Swertia chirata- A Wonderful Herb

The Binomial Name is Swertia chirata Buch Ham. Because it grows in Nepal’s forests, S.chirata is also known as Nepali Neem. Since the 18th century, India has used S.chirata as a medicinal plant, which Europe has introduced and recommended abroad. It can potentially treat anti-inflammatory, hepatoprotective, anti-bacterial, wound healing, hypoglycaemic, antioxidant, antitussive, antipyretic, anti-diabetic, anti-cancer, anti-tumor. It belongs to the Family: Gentianaceae, Genus: Swertia, and Species: Chirata. This review article contains all the relevant information regarding its morphology, microscopy, phytochemistry, plant description, taxonomy, substitutes of S. chirata, pharmacological activities of the plant. The review article aims to provide brief knowledge about this drug among people and researchers

Potential Candidate Molecules of Past and Present for Combating High Altitude Hypoxia Induced Maladies

Hypobaric hypoxia occurs at high altitudes where barometric pressure is low causing insufficient supply of oxygen leading to many high-altitude illnesses like acute mountain sickness (AMS), high altitude pulmonary edema (HAPE), high altitude cerebral edema (HACE) etc.Medications have been applied to treat and prevent injuries caused by HBH, showing anti-inflammatory, anti-edemagenic, and antioxidant properties. AMS symptoms, such as headache, nausea, weariness, usually go away in 1-2 days. HACE causes brain swelling, elevated intracranial pressure, resulting in confusion, stupor, ataxia, and death.Acetazolamide, dexamethasone, nifedipine are the drugs used for treatment acting oncarbonic anhydrase enzyme, calcium channels.Acetazolamide increases arterial partial pressure of oxygen.Nifedipine relaxes vascular smooth muscles and increases blood flow. Some drugscause side effects also like dizziness, diuresis, nausea, malaise, etc. Hence, a new drug search is needed to find more targeted and fewer side effects for faster relief and better health at high altitudes

Tending the Taproot: Opportunities to Support Folk & Traditional Arts in the United States

This report presents the findings of the Alliance of California Traditional Arts' (ACTA) Taproot Initiative. This national planning effort, aimed to re-center traditional and folk artists and their art forms as catalysts for transformation and restoration in our larger society, is aligned with other important movements in the arts and culture sector to spur critical thinking and action during this hallmark moment of radical change. The report describes the resource landscape of folk and traditional arts. It offers operational recommendations as a call to action to support taproot artist-leaders and organizations with focused investment in funding and development to do more and do better, resources for infrastructure, elevated national recognition, and new standards for robust data and research infrastructure. Our recommendations are evidenced by qualitative and quantitative research findings, grounded in ACTA's quarter century of experience as a funder and advocate in this field

Yeast expressed recombinant Hemagglutinin protein of Novel H1N1 elicits neutralising antibodies in rabbits and mice

Currently available vaccines for the pandemic Influenza A (H1N1) 2009 produced in chicken eggs have serious impediments viz limited availability, risk of allergic reactions and the possible selection of sub-populations differing from the naturally occurring virus, whereas the cell culture derived vaccines are time consuming and may not meet the demands of rapid global vaccination required to combat the present/future pandemic. Hemagglutinin (HA) based subunit vaccine for H1N1 requires the HA protein in glycosylated form, which is impossible with the commonly used bacterial expression platform. Additionally, bacterial derived protein requires extensive purification and refolding steps for vaccine applications. For these reasons an alternative heterologous system for rapid, easy and economical production of Hemagglutinin protein in its glycosylated form is required. The HA gene of novel H1N1 A/California/04/2009 was engineered for expression in Pichia pastoris as a soluble secreted protein. The full length HA- synthetic gene having α-secretory tag was integrated into P. pastoris genome through homologous recombination. The resultant Pichia clones having multiple copy integrants of the transgene expressed full length HA protein in the culture supernatant. The Recombinant yeast derived H1N1 HA protein elicited neutralising antibodies both in mice and rabbits. The sera from immunised animals also exhibited Hemagglutination Inhibition (HI) activity. Considering the safety, reliability and also economic potential of Pichia expression platform, our preliminary data indicates the feasibility of using this system as an alternative for large-scale production of recombinant influenza HA protein in the face of influenza pandemic threat

Constructing Diasporic Identity Through Kathak Dance: Flexibility, Fixity, and Nationality in London and Los Angeles

This dissertation focuses on the role of the classical Indian dance form Kathak in negotiating questions of cultural identity and national affiliation among members of the Indian diaspora residing in London, UK, and Los Angeles, USA. This study considers how institutional actions and discourses related to the practice of Kathak dance in these two cities and the personal experiences of dancers themselves reflect certain political, aesthetic, and social values that impact the formation of diasporic identity. Grounded in multi-year fieldwork in the Kathak communities of London and Los Angeles, this interdisciplinary study argues that Indian diasporic subjects negotiate a fundamental tension through their practice of Kathak dance: the tension between Kathak’s inherent flexibility and contextual conditions of fixity.Kathak’s inherent flexibility refers to certain foundational elements of the dance that center around creative interpretation, improvisation, and immersive practice (riyaaz), as well as the expression of multiple identities that these foundational elements enable. A discourse of Kathak’s flexibility frames the dancer’s transcendence and/or transformation of socially assigned identifications as an act of aesthetic virtuosity with cross-cultural significance. However, the transformative potential of Kathak’s flexibility is limited by contextual conditions of fixity that include material and discursive frameworks that fix Kathak to regimes of affect and singular narratives that essentialize the dance as a marker of Indian national identity and curtail the breadth of flexible identifications for both the dance and its dancers. This dissertation explores two dominant and interconnected strategies that shape Kathak discourse and practice towards different socio-political ends. The heritage model practice of Kathak aims to empower Indian diasporic subjects by underscoring their difference from the dominant British or American classes. Drawing from Annette Weiner’s work on inalienable possessions, this study offers a new theory that casts Kathak in the heritage model as an inalienable practice ontologically tied to a singularized Indian cultural identity. Conversely, the integrative model frames Kathak as a tool for intercultural encounters, utilizing the dance’s inherent flexibility to create new pathways for Kathak dance and dancers to enter and/or converse with British and American national culture

Hepatitis E Virus Cysteine Protease Has Papain Like Properties Validated by in silico Modeling and Cell-Free Inhibition Assays

Hepatitis E virus (HEV) has emerged as a global health concern during the last decade. In spite of a high mortality rate in pregnant women with fulminant hepatitis, no antiviral drugs or licensed vaccine is available in India. HEV-protease is a pivotal enzyme responsible for ORF1 polyprotein processing leading to cleavage of the non-structural enzymes involved in virus replication. HEV-protease region encoding 432–592 amino acids of Genotype-1 was amplified, expressed in Sf21 cells and purified in its native form. The recombinant enzyme was biochemically characterized using SDS-PAGE, Western blotting and Immunofluorescence. The enzyme activity and the inhibition studies were conducted using Zymography, FTC-casein based protease assay and ORF1 polyprotein digestion. To conduct ORF1 digestion assay, the polyprotein, natural substrate of HEV-protease, was expressed in E. coli and purified. Cleavage of 186 kDa ORF1 polyprotein by the recombinant HEV-protease lead to appearance of non-structural proteins viz. Methyltransferase, Protease, Helicase and RNA dependent RNA polymerase which were confirmed through immunoblotting using antibodies generated against specific epitopes of the enzymes. FTC-casein substrate was used for kinetic studies to determine Km and Vmax of the enzyme and also the effect of different metal ions and other protease inhibitors. A 95% inhibition was observed with E-64 which was validated through in silico analysis. The correlation coefficient between inhibition and docking score of Inhibitors was found to have a significant value of r2 = 0.75. The predicted 3D model showed two domain architecture structures similar to Papain like cysteine protease though they differed in arrangements of alpha helices and beta sheets. Hence, we propose that HEV-protease has characteristics of “Papain-like cysteine protease,” as determined through structural homology, active site residues and class-specific inhibition. However, conclusive nature of the enzyme remains to be established

Impact of brass and electroplating industry effluent on some physicochemical and biological properties of soil

957-962A field study was conducted at three sites (S1-S3) of 500m longitudinal transect along the drain, where soil was irrigated by toxic metal rich brass and electroplating industrial effluent in suburban area of Moradabad, India. Spatial and seasonal variations of total heavy metal content in soil and their effect on physico-chemical and biological properties were studied in soil samples taken from 0-25, 25-50 and 50-75 cm soil depth. Except pH, all parameters were maximum in summer in surface soil at each site. Total metal content decreased with increasing distance from the effluent course. Physico-chemical values (pH, EC, Corg, available N etc.) were found minimum at S1 and maximum at S3. Significant inhibition of microbial biomass C and N, respiration, dehydrogenase activity and microbial coefficient occurred in soils highly contaminated by heavy metals. There was a significant decrease in Cmic/Nmic ratio and an increase in metabolic quotient (qCO₂) with increasing metal concentration

Intricacies of using temperature of different niches for assessing impact on malaria transmission

Background & objectives: The influence of temperature on the life cycle of mosquitoes as well as on development of malaria parasite in mosquitoes is well studied. Most of the studies use outdoor temperature for understanding the transmission dynamics and providing projections of malaria. As the mosquitoes breed in water and rest usually indoors, it is logical to relate the transmission dynamics with temperature of micro-niche. The present study was, therefore, undertaken to understand the influence of different formats of temperature of different micro-niches on transmission of malaria for providing more realistic projections. Methods: The study was conducted in one village each of Assam and Uttarakhand s0 tates of India. Temperatures recorded from outdoor (air) as well as indoor habitats (resting place of mosquito) were averaged into daily, fortnightly and monthly and were used for determination of transmission windows (TWs) for Plasmodium vivax (Pv) and P. falciparum (Pf) based on minimum temperature threshold required for transmission. Results: The daily temperature was found more useful for calculation of sporogony than fortnightly and monthly temperatures. Monthly TWs were further refined using fortnightly temperature, keeping in view the completion of more than one life cycle of malaria vectors and sporogony of malaria parasite in a month. A linear regression equation was generated to find out the relationship between outdoor and indoor temperatures and R [2] to predict the percentage of variation in indoor temperature as a function of outdoor temperature at both localities. Interpretation & conclusions: The study revealed that the indoor temperature was more than outdoors in stable malarious area (Assam) but fluctuating in low endemic area like Uttarakhand. Transmission windows of malaria should be determined by transforming outdoor data to indoor and preferably at fortnightly interval. With daily recorded temperature, sporogonic and gonotrophic cycles can also be calculated which is otherwise not possible with monthly data. The study highlights that the projections made for malaria in view of climate change need to be seen with limitation of difference in outdoor and indoor temperatures at different locations, highlighting the need for local data generation at least at sub-district level

Preliminary results on a-SiC:H based thin film light emitting diode by hot wire CVD

Preliminary results on the first hot wire deposited a-SiC:H based thin film light emitting p–i–n diode having the structure glass/TCO(SnO2:F)/p-a-SiC:H/i-SiC:H/n-a-SiC:H/Al are reported. The paper discusses the results of our attempts to optimize the p-, i- and the n-layers for the desired electrical and optical properties. The optimized p-layers have a bandgap Eg∼2 eV and conductivity a little lower than 10−5 (Ω cm)−1. On the other hand, the optimized n-type a-SiC:H show a conductivity of ∼10−4 (Ω cm)−1 with bandgap 2.06 eV. The highest bandgap of the intrinsic layer is approximately 3.4 eV and shows room temperature photoluminescence peak at approximately 2.21 eV. Thin film p–i–n diodes having i-layers with Eg from 2.7 to 3.4 eV show white light emission at room temperature under forward bias of >5 V. However, the 50-nm thick devices show appreciable reverse leakage current and a low emission intensity, which we attribute to the contamination across the p–i interface since these devices are made in a single chamber with the same filament