Mechanisms underlying heterologous skin scaffold-mediated tissue remodeling

Biocompatibility of two newly developed porcine skin scaffolds was assessed after 3, 14, 21 and 90 days of implantation in rats. Both scaffolds showed absence of cells, preservation of ECM and mechanical properties comparable to non-decellularised skin before implantation. Host cell infiltration was much prominent on both scaffolds when compared to Permacol (surgical control). At day 3, the grafts were surrounded by polymorphonuclear cells, which were replaced by a notable number of IL-6-positive cells at day 14. Simultaneously, the number of pro-inflammatory M1-macrophage was enhanced. Interestingly, a predominant pro-remodeling M2 response, with newly formed vessels, myofibroblasts activation and a shift on the type of collagen expression was sequentially delayed (around 21 days). The gene expression of some trophic factors involved in tissue remodeling was congruent with the cellular events. Our findings suggested that the responsiveness of macrophages after non-crosslinked skin scaffolds implantation seemed to intimately affect various cell responses and molecular eventsand this range of mutually reinforcing actions was predictive of a positive tissue remodeling that was essential for the long-standing success of the implants. Furthermore, our study indicates that non-crosslinked biologic scaffold implantation is biocompatible to the host tissue and somehow underlying molecular events involved in tissue repair.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fed Univ Sao Paulo UNIFESP, Postgrad Struct & Funct Biol, BR-04023900 Sao Paulo, SP, BrazilSao Paulo State Univ UNESP, Dept Biol, BR-15054000 Sao Jose Do Rio Preto, SP, BrazilSao Paulo State Univ UNESP, Inst Biociencias Letras & Ciencias Exatas, BR-15054000 Sao Jose Do Rio Preto, SP, BrazilUCL, Northwick Pk Inst Med Res, Dept Surg Res, London HA1 3UJ, Middx, EnglandPost-Graduation in Structural and Functional Biology, Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, 04023-900, BrazilFAPESP: 2012/21603-2FAPESP: 2012/13041-4FAPESP: 2014/18557-4CNPq: 308144/2014-7CNPq: 245859/2012-8Web of Scienc

Arabic within culture forensic interviews : Arabic native speaking lay-observer truth and lie accuracy, confidence, and verbal cue selection

Cross cultural differences in behavioral and verbal norms and expectations can undermine credibility, often triggering a lie bias which can result in false convictions. However, current understanding is heavily North American and Western European centric, hence how individuals from non-western cultures infer veracity is not well understood. We report novel research investigating native Arabic speakers’ truth and lie judgments having observed a matched native language forensic interview with a mock person of interest. 217 observers viewed a truthful or a deceptive interview and were directed to attend to detailedness as a veracity cue or given no direction. Overall, a truth bias (66% accuracy) emerged, but observers were more accurate (79%) in the truth condition with the truthful interviewee rated as more plausible and more believable than the deceptive interviewee. However, observer accuracy dropped to just 23% when instructed to use the detailedness cue when judging veracity. Verbal veracity cues attended too were constant across veracity conditions with ‘corrections’ emerging as an important veracity cue. Some results deviate from the findings of research with English speaking western participants in cross- and matched-culture forensic interview contexts, but others are constant. Nonetheless, this research raises questions for research to practice in forensic contexts centred on the robustness of western centric psychological understanding for non-western within culture interviews centred on interview protocols for amplifying veracity cues and the instruction to note detailedness of verbal accounts which significantly hindered Arabic speaker’s performance. Findings again highlight the challenges of pancultural assumptions for real-world practices

Quantitative analysis of the development of experimentally induced post surgical adhesions: a microstereological study

The aim of this study was to quantitatively define the development of post surgical adhesions (PSAs) in a well characterized experimental model and identify possible windows of pathogenesis where pharmaceutical intervention may be most effective