer group had the  highest NRR, as  much  as 80%. According  to the  NRR  score,  it  can  be  concluded that the thawing process  using  the  thermos  within  less  than 10 minutes  resulted  in  the best yield

Supplementation of Follicle Stimulating Hormon Into In vitro Maturation Medium to Increase Oocytes Maturation and 4 Cell Stadium Embryo Development of Bligon Goat

The study was carried out to investigate the effect of follicle stimulating hormon (FSH) into in vitro maturation medium to increase oocytes maturation and 4 cell stadium embryo development of Bligon goat. Goat ovaries were obtained from a slaughterhouse and transported to the laboratory in a flask of NaCl at temperature of 31 – 34°C. Oocytes were aspirated from 2 – 6 mm of follicles into a 3 mL syringe (23G needle) that contained Dulbecco’s Phosphate-Buffered Saline. Oocytes were divided into three groups, i.e tissue culture medium (TCM) with FSH supplementation 0, 50, and 100 IU/mL. Oocytes were put into those medium and incubated on 39°C, 5% CO2, and 95% humidity for 24 hours. Matured oocytes were fertilized with capacitated frozen thawed-semen and incubated on 39°C, 5% CO2, and 95% humidity for 5 hours. Fertilized oocytes were washed for 3 times in TCM and incubated in the same condition for embryo culture. The data of FSH supplementation and embryo development were analyzed using randomized completely one way classification. The results showed that the percentages of mature oocytes from FSH supplementation 0, 50, and 100 IU/mL were 70,48±23,22, 78,48±15,80, and 80,29±12,86%, respectively. Cleavage rate of the two cells stage were 36,00±14,22, 44,00±33,94, and 57,45±31,78%, respectively, and for the 4 cells stage were 27,33±22,04, 35,33±40,73, and 39,45±20,38%. It is concluded that supplementation of FSH in the maturation medium could not increase the percentages of in vitro maturation and embryo development

Single Nucleotide Polymorphism of Partial GDF9 Gene in Three Local Goat of Indonesia Compare with Several Goat in Asia

The GDF9 gene is a gene that affects the maturation of oocytes. GDF9 is expressed in oocytes and granulosa cell, it can stimulate granulose cell proliferation and regulate cumulus cell function from pre-ovulation to ovulation. The GDF9 gene is associated with an increase in the ovulation rate and litter size in animals. This study aims to determine the kinship relationship of local goats compared to goats in Asia on prolific traits and to determine the restriction mapping of the GDF9 gene in goats based on the different SNP locations. The local goat comes from the Bligon goat, Kacang goat and Kejobong goats which is compared to the GenBank data (EF446168, EU883989 and KY780296). GDF9 sequences were analyzed using BioEdit and sequencing results to identify Single Nucleotide Polymorphism (SNP) and using NEBCutter V2 to determine the restriction enzyme which recognized the sequence around SNP. The result shows that three variations of SNP were found in exon 2 (g.3615T>C, g.3760T>C and g.3855A>C). Identification of SNP position found 1 SNP position identified by restriction enzyme at g.3855A> C. The identified restriction enzyme is HpaII and MspI. The results of this study are expected to provide genetic information that will be used for further research on the relationship between GDF9 gene polymorphisms to animal prolific

Effect of Different Pre-Freezing Time on Quality of Frozen Fat-Tailed Ram Semen

This study aimed to determining the effect of temperature changes on the sperm quality of fat-tailed sheep during the freezing process using a microcontroller. This study was conducted from April to October 2018 at the Laboratory of Animal Physiology and Reproduction, Faculty of Animal Science UGM. The study was used twelve mature ram. The methods were conducted by collecting semen using artificial vagina, semen dilution, freezing semen, thawing and semen quality test. The pre-freezing temperature time were grouped for 6, 9, and 12 minutes. Data of frozen semen quality (motility, viability, and abnormality) the data were analyzed using one-way ANOVA and the study was arranged using Completely Randomized Design (CRD). The average of spermatozoa motility after semen freezing with a 5 to -140°C decrease in pre freezing temperature for 12 minutes (50±5.3%) was significantly different (P<0.05) compared to 9 minutes (48±4.8%) and 6 minutes (43±4.8%). The average of spermatozoa viability after semen freezing with a decrease in pre freezing temperature of 5 to -140°C for 12 minutes (55±4.7%) was significantly different (P<0.05) compared to that for 9 minutes (52±3.5%) and 6 minutes (49±5.7%). The average of spermatozoa abnormality after freezing with a decrease in pre freezing temperature of 5 to -140°C for 6, 9, and 12 minutes was not significantly different (10±2.4%, 9±0.8%, and 10±0.9%, respectively). Based on the findings, it is possible to conclude that semen freezing at a lower pre-freezing temperature of 0 to -140°C for 12 minutes can improve the quality of freezing results

Evaluasi Penggunaan Etanol dan Surfaktan Tween 80 dalam Melarutkan Vitamin E pada Bahan Pengencer Sperma Andromed

Penelitian ini dirancang untuk menguji bioavailabilitas vitamin E yang dilarutkan dengan menggunakan pelarut yang berbeda, yaitu surfaktan tween 80 dan etanol pada bahan pengencer semen Andromed. Perlakuan yang diberikan pada penelitian ini adalah bahan antioksidan vitamin E dan bahan pelarut minyak yang terbagi dalam kelompok Andromed kontrol (P0), Andromed  Vit E (0.2 gr) + etanol (P1), Andromed vit.E (0.2 gr) + tween 80 (P2), Andromed vit. E (0.4 gr) + etanol (P3) dan Andromed Vit. E (0.4 gr) + tween 80 (P4). Metode penelitian menggunakan pendekatan eksperimental, dan dianalisis secara deskriptif. Vitamin E dikombinasikan dengan pelarut sesuai perlakuan yang selanjutnya disebut sediaan vitamin E. Sediaan vitamin E kemudian dicampurkan kedalam bahan pengencer.  Sampel bahan pengencer tersebut dianalisis menggunakan analisis DPPH untuk mengukur aktivitas dari vitamin E di dalam bahan pengencer semen. Hasil penelitian menunjukkan nilai aktivitas antioksidan bahan pengencer yang diberikan kombinasi Vit. E dan tween 80 paling tinggi sebesar 91.29% sedangkan bahan pengencer dengan kombinasi Vit. E dan etanol aktivitas antioksidan tertingginya sebesar 57.45% dan hasil paling rendah pada bahan pengencer control tanpa penambahan vitamin E. Tween 80 sebagai pelarut vitamin E memiliki kemampuan yang lebih baik dalam melarutkan vitamin E dalam bahan pengencer semen jika dibandingkan dengan etanol

The Effect of Lycopene Addition on the Semen Quality of Saanen Goats Stored at 5°C for 24 hours

In order to increase the livestock population, a good reproductive process was needed. Lycopene which belongs to carotenoid group has a dark yellow pigment. This study aimed to determine the effect of adding lycopene on the semen quality of Saanen goats. The research design used 4 treatments, P0 (egg yolk tris diluent) as control), P1 (egg yolk tris diluent + 2% lycopene), P2 (egg yolk tris diluent + 4% lycopene), and P3 (yolk tris diluent). egg + 6% lycopene). The quality of semen was observed after 24 hours of storage at 5℃. Macroscopic data were analyzed descreptively and the microscopic one was analyzed using a completely randomized design (CRD). The results showed that the addition of lycopene in egg yolk tris diluent had a significant (P<0.05) effect on the semen quality of Saanen goats. The results showed that the motility of spermatozoa in each treatment group (P0, P1, P2, and P3) was 60.1 ± 5.7%, 65.2 ± 4.3%, 62.4 ± 5.2%, and 60.6 ± 4.7%, respectively, viability were 65.3 ± 4.8, 73.5 ± 4.2, 70.4 ± 3.8, and 66.1 ± 5.1, respectively, abnormality were 17 ± 1.8%, 16 ± 1.8%, 18 ± 1.9%, and 16 ± 1.7%, respectively, and membrane integrity were 63 ± 3.3%, 70 ± 3.6 %, 67 ± 3.2%, and 62 ± 2.9%, respectively. Based on the results, it could be concluded that the lycopene addition improved the semen quality of Saanen goats compared to controls