A high performance microfluidic analyser for phosphate measurements in marine waters using the vanadomolybdate method

We report a high performance autonomous analytical system based on the vanadomolybdate method for the determination of soluble reactive phosphorus in seawater. The system combines a microfluidic chip manufactured from tinted poly (methyl methacrylate) (PMMA), a custom made syringe pump, embedded control electronics and on-board calibration standards. This “lab-on-a-chip” analytical system was successfully deployed and cross-compared with reference analytical methods in coastal (south west England) and open ocean waters (tropical North Atlantic). The results of the miniaturized system compared well with a reference bench-operated phosphate auto-analyser and showed no significant differences in the analytical results (student’s t-test at 95% confidence level). The optical technology used, comprising of tinted PMMA and polished fluidic channels, has allowed an improvement of two orders of magnitude of the limit of detection (52 nM) compared to currently available portable systems based on this method. The system has a wide linear dynamic range 0.1–60 μM, and a good precision (13.6% at 0.4 μM, n=4). The analytical results were corrected for silicate interferences at 0.7 μM, and the measurement frequency was configurable with a sampling throughput of up to 20 samples per hour. This portable micro-analytical system has a low reagent requirement (340 μL per sample) and power consumption (756 J per sample), and has allowed accurate high resolution measurements of soluble reactive phosphorus in seawater

Collective phenomena in crowds—Where pedestrian dynamics need social psychology

This article is on collective phenomena in pedestrian dynamics during the assembling and dispersal of gatherings. To date pedestrian dynamics have been primarily studied in the natural and engineering sciences. Pedestrians are analyzed and modeled as driven particles revealing self-organizing phenomena and complex transport characteristics. However, pedestrians in crowds also behave as living beings according to stimulus-response mechanisms or act as human subjects on the basis of social norms, social identities or strategies. To show where pedestrian dynamics need social psychology in addition to the natural sciences we propose the application of three categories–phenomena, behavior and action. They permit a clear discrimination between situations in which minimal models from the natural sciences are appropriate and those in which sociological and psychological concepts are needed. To demonstrate the necessity of this framework, an experiment in which a large group of people (n = 270) enters a concert hall through two different spatial barrier structures is analyzed. These two structures correspond to everyday situations such as boarding trains and access to immigration desks. Methods from the natural and social sciences are applied. Firstly, physical measurements show the influence of the spatial structure on the dynamics of the entrance procedure. Density, waiting time and speed of progress show large variations. Secondly, a questionnaire study (n = 60) reveals how people perceive and evaluate these entrance situations. Markedly different expectations, social norms and strategies are associated with the two spatial structures. The results from the questionnaire study do not always conform to objective physical measures, indicating the limitations of models which are based on objective physical measures alone and which neglect subjective perspectives