Advanced drilling detection and multi-information identification of water-conducting channel of coal floor

The coal floor develops various water-conducting channels, which seriously threaten the safe production of mines. In order to establish a more scientific multi-information identification technology system for the water-conducting channels in the coal floor, and prevent floor water inrush. Firstly, the principles of reasonable trajectory, reasonable target layer and exploration area maximization are proposed based on the characteristics of the water-conducting channels and the advantages of the ground directional drilling area exploration technology. Diversified types, unclear locations, and significant water inrush hazards are the characteristics of water-conducting channels. Secondly, the summary analysis is conducted on the exploration process of water diversion channels in areas such as HuaiBei Mining Area, Huainan Mining Area, Xingtai Mining Area, and Huanghebei Coal Field. When revealing the water-conducting channels, there are significant differences in visual indicators such as rock debris, drilling time, drilling fluid leakage, and confirmatory indicators such as permeability and grouting parameters compared to revealing normal formations. Therefore, the identification indicators of multi-information during the drilling process are divided into two types: qualitative and quantitative. Based on the variation amplitude of two qualitative indicators, rock debris and drilling time, when encountering water-conducting channels, it gives corresponding standard curves, and determines the classification system for water-conducting channel types. Subsequently, a comprehensive analysis is conducted on the changes in the two quantitative indicators of drilling fluid leakage and permeability when encountering water-conducting channels. 30 m3/h of drilling fluid leakage and 10 Lu of permeability are proposed as the classification criteria for conductivity. Based on this, a dual factor comprehensive classification system for water-conducting channels conductivity is established, and the conductivity is divided into four levels. Finally, taking a typical mine in the North China coalfield as a case study, the principle of exploration is adopted to explore the water-conducting channels. The multi-information identification technology is applied to successfully identify four faults, two karst fracture zones and a collapse in the detection area, and determines conductivity levels of water-conducting channels. The research results have guiding effect and important significance for improving the identification and control of water-conducting channels

Characteristics and prevention and control techniques of typical water hazards in coal mines in Shaanxi Province

Shaanxi province is rich in coal resources and is one of the main coal-producing provinces in China, with a coal production of 746 million tons in 2022. Due to the obvious differences in geological and hydrogeological conditions in the coal mining areas of the province, there are various types of water disasters in its coal mines. With the increase of coal mining intensity in recent years, the frequency of water disasters increases significantly, and the situation of water prevention and control is challenging. On the basis of the overall analysis of the hydrogeological conditions in the coal mining areas of Shaanxi province, combined with the types of water disasters revealed in the mining process of various mining areas in the province, the regional distribution of all kinds of water disas-ters is systematically summarized, and the formation mechanism and characteristics of typical water disasters are discussed. Also, the corresponding prevention and control technology and progress are put forward. The results show that ① the main coal producing areas in Shaanxi province are divided into northern Shaanxi, Huanglong and Weibei. The Jurassic coalfield in northern Shaanxi is mainly affected by the water disaster of roof's loose sand layer, the water disaster of thick sandstone, water and sand burst disaster, and the burnt rock flood exists in some coal mines. The Huanglong coalfield is mainly threatened by roof super-thick sandstone water, separated layer water, mud and sand burst disaster or hidden danger. The Carboniferous-Permian coalfields in northern Shaanxi and Weibei are mainly threatened by the water damage of Ordovician limestone on the coal seam floor. ② The hidden danger of roof flood in the Jurassic coalfield in northern Shaanxi is mainly formed by the disturbance of roof aquifer by mining water diversion fracture zone, which can form a continuous large flow water gushing through sandstone aquifer or loose sand layer, and the water filling intensity is large in some areas. Roof flood occurs when the amount of water exceeds the capacity of the drainage system in a short time, and it can be transformed into water and sand burst disaster when the collapse zone in the thin bedrock area directly leads to the loose sand layer. When the water-conducting fracture zone affects the water-rich area of burnt rock, it can form a flood of burnt rock with large instantaneous water volume and continuous flood. The super-thick sandstone aquifer on the roof of the Huanglong coalfield is swept by the mining water-conducting fracture zone to form a high-intensity continuous water gushing and may form roof flood. When the thickness of the lower aquifer is large, the separated layer water burst disaster may be formed. When the roof strata near the coal seam is soft and easy to collapse, it can be transformed into mud and sand burst disaster. In the Carboniferous-Permian coalfield and the Weibei coalfield in northern Shaanxi, when structures such as coal seam mining floor disturbance failure zones or faults lead to the Ordovician limestone aquifers, they will cause extremely serious floor water inrush disasters, which are characterized by strong sudden occurrence and large instantaneous water volume. ③ The roof water disaster treatment technology in each region mainly includes drilling hole drainage, grouting treatment, mining parameter control and so on, and the floor water damage is mainly treated by regional grouting reinforcement and plugging. Burnt rock water disaster is mainly treated by the combination of curtain grouting and drilling exploration

miR-494 Sensitizes Gastric Cancer Cells to TRAIL Treatment Through Downregulation of Survivin

Background/Aims: TNF-related apoptosis-inducing ligand (TRAIL) is a novel and low-toxic anti-tumor drug used for various cancers. However, cancer cells usually develop mechanisms to acquire the resistance against TRAIL. Among these changes, dysregulation of microRNAs (miRNAs) usually occurs in cancer cells and is responsible for induction of drug resistance. Methods: Expression of miR-494 in gastric cancer tissues and cell lines was detected by quantitative reverse transcriptase real time PCR (qRT-PCR) analysis. Effect of miR-494 on regulating the TRAIL sensitivity to gastric cancer cell lines was evaluated by MTT assays. Bioinformatics and luciferase reporter assays were used to confirm the regulation of miR-494 on survivin. Mitochondrial apoptosis pathway in gastric cancer cells was tested by western blot and flow cytometry analysis. Results: Obvious downregulation of miR-494 was observed in gastric cancer cells. Furthermore, we found that expression profile of miR-494 was associated with TRAIL-sensitivity in gastric cancer. Enforced expression of miR-494 was found to sensitize the gastric cancer cells to TRAIL-induced cytotoxicity. Mechanically, Luciferase reporter assays proved that survivin was the target of miR-494 in gastric cancer cells. Enforced expression of miR-494 decreased the expression of survivin, and thus promoted the TRAIL-induced mitochondria collapse and apoptosis pathway. Conclusion: MiR-494/survivin axis represents a potential mechanism which is responsible for TRAIL resistance in gastric cancer cells. Increasing the miR-494 expression may serve as a novel therapeutic strategy to sensitize gastric cancer cells to TRAIL treatment

SRSF5‐Mediated Alternative Splicing of M Gene is Essential for Influenza A Virus Replication: A Host‐Directed Target Against Influenza Virus

Abstract: Splicing of influenza A virus (IAV) RNA is an essential process in the viral life cycle that involves the co‐opting of host factors. Here, it is demonstrated that induction of host serine and arginine‐rich splicing factor 5 (SRSF5) by IAV facilitated viral replication by enhancing viral M mRNA splicing. Mechanistically, SRSF5 with its RRM2 domain directly bounds M mRNA at conserved sites (M mRNA position 163, 709, and 712), and interacts with U1 small nuclear ribonucleoprotein (snRNP) to promote M mRNA splicing and M2 production. Mutations introduced to the three binding sites, without changing amino acid code, significantly attenuates virus replication and pathogenesis in vivo. Likewise, SRSF5 conditional knockout in the lung protects mice against lethal IAV challenge. Furthermore, anidulafungin, an approved antifungal drug, is identified as an inhibitor of SRSF5 that effectively blocks IAV replication in vitro and in vivo. In conclusion, SRSF5 as an activator of M mRNA splicing promotes IAV replication and is a host‐derived antiviral target

Pharmacokinetics, distribution, metabolism, and excretion of body-protective compound 157, a potential drug for treating various wounds, in rats and dogs

Body-protective compound (BPC) 157 demonstrates protective effects against damage to various organs and tissues. For future clinical applications, we had previously established a solid-phase synthesis process for BPC157, verified its biological activity in different wound models, and completed preclinical safety evaluations. This study aimed to investigate the pharmacokinetics, excretion, metabolism, and distribution profiles of BPC157. After a single intravenous (IV) administration, single intramuscular (IM) administrations at three doses in successive increments along with repeated IM administrations, the elimination half-life (t1/2) of prototype BPC157 was less than 30 min, and BPC157 showed linear pharmacokinetic characteristics in rats and beagle dogs at all doses. The mean absolute bioavailability of BPC157 following IM injection was approximately 14%–19% in rats and 45%–51% in beagle dogs. Using [3H]-labeled BPC157 and radioactivity examination, we proved that the main excretory pathways of BPC157 involved urine and bile. [3H]BPC157 was rapidly metabolized into a variety of small peptide fragments in vivo, thus forming single amino acids that entered normal amino acid metabolism and excretion pathways. In conclusion, this study provides the first analysis of the pharmacokinetics of BPC157, which will be helpful for its translation in the clinic

Protein-metabolite association studies identify novel proteomic determinants of metabolite levels in human plasma

Although many novel gene-metabolite and gene-protein associations have been identified using high-throughput biochemical profiling, systematic studies that leverage human genetics to illuminate causal relationships between circulating proteins and metabolites are lacking. Here, we performed protein-metabolite association studies in 3,626 plasma samples from three human cohorts. We detected 171,800 significant protein-metabolite pairwise correlations between 1,265 proteins and 365 metabolites, including established relationships in metabolic and signaling pathways such as the protein thyroxine-binding globulin and the metabolite thyroxine, as well as thousands of new findings. In Mendelian randomization (MR) analyses, we identified putative causal protein-to-metabolite associations. We experimentally validated top MR associations in proof-of-concept plasma metabolomics studies in three murine knockout strains of key protein regulators. These analyses identified previously unrecognized associations between bioactive proteins and metabolites in human plasma. We provide publicly available data to be leveraged for studies in human metabolism and disease

Shuning Guo's Quick Files

The Quick Files feature was discontinued and it’s files were migrated into this Project on March 11, 2022. The file URL’s will still resolve properly, and the Quick Files logs are available in the Project’s Recent Activity

A Glimpse into Humoral Response and Related Therapeutic Approaches of Takayasu’s Arteritis

Takayasu’s arteritis (TAK) manifests as an insidiously progressive and debilitating form of granulomatous inflammation including the aorta and its major branches. The precise etiology of TAK remains elusive, with current understanding suggesting an autoimmune origin primarily driven by T cells. Notably, a growing body of evidence bears testimony to the widespread effects of B cells on disease pathogenesis and progression. Distinct alterations in peripheral B cell subsets have been described in individuals with TAK. Advancements in technology have facilitated the identification of novel autoantibodies in TAK. Moreover, emerging data suggest that dysregulated signaling cascades downstream of B cell receptor families, including interactions with innate pattern recognition receptors such as toll-like receptors, as well as co-stimulatory molecules like CD40, CD80 and CD86, may result in the selection and proliferation of autoreactive B cell clones in TAK. Additionally, ectopic lymphoid neogenesis within the aortic wall of TAK patients exhibits functional characteristics. In recent decades, therapeutic interventions targeting B cells, notably utilizing the anti-CD20 monoclonal antibody rituximab, have demonstrated efficacy in TAK. Despite the importance of the humoral immune response, a systematic understanding of how autoreactive B cells contribute to the pathogenic process is still lacking. This review provides a comprehensive overview of the biological significance of B cell-mediated autoimmunity in TAK pathogenesis, as well as insights into therapeutic strategies targeting the humoral response. Furthermore, it examines the roles of T-helper and T follicular helper cells in humoral immunity and their potential contributions to disease mechanisms. We believe that further identification of the pathogenic role of autoimmune B cells and the underlying regulation system will lead to deeper personalized management of TAK patients. We believe that further elucidation of the pathogenic role of autoimmune B cells and the underlying regulatory mechanisms holds promise for the development of personalized approaches to managing TAK patients

The complete chloroplast genome of Polygala japonica Houtt. (Polygalaceae), a medicinal plant in China

Polygala japonica Houtt. (Polygalaceae) is a perennial herbaceous plant widely distributed in south China. Here, we assembled and characterized the complete chloroplast (cp) genome of P. japonica using sequencing data. The complete cp genome of P. japonica was 165,439 bp in length, with a GC content of 36.7%. The complete cp genome shows a typical quadripartite structure with a pair of inverted repeats (IRs) of 36,786 bp, separated by a large single copy region (LSC) of 83,722 bp, and a small single copy region (SSC) of 8,145 bp. A total of 135 genes were annotated in the cp genome of P. japonica, consisting of 89 protein-coding genes, 8 rRNA genes, and 38 tRNA genes. The phylogenetic analysis showed P. japonica had a closer relationship with P. tenuifolia