Relation Between Gravitational Mass and Baryonic Mass for Non-Rotating and Rapidly Rotating Neutron Stars

With a selected sample of neutron star (NS) equations of state (EOSs) that are consistent with the current observations and have a range of maximum masses, we investigate the relations between NS gravitational mass Mg and baryonic mass Mb, and the relations between the maximum NS mass supported through uniform rotation (Mmax) and that of nonrotating NSs (MTOV). We find that for an EOS-independent quadratic, universal transformation formula (Mb=Mg+A×M2g)(Mb=Mg+A×Mg2), the best-fit A value is 0.080 for non-rotating NSs, 0.064 for maximally rotating NSs, and 0.073 when NSs with arbitrary rotation are considered. The residual error of the transformation is ∼ 0.1M⊙ for non-spin or maximum-spin, but is as large as ∼ 0.2M⊙ for all spins. For different EOSs, we find that the parameter A for non-rotating NSs is proportional to R−11.4R1.4−1 (where R1.4 is NS radius for 1.4M⊙ in units of km). For a particular EOS, if one adopts the best-fit parameters for different spin periods, the residual error of the transformation is smaller, which is of the order of 0.01M⊙ for the quadratic form and less than 0.01M⊙ for the cubic form ((Mb=Mg+A1×M2g+A2×M3g)(Mb=Mg+A1×Mg2+A2×Mg3)). We also find a very tight and general correlation between the normalized mass gain due to spin Δm = (Mmax − MTOV)/MTOV and the spin period normalized to the Keplerian period PP, i.e., log10Δm=(−2.74±0.05)log10P+log10(0.20±0.01)log10Δm=(−2.74±0.05)log10P+log10(0.20±0.01), which is independent of EOS models. These empirical relations are helpful to study NS-NS mergers with a long-lived NS merger product using multi-messenger data. The application of our results to GW170817 is discussed

Formation of meso, N-diphenylprotoporphyrin IX by an aerobic reaction of phenylhydrazine with oxyhemoglobins.

Administration of phenylhydrazine to rabbits resulted in the denaturation of hemoglobins in erythrocytes, causing the formation of intracellular precipitates known as Heinz bodies, severe hemolytic anemia, and reticulocytosis. To elucidate the molecular mechanism of the destabilization, we allowed human oxyhemoglobins to react aerobically with phenylhydrazine. After treatment with acetic acid/HCl and H2SO4/methanol, the chloroform extract contained blue-green pigments of major products accompanied by different minor products. Each product was isolated by column chromatography. By fast-atom-bombardment mass spectrometry (FAB-MS) and proton nuclear magnetic resonance (1H-NMR) spectrometry, dimethyl esters of N-phenylprotoporphyrin IX and meso, N-diphenylprotoporphyrin IX were determined. Other major products also were determined to be dimethyl esters of triphenyl-and tetraphenyl-substituted protoporphyrins by FAB-MS. The formation of meso, N-diphenylprotoporphyrin indicated that the addition of a phenyl radical to the meso-carbon atom of the protoporphyrin ring occurred. Triphenyl and tetraphenyl adducts also indicated the formation of phenyl radicals in the aerobic reaction of phenylhydrazine with oxyhemoglobins. From these results, we suggest that the formation of phenyl radicals and the replacement of heme with phenyl-substituted protoporphyrins cause the destabilization of hemoglobins to induce Heinz bodies and hemolytic anemia with phenylhydrazine.</p

The Downregulation of the Expression of CD147 by Tumor Suppressor REIC/Dkk-3, and Its Implication in Human Prostate Cancer Cell Growth Inhibition

The cluster of differentiation 147 (CD147), also known as EMMPRIN, is a key molecule that promotes cancer progression. We previously developed an adenoviral vector encoding a tumor suppressor REIC/Dkk-3 gene (Ad-REIC) for cancer gene therapy. The therapeutic effects are based on suppressing the growth of cancer cells, but, the underlying molecular mechanism has not been fully clarified. To elucidate this mechanism, we investigated the effects of Ad-REIC on the expression of CD147 in LNCaP prostate cancer cells. Western blotting revealed that the expression of CD147 was significantly suppressed by Ad-REIC. Ad-REIC also suppressed the cell growth of LNCaP cells. Since other researchers have demonstrated that phosphorylated mitogen-activated protein kinases (MAPKs) and c-Myc protein positively regulate the expression of CD147, we investigated the correlation between the CD147 level and the activation of MAPK and c-Myc expression. Unexpectedly, no positive correlation was observed between CD147 and its possible regulators, suggesting that another signaling pathway was involved in the downregulation of CD147. This is the first study to show the downregulation of CD147 by Ad-REIC in prostate cancer cells. At least some of the therapeutic effects of Ad-REIC may be due to the downregulation of the cancer-progression factor, CD147

(7R,8S,9S,12S)-1-(4-Chloro­benz­yloxy)-13,14-didehydro-12-hy­droxy-2,13-dimeth­oxy-N-methyl­morphinane

The title compound, C26H30ClNO4, a sinomenine derivative, has five six-membered rings, two of which are aromatic, with a dihedral angle of 34.13 (20)° between these. The N-containing ring and the fourth ring exhibit chair conformations, while the fifth ring approximates an envelope conformation. A single inter­molecular O—H⋯N hydrogen-bonding inter­action gives a one-dimensional chain structure which extends along the a axis. The absolute configuration for the mol­ecule has been determined

UPLC-Q–TOF–MS, network analysis, and molecular docking to investigate the effect and active ingredients of tea-seed oil against bacterial pathogens

Object: This research intended to probe the antibacterial effect and pharmacodynamic substances of Tea-Seed Oil (TSO) through the use of ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) analysis, network analysis, and molecular docking.Methods: The major chemical components in the methanol-extracted fractions of TSO were subjected to UPLC-Q-TOF-MS. Network pharmacology and molecular docking techniques were integrated to investigate the core components, targets, and potential mechanisms of action through which the TSO exert their antibacterial properties. To evaluate the inhibitory effects, the minimum inhibitory concentration and diameter of the bacteriostatic circle were calculated for the potential active ingredients and their equal ratios of combinatorial components (ERCC) against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans. Moreover, the quantification of the active constituents within TSO was achieved through the utilization of high-performance liquid chromatography (HPLC).Results: The methanol-extracted fractions contained a total of 47 chemical components, predominantly consisting of unsaturated fatty acids and phenolic compounds. The network pharmacology analysis and molecular docking analysis revealed that various components, including gallocatechin, gallic acid, epigallocatechin, theophylline, chlorogenic acid, puerarin, and phlorizin, have the ability to interact with critical core targets such as serine/threonine protein kinase 1 (AKT1), epidermal growth factor receptor (EGFR), a monoclonal antibody to mitogen-activated protein kinase 14 (MAPK14), HSP90AA1, and estrogen receptor 1 (ESR1). Furthermore, these components can modulate the phosphatidylinositol-3-kinase protein kinase B (PI3K-AKT), estrogen, MAPK and interleukin 17 (IL-17) signaling pathways, hereby exerting antibacterial effects. In vitro validation trials have found that seven components, namely gallocatechin, gallic acid, epigallocatechin, theophylline, chlorogenic acid, puerarin, and phloretin, displayed substantial inhibitory effects on E. coli, S. aureus, P. aeruginosa, and C. albicans, and are typically present in tea oil, with a total content ranging from 15.87∼24.91 μg·g−1.Conclusion: The outcomes of this investigation possess the possibility to expand our knowledge base concerning the utilization of TSO, furnish a theoretical framework for the exploration of antibacterial drugs and cosmetics derived from inherently occurring TSO, and establish a robust groundwork for the advancement and implementations of TOS products within clinical settings

Fast Radio Bursts as Strong Waves Interacting with the Ambient Medium

Fast radio bursts (FRBs) are mysterious radio transients whose physical origin is still unknown. Within a few astronomical units near an FRB source, the electric field of the electromagnetic wave is so large that the electron oscillation velocity becomes relativistic, which makes the classical Thomson scattering theory and the linear plasma theory invalid. We discuss FRBs as strong waves interacting with the ambient medium, in terms of both electron motion properties and plasma properties. Several novel features are identified. (1) The cross section of Thomson scattering is significantly enhanced for the scattering photons. (2) On the other hand, because of the nonlinear plasma properties in strong waves, the near-source plasma is more transparent and has a smaller effective dispersion measure (DM) contribution to the observed value. For a repeating FRB source, the brighter bursts would have somewhat smaller DMs contributed by the near-source plasma. (3) The radiation beam undergoes relativistic self-focusing in a dense plasma, the degree of self-focusing (or squeezing) depends on the plasma density. Such a squeezing effect would affect the collimation angle and the true event rate of FRBs. (4) When an FRB propagates in a nearby ambient plasma, a wakefield wave in the plasma will be generated by the ponderomotive force of the FRB, and accelerates electrons in the ambient medium. However, such an effect is too weak to be observationally interesting

Testing the Hypothesis of Compact-binary-coalescence Origin of Fast Radio Bursts Using a Multimessenger Approach

In the literature, compact binary coalescences (CBCs) have been proposed as one of the main scenarios to explain the origin of some non-repeating fast radio bursts (FRBs). The large discrepancy between the FRB and CBC event rate densities suggests that their associations, if any, should only apply at most for a small fraction of FRBs. Through a Bayesian estimation method, we show how a statistical analysis of the coincident associations of FRBs with CBC gravitational wave (GW) events may test the hypothesis of these associations. We show that during the operation period of the advanced Laser Interferometer Gravitational-Wave Observatory (aLIGO), the detection of ~100 (~1000) GW-less FRBs with dispersion measure (DM) values smaller than 500 pc cm−3 could reach the constraint that less than 10% (or 1%) FRBs are related to binary black hole (BBH) mergers. The same number of FRBs with DM values smaller than 100 pc cm−3 is required to reach the same constraint for binary neutron star (BNS) mergers. With the upgrade of GW detectors, the same constraints for BBH and BNS mergers can be reached with fewer FRBs or looser requirements for the DM values. It is also possible to pose constraints on the fraction of each type of CBCs that are able to produce observable FRBs based on the event density of FRBs and CBCs. This would further constrain the dimensionless charge of black holes (BHs) in binary BH systems

Meta-analysis of antiviral protection of white spot syndrome virus vaccine to the shrimp

Currently, white spot syndrome virus (WSSV) is one of the most serious pathogens that impacts shrimp farming around the world. A WSSV vaccine provides a significant protective benefit to the host shrimp. Although various types of vaccines against WSSV have emerged, the immune effects among them were not compared, and it remains unclear which type of vaccine has the strongest protective effect. Meanwhile, due to the lack of effective routes of administration and immunization programs, WSSV vaccines have been greatly limited in the actual shrimp farming. To answer these questions, this study conducted a comprehensive meta-analysis over dozens of studies and compared all types WSSV vaccines, which include sub-unit protein vaccines, whole virus inactivated vaccines, DNA vaccines and RNA-based vaccines. The results showed that the RNA-based vaccine had the highest protection rate over the other three types of vaccines. Among the various sub-unit protein vaccines, VP26 vaccine had the best protective effects than other sub-unit protein vaccines. Moreover, this study demonstrated that vaccines expressed in eukaryotic hosts had higher protection rates than that of prokaryotic systems. Among the three immunization modes (oral administration, immersion and injection) used in monovalent protein vaccines, oral administration had the highest protection rate. In natural conditions, shrimp are mostly infected by the virus orally. These results provide a guide for exploration of a novel WSSV vaccine and help facilitate the application of WSSV vaccines in shrimp farming

CaM kinase Iα–induced phosphorylation of Drp1 regulates mitochondrial morphology

Mitochondria are dynamic organelles that frequently move, divide, and fuse with one another to maintain their architecture and functions. However, the signaling mechanisms involved in these processes are still not well characterized. In this study, we analyze mitochondrial dynamics and morphology in neurons. Using time-lapse imaging, we find that Ca2+ influx through voltage-dependent Ca2+ channels (VDCCs) causes a rapid halt in mitochondrial movement and induces mitochondrial fission. VDCC-associated Ca2+ signaling stimulates phosphorylation of dynamin-related protein 1 (Drp1) at serine 600 via activation of Ca2+/calmodulin-dependent protein kinase Iα (CaMKIα). In neurons and HeLa cells, phosphorylation of Drp1 at serine 600 is associated with an increase in Drp1 translocation to mitochondria, whereas in vitro, phosphorylation of Drp1 results in an increase in its affinity for Fis1. CaMKIα is a widely expressed protein kinase, suggesting that Ca2+ is likely to be functionally important in the control of mitochondrial dynamics through regulation of Drp1 phosphorylation in neurons and other cell types