The new face of alternative assessment in accounting sciences - technology as an anthropomorphic stakeholder

Worldwide, more and more higher education institutions are making use of online or distance education, due to the increasing demand for tertiary education. In this article, the problem addressed relates to the importance of technology as a stakeholder in alternative assessments for undergraduate accounting sciences modules in an online, non-venue-based, technology-enhanced environment. The purpose of this article is to demonstrate how technology, due to its importance and impact, acts similarly as a human stakeholder and should be treated as an anthropomorphic stakeholder in an online technology-enhanced environment, considering its powerful role. A qualitative approach was followed, and action research was used to develop a conceptual framework. This conceptual framework demonstrates how stakeholder theory could ensure the legitimacy of qualifications through identity verification of students in an online environment through improved performance and innovation potential. These findings contribute to the body of knowledge and due to its importance, technology could be considered as an anthropomorphic stakeholder in the creation, improvement, or restructuring of assessments at online educational institutions

Catching on to concatenation : evidence for prepollination intrasexual selection in plants

[from introduction] Darwin (1871) proposed the mechanism of sexual selection to explain the extreme traits, secondary sex characters, seen primarily in male animals for either display or competition. These elaborate traits for attracting members of the opposite sex or competing for access to mates, inter- and intra-sexual selection, respectively, would increase the mating success of individuals but may reduce individual survival. Sexual selection has traditionally been associated with motile, dioecious animals that have well-developed sensory abilities and has long been viewed as absent as a mode of selection in plants (Grant, 1995). However, since the 1980s, several workers have argued that sexual selection is applicable to plants and should be considered as a factor in floral evolution (Skogsmyr & Lankinen, 2002). Opponents such as Grant (1995) point to incompatibility with Darwin’s usage, hermaphroditism, the absence of obvious secondary sex characters and the apparent absence of female choice in plants (Skogsmyr & Lankinen, 2002; Moore & Pannell, 2011). Proponents, however, argue that historical definitions should be expanded to explain modern evidence and that sexual selection can, in principle, occur in hermaphrodites and hence play a role in the evolution of floral traits. The latter view is supported by a recent study by Cocucci et al. (this issue of New Phytologist, pp. 280–286) which demonstrates intra-sexual selection in plants via direct male–male competition between the pollinaria of milkweeds for optimal attachment sites on pollinators to ensure subsequent pollinium deposition. This is the first example of male–male competition resulting in secondary sex characters in hermaphrodite plants, highlighting the possibility of overlooked mechanisms of sexual selection in plants

Vascular endothelial growth factor can signal through platelet-derived growth factor receptors

Vascular endothelial growth factor (VEGF-A) is a crucial stimulator of vascular cell migration and proliferation. Using bone marrow–derived human adult mesenchymal stem cells (MSCs) that did not express VEGF receptors, we provide evidence that VEGF-A can stimulate platelet-derived growth factor receptors (PDGFRs), thereby regulating MSC migration and proliferation. VEGF-A binds to both PDGFRα and PDGFRβ and induces tyrosine phosphorylation that, when inhibited, results in attenuation of VEGF-A–induced MSC migration and proliferation. This mechanism was also shown to mediate human dermal fibroblast (HDF) migration. VEGF-A/PDGFR signaling has the potential to regulate vascular cell recruitment and proliferation during tissue regeneration and disease

Recording, analysis, and interpretation of spreading depolarizations in neurointensive care: Review and recommendations of the COSBID research group.

Spreading depolarizations (SD) are waves of abrupt, near-complete breakdown of neuronal transmembrane ion gradients, are the largest possible pathophysiologic disruption of viable cerebral gray matter, and are a crucial mechanism of lesion development. Spreading depolarizations are increasingly recorded during multimodal neuromonitoring in neurocritical care as a causal biomarker providing a diagnostic summary measure of metabolic failure and excitotoxic injury. Focal ischemia causes spreading depolarization within minutes. Further spreading depolarizations arise for hours to days due to energy supply-demand mismatch in viable tissue. Spreading depolarizations exacerbate neuronal injury through prolonged ionic breakdown and spreading depolarization-related hypoperfusion (spreading ischemia). Local duration of the depolarization indicates local tissue energy status and risk of injury. Regional electrocorticographic monitoring affords even remote detection of injury because spreading depolarizations propagate widely from ischemic or metabolically stressed zones; characteristic patterns, including temporal clusters of spreading depolarizations and persistent depression of spontaneous cortical activity, can be recognized and quantified. Here, we describe the experimental basis for interpreting these patterns and illustrate their translation to human disease. We further provide consensus recommendations for electrocorticographic methods to record, classify, and score spreading depolarizations and associated spreading depressions. These methods offer distinct advantages over other neuromonitoring modalities and allow for future refinement through less invasive and more automated approaches

The role of the t-SNARE SNAP-25 in action potential-dependent calcium signaling and expression in GABAergic and glutamatergic neurons

Abstract Background The soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex, comprised of SNAP-25, syntaxin 1A, and VAMP-2, has been shown to be responsible for action potential (AP)-dependent, calcium-triggered release of several neurotransmitters. However, this basic fusogenic protein complex may be further specialized to suit the requirements for different neurotransmitter systems, as exemplified by neurons and neuroendocrine cells. In this study, we investigate the effects of SNAP-25 ablation on spontaneous neuronal activity and the expression of functionally distinct isoforms of this t-SNARE in GABAergic and glutamatergic neurons of the adult brain. Results We found that neurons cultured from Snap25 homozygous null mutant (Snap25-/-) mice failed to develop synchronous network activity seen as spontaneous AP-dependent calcium oscillations and were unable to trigger glial transients following depolarization. Voltage-gated calcium channel (VGCC) mediated calcium transients evoked by depolarization, nevertheless, did not differ between soma of SNAP-25 deficient and control neurons. Furthermore, we observed that although the expression of SNAP-25 RNA transcripts varied among neuronal populations in adult brain, the relative ratio of the transcripts encoding alternatively spliced SNAP-25 variant isoforms was not different in GABAergic and glutamatergic neurons. Conclusion We propose that the SNAP-25b isoform is predominantly expressed by both mature glutamatergic and GABAergic neurons and serves as a fundamental component of SNARE complex used for fast synaptic communication in excitatory and inhibitory circuits required for brain function. Moreover, SNAP-25 is required for neurons to establish AP-evoked synchronous network activity, as measured by calcium transients, whereas the loss of this t-SNARE does not affect voltage-dependent calcium entry.</p

Disease, invasions and conservation : no evidence of squirrelpox virus in grey squirrels introduced to Italy

Native red squirrels Sciurus vulgaris in Great Britain and Ireland are threatened by alien grey squirrels S. carolinensis through exploitation competition and spillover of squirrelpox virus (SQPV). By accelerating the replacement of red squirrels by the invader, SQPV represents a fundamental factor to consider when planning management and conservation strategies. In mainland Europe, grey squirrels introduced to Italy threaten the survival of the whole continental red squirrel population, but no extensive surveys for SQPV presence have been carried out in the region. We therefore investigated SQPV infection in north Italian grey squirrel populations through a combination of serological and molecular methods. Firstly, we analysed sera from 285 individuals through an enzyme\u2010linked immunosorbent assay (ELISA) to detect antibodies against SQPV. Secondly, a PCR designed to amplify a segment of the G8R SQPV gene was carried out on DNA extracted from swabs and skin tissue samples from a second set of 66 grey squirrels. ELISA tests identified four reactors (1.4%), but the subsequent PCR survey did not detect any SQPV DNA. Based on the low prevalence observed and on PCR results, we believe that the four suspected positives were the result of an ELISA cross\u2010reaction following exposure to another pox virus. Considering sample size and performances of the two methods, confidence of freedom from SQPV resulted above 99.9%. However, because of the severe impact of SQPV on red squirrels, we recommend the implementation of a passive surveillance plan for the early detection of an SQPV emergence in continental Europe

The continuum of spreading depolarizations in acute cortical lesion development: Examining Leao's legacy.

A modern understanding of how cerebral cortical lesions develop after acute brain injury is based on Aristides Leao's historic discoveries of spreading depression and asphyxial/anoxic depolarization. Treated as separate entities for decades, we now appreciate that these events define a continuum of spreading mass depolarizations, a concept that is central to understanding their pathologic effects. Within minutes of acute severe ischemia, the onset of persistent depolarization triggers the breakdown of ion homeostasis and development of cytotoxic edema. These persistent changes are diagnosed as diffusion restriction in magnetic resonance imaging and define the ischemic core. In delayed lesion growth, transient spreading depolarizations arise spontaneously in the ischemic penumbra and induce further persistent depolarization and excitotoxic damage, progressively expanding the ischemic core. The causal role of these waves in lesion development has been proven by real-time monitoring of electrophysiology, blood flow, and cytotoxic edema. The spreading depolarization continuum further applies to other models of acute cortical lesions, suggesting that it is a universal principle of cortical lesion development. These pathophysiologic concepts establish a working hypothesis for translation to human disease, where complex patterns of depolarizations are observed in acute brain injury and appear to mediate and signal ongoing secondary damage

Research Note:<br>Derivation of temperature lapse rates in semi-arid south-eastern Arizona

International audienceEcological and hydrological modelling at the regional scale requires distributed information on weather variables, and temperature is important among these. In an area of basin and range topography with a wide range of elevations, such as south-eastern Arizona, measurements are usually available only at a relatively small number of locations and elevations, and temperatures elsewhere must be estimated from atmospheric lapse rate. This paper derives the lapse rates to estimate maximum, minimum and mean daily temperatures from elevation. Lapse rates were calculated using air temperatures at 2 m collected during 2002 at 18 locations across south-eastern Arizona, with elevations from 779 to 2512 m. The lapse rate predicted for the minimum temperature was lower than the mean environmental lapse rate (MELR), i.e. 6 K km?1, whereas those predicted for the mean and maximum daily temperature were very similar to the MELR. Lapse rates were also derived from radiosonde data at 00 and 12 UTC (5 pm and 5 am local time, respectively). The lapse rates calculated from radiosonde data were greater than those from the 2 m measurements, presumably because the effect of the surface was less. Given temperatures measured at Tucson airport, temperatures at the other sites were predicted using the different estimates of lapse rates. The best predictions of temperatures used the locally predicted lapse rates. In the case of maximum and mean temperature, using the MELR also resulted in accurate predictions. Keywords: near surface lapse rates, semi-arid climate, mean minimum and maximum temperatures, basin and range topograph

Fibrillin-Rich Microfibrils are Reduced in Photoaged Skin. Distribution at the Dermal–Epidermal Junction

Chronic sun exposure results in photoaged skin with deep coarse wrinkles and loss of elasticity. We have examined the distribution and abundance of fibrillin-rich microfibrils, key structural components of the elastic fiber network, in photoaged and photoprotected skin. Punch biopsies taken from photoaged forearm and from photoprotected hip and upper inner arm of 16 subjects with a clinical range of photoaging were examined for fibrillin-1 and fibrillin-2 expression and microfibril distribution. In situ hybridization revealed decreased fibrillin-1 mRNA but unchanged fibrillin-2 mRNA levels in severely photoaged forearm biopsies relative to photoprotected dermal sites. An immunohistochemical approach demonstrated that microfibrils at the dermal–epidermal junction were significantly reduced in moderate to severely photoaged forearm skin. Confocal microscopy revealed that the papillary dermal microfibrillar network was truncated and depleted in photoaged skin. These studies highlight that the fibrillin-rich microfibrillar network associated with the upper dermis undergoes extensive remodeling following solar irradiation. These changes may contribute to the clinical features of photoaging, such as wrinkle formation and loss of elasticity

Factors that motivate millennial accountancy professionals in industry to become academics

Orientation: Exploring the reasons why millennial chartered accountants choose to pursue a career in academia is fundamental to the future and advancement of the accountancy profession. Research purpose: This article examines the factors that could motivate a millennial accountancy professional to join academia and to describe the lived experiences of accountancy academics in the context of career identity and situational circumstances from London’s theory of career motivation. Motivation for the study: Tertiary accountancy education holds a national interest, which elevates the importance of attracting and retaining suitably qualified accountancy lecturers. Research approach/design and method: As part of interpretative phenomenological analysis, semi-structured face-to-face interviews were conducted with 14 current and former open distance learning accountancy academics in South Africa. Main findings: The research showed that flexible work schedules, the opportunity to teach, work in an autonomous environment and the desire to improve society were significant drivers for professional accountants to join academia. Research ranked as an insignificant motivating factor to join academia, although their retrospective views show the importance for those hoping to remain in academia to embrace it. Practical/managerial implications: This study may be useful to current and aspiring millennial accountancy academics, at universities offering accountancy or chartered accountancy programmes and to their human resource practitioners. Contribution/value-add: The research clarified the prospective perceptions and multi-layered motivational factors that could influence millennial accountancy professionals to join academia as well as the retrospective views from the lived experiences of accountancy lecturers in the context of London’s theory of career motivation