Factors Controlling Sludge Density During Acid Mine Drainage Neutralization

This study was supported in part by the Office of Water Resources Research, U.S. Department of Interior under Project A-023-OHIO(print) iii, 156 p. : ill. ; 28 cmList of Figures -- List of Tables -- Summary of Major Conclusions -- Introduction -- Related Literature -- Section I - Equipment and Experimental Procedure -- Section II - Formation of Magnetic Sludges -- Section III - Formation of Dense Settled Sludge by Neutralization of Synthetic Acid Mine Drainage with Calcium Hydroxide and Sludge "Recirculation" -- Section IV - Formation of Dense Settled Sludge by Neutralization of Synthetic Acid Mine Drainage with Calcium Carbonate and Sludge "Recirculation" -- Acknowledgements -- Reference

Development of a Natural Laboratory for the Study of Acid Mine Drainage

A small drift mine in Vinton County, Ohio, has been characterized and instrumented to permit a detailed study of the influence of oxygen concentration, microgiological factors, and hydrologic features on rate of pyrite oxidation within the system. This experimental mine will be treated as a pilot-scale reactor to obtain information in a natural environment on the kinetics of the reaction responsible for acid mine drainage. Six observation wells were dug and core samples taken at selected points surrounding the mine to establish ground water table and the aquifer supplying the mine. Base rate data of acid run-off as a function of water flow rate and water level in observation wells were collected. The mine was sealed and maintained under a slight positive pressure of nitrogen. Oxygen concentration dropped to less that 2 % in three months. When "sealed" in a conventional manner, the atmosphere in the mine never dropped below 10 % oxygen after being closed for several years. Acid run-off decreased approximately 50 % after six months under nitrogen pressure. The response time of the mine to imposed changes and characterization of reaction sites, in terms of physical and chemical environment, as well as location, are now being studied

The Performance Profile of a Division I Football Team

Reporting athletic performance characteristics of a Division I collegiate football team may serve to inform coaching personnel and sports performance staff about the most versatile players to utilize for various competitions. Performance data may also help guide developing athletes about benchmarks to achieve for optimal competitiveness. PURPOSE: Our aim was to observe athletic performance characteristics of and differences between designated starters (ST) vs non-starters (NST). METHODS: 44 athletes – ST (n=22) vs NST (n=22) – were selected using a convenience sample and equally distributed between offense and defense positions. Preseason performance data were analyzed related to select power, including counter movement jump (CMJ), vertical jump (VJ), and Nordic hamstring (NH) tests; and select strength assessments, including 1 repetition max (RM) on power clean (PC), front squat (FS), and bench press (BP). All tests were conducted by sport performance staff using free weights and VALD Performance (Queensland, Australia) equipment (i.e., NordBord, ForceDecks) and software. Data were analyzed via a two sampled equal variance, right-tailed t-test with a significance level set at p \u3c 0.05 and reported as mean±SD. RESULTS: The average age, height, and weight for ST vs. NST, respectively, were 21.2±1.4 & 20.4±1.0 y, 1.8±.4 & 1.9±.1 m, and 104.3±32.1 & 100.4±20.0 kg. Power related to CMJ peak, VJ, NH max of right + left leg averaged, and NH impulse between ST vs. NST were, respectively: 62±9.3 vs. 67.5±12.4 W/kg (p=0.053), .7±.1 vs. .7±.1 m (p=0.444), 553.1±61.3 vs. 478.0±142.1 N (p=0.017), and 7982.1±2235.6 vs 6833.8±1952.4 NS (p=0.040). PC, FS, and BP for ST vs NST were, respectively: 133.4±13.8 vs. 122.4±10.9 kg (p=0.004), 164.5±23.5 vs. 146.7±38.2 kg (p=0.049), and 140.8±20.7 vs. 132.9±15.9 kg (p=0.095). CONCLUSION: Specific to the group observed, there appears to be select power and strength performance characteristics that separate ST and NST. ST might be advised to maintain and NST should strive to achieve explosiveness and strength as described herein via augmented bilateral NH force and impulse in addition to developing a high 1 RM PC. Despite the ambiguity of athletic performance tests to identify talent and skill, coaches and players still might be able to utilize these standards for individualized success

Three-D multilateration: A precision geodetic measurement system

A technique of satellite geodesy for determining the relative three dimensional coordinates of ground stations within one centimeter over baselines of 20 to 10,000 kilometers is discussed. The system is referred to as 3-D Multilateration and has applications in earthquake hazard assessment, precision surveying, plate tectonics, and orbital mechanics. The accuracy is obtained by using pulsed lasers to obtain simultaneous slant ranges between several ground stations and a moving retroreflector with known trajectory for aiming the lasers

Ion mobility spectrometry for the rapid analysis of over-the-counter drugs and beverages

In the pharmaceutical industry, there are increasing requirements for analytical methods in quality assessment for the production of drugs. In this investigation, ion mobility spectrometry (IMS) was used for the rapid qualitative separation and identification of active ingredients in generic over-the-counter drugs and food additives in beverages. The active ingredients determined in drugs were acetaminophen, aspartame, bisacodyl, caffeine, dextromethorphan, diphenhydramine, famotidine, glucosamine, guaifenesin, loratadine, niacin, phenylephrine, pyridoxine, thiamin, and tetrahydrozoline. Aspartame and caffeine were determined in beverages. Fourteen over-the-counter drugs and beverages were analyzed. Analysis times below 10 s were obtained for IMS, and reduced mobilities were reported for the first time for 12 compounds. A quadrupole mass spectrometer coupled to a mobility spectrometer was used to assure a correct peak assignation. The combination of fast analysis, low cost, and inexpensive maintenance of IMS instruments makes IMS an attractive technique for the qualitative determination of the active ingredients in over-the-counter drugs and food additives in manufacture quality control and cleaning verification for the drug and food industries

Diverging Mechanisms of Activation of Chemokine Receptors Revealed by Novel Chemokine Agonists

CXCL8/interleukin-8 is a pro-inflammatory chemokine that triggers pleiotropic responses, including inflammation, angiogenesis, wound healing and tumorigenesis. We engineered the first selective CXCR1 agonists on the basis of residue substitutions in the conserved ELR triad and CXC motif of CXCL8. Our data reveal that the molecular mechanisms of activation of CXCR1 and CXCR2 are distinct: the N-loop of CXCL8 is the major determinant for CXCR1 activation, whereas the N-terminus of CXCL8 (ELR and CXC) is essential for CXCR2 activation. We also found that activation of CXCR1 cross-desensitized CXCR2 responses in human neutrophils co-expressing both receptors, indicating that these novel CXCR1 agonists represent a new class of anti-inflammatory agents. Further, these selective CXCR1 agonists will aid at elucidating the functional significance of CXCR1 in vivo under pathophysiological conditions

Semi-automated assembly of high-quality diploid human reference genomes

The current human reference genome, GRCh38, represents over 20 years of effort to generate a high-quality assembly, which has benefitted society. However, it still has many gaps and errors, and does not represent a biological genome as it is a blend of multiple individuals. Recently, a high-quality telomere-to-telomere reference, CHM13, was generated with the latest long-read technologies, but it was derived from a hydatidiform mole cell line with a nearly homozygous genome. To address these limitations, the Human Pangenome Reference Consortium formed with the goal of creating high-quality, cost-effective, diploid genome assemblies for a pangenome reference that represents human genetic diversity. Here, in our first scientific report, we determined which combination of current genome sequencing and assembly approaches yield the most complete and accurate diploid genome assembly with minimal manual curation. Approaches that used highly accurate long reads and parent-child data with graph-based haplotype phasing during assembly outperformed those that did not. Developing a combination of the top-performing methods, we generated our first high-quality diploid reference assembly, containing only approximately four gaps per chromosome on average, with most chromosomes within ±1% of the length of CHM13. Nearly 48% of protein-coding genes have non-synonymous amino acid changes between haplotypes, and centromeric regions showed the highest diversity. Our findings serve as a foundation for assembling near-complete diploid human genomes at scale for a pangenome reference to capture global genetic variation from single nucleotides to structural rearrangements