7 research outputs found
Prediction of phosphate concentrate grade based on artificial neural network modeling
In order to determine the grade of phosphate concentrate rapidly and accurately on-line, first, the surface color parameters of primary ore, concentrated and tailing of phosphate rock was collected and extracted based on a self-designing surface color acquisition device. Then these parameters were modeled by artificial neural network. The results showed that the 5-12-1 BP model established by artificial neural network could achieve better prediction results with error less than 5%, which provided theoretical support for realizing the on-line soft measurement of flotation concentrate grade. Keywords: Artificial neural network, Phosphate concentrate, Grade prediction, Color senso
Potassium Transporter LrKUP8 Is Essential for K+ Preservation in Lycium ruthenicum, A Salt-Resistant Desert Shrub
Human iPSC-derived microglia assume a primary microglia-like state after transplantation into the neonatal mouse brain.
Microglia are essential for maintenance of normal brain function, with dysregulation contributing to numerous neurological diseases. Protocols have been developed to derive microglia-like cells from human induced pluripotent stem cells (hiPSCs). However, primary microglia display major differences in morphology and gene expression when grown in culture, including down-regulation of signature microglial genes. Thus, in vitro differentiated microglia may not accurately represent resting primary microglia. To address this issue, we transplanted microglial precursors derived in vitro from hiPSCs into neonatal mouse brains and found that the cells acquired characteristic microglial morphology and gene expression signatures that closely resembled primary human microglia. Single-cell RNA-sequencing analysis of transplanted microglia showed similar cellular heterogeneity as primary human cells. Thus, hiPSCs-derived microglia transplanted into the neonatal mouse brain assume a phenotype and gene expression signature resembling that of resting microglia residing in the human brain, making chimeras a superior tool to study microglia in human disease
An ultrasensitive ratiometric electrochemiluminescence immunosensor combining photothermal amplification for ovarian cancer marker detection
Microcystin-LR Promotes Melanoma Cell Invasion and Enhances Matrix Metalloproteinase-2/‑9 Expression Mediated by NF-κB Activation
This study aimed to explore the molecular mechanisms
behind the
stimulation effects of microcystin-LR (a well-known cyanobacterial
toxin produced in eutrophic lakes or reservoirs) on cancer cell invasion
and matrix metalloproteinases (MMPs) expression. Boyden chamber assay
showed that microcystin-LR exposure (>12.5 nM) evidently enhanced
the invasion ability of the melanoma cells (MDA-MB-435). Tumor Metastasis
PCR Array demonstrated that 24 h microcystin-LR treatment (25 nM)
caused overexpression of eight genes involved in tumor metastasis,
including MMP-2, MMP-9, and MMP-13. Quantitative real-time PCR, Western
blotting and gelatin zymography consistently demonstrated that mRNA
and protein levels of MMP-2/-9 were increased in the cells after microcystin-LR
exposure (<i>P</i> < 0.05 each). Immunofluorescence assay
and electrophoretic mobility shift assay revealed that microcystin-LR
could activate nuclear factor kappaB (NF-κB) by accelerating
NF-κB translocation into the nucleus and enhancing NF-κB
binding ability. Furthermore, addition of NF-κB inhibitor in
culture medium could suppress the invasiveness enhancement and MMP-2/-9
overexpression. This study indicates that microcystin-LR can act as
a NF-κB activator to promote MMP-2/-9 expression and melanoma
cell invasion, which deserves more environmental health concerns