Establishment of Callus and Cell Suspension Cultures of Helicteres isora L.

Helicteres isora L. (Malvaceae) is a medicinal plant highly used in traditional therapeutic practices. It has shown wide-spectrum therapeutic activities including anti-plasmodial, hypoglycemic, hypolipidemic, hepatoprotective, antinociceptive, antioxidant and anti-HIV. Present investigation was undertaken with an objective of establishment of cell suspension cultures of this plant which can be further used for in vitro production of desired secondary metabolites and their further scale-up. Seed dormancy was broken using sulfuric acid and seedlings were raised in vitro. MS medium supplemented with 2,4-D (0.5 mg/L) produced maximum callus from the nodal explants. The callus produced was used as an explant for the establishment of suspension cultures. MS medium without any supplement was proved best for the establishment of cell suspension cultures of H. isora. To the best of our knowledge, this is the first report on H. isora cell suspension culture establishment.   &nbsp

Inhibiting Bacterial Drug Efflux Pumps via Phyto-Therapeutics to Combat Threatening Antimicrobial Resistance

Antibiotics, once considered the lifeline for treating bacterial infections, are under threat due to the emergence of threatening antimicrobial resistance (AMR). These drug-resistant microbes (or superbugs) are non-responsive to most of the commonly used antibiotics leaving us with few treatment options and escalating mortality-rates and treatment costs. The problem is further aggravated by the drying-pipeline of new and potent antibiotics effective particularly against the drug-resistant strains. Multidrug efflux pumps (EPs) are established as principal determinants of AMR, extruding multiple antibiotics out of the cell, mostly in non-specific manner and have therefore emerged as potent drug-targets for combating AMR. Plants being the reservoir of bioactive compounds can serve as a source of potent EP inhibitors (EPIs). The phyto-therapeutics with noteworthy drug-resistance-reversal or re-sensitizing activities may prove significant for reviving the otherwise fading antibiotics arsenal and making this combination-therapy effective. Contemporary attempts to potentiate the antibiotics with plant extracts and pure phytomolecules have gained momentum though with relatively less success against Gram-negative bacteria. Plant-based EPIs hold promise as potent drug-leads to combat the EPI-mediated AMR. This review presents an account of major bacterial multidrug EPs, their roles in imparting AMR, effective strategies for inhibiting drug EPs with phytomolecules, and current account of research on developing novel and potent plant-based EPIs for reversing their AMR characteristics. Recent developments including emergence of in silico tools, major success stories, challenges and future prospects are also discussed

Find Thy Neighbourhood: Privacy-Preserving Local Clustering

Identifying a cluster around a seed node in a graph, termed local clustering, finds use in several applications, including fraud detection, targeted advertising, community detection, etc. However, performing local clustering is challenging when the graph is distributed among multiple data owners, which is further aggravated by the privacy concerns that arise in disclosing their view of the graph. This necessitates designing solutions for privacy-preserving local clustering and is addressed for the first time in the literature. We propose using the technique of secure multiparty computation (MPC) to achieve the same. Our local clustering algorithm is based on the heat kernel PageRank (HKPR) metric, which produces the best-known cluster quality. En route to our final solution, we have two important steps: (i) designing data-oblivious equivalent of the state-of-the-art algorithms for computing local clustering and HKPR values, and (ii) compiling the data-oblivious algorithms into its secure realisation via an MPC framework that supports operations over fixed-point arithmetic representation such as multiplication and division. Keeping efficiency in mind for large graphs, we choose the best-known honest-majority 3-party framework of SWIFT (Koti et al., USENIX\u2721) and enhance it with some of the necessary yet missing primitives, before using it for our purpose. We benchmark the performance of our secure protocols, and the reported run time showcases the practicality of the same. Further, we perform extensive experiments to evaluate the accuracy loss of our protocols. Compared to their cleartext counterparts, we observe that the results are comparable and thus showcase the practicality of the designed protocols

Ruffle: Rapid 3-party shuffle protocols

Secure shuffle is an important primitive that finds use in several applications such as secure electronic voting, oblivious RAMs, secure sorting, to name a few. For time-sensitive shuffle-based applications that demand a fast response time, it is essential to design a fast and efficient shuffle protocol. In this work, we design secure and fast shuffle protocols relying on the techniques of secure multiparty computation. We make several design choices that aid in achieving highly efficient protocols. Specifically, we consider malicious 3-party computation setting with an honest majority and design robust ring-based protocols. Our shuffle protocols provide a fast online (i.e., input-dependent) phase compared to the state-of-the-art for the considered setting. To showcase the efficiency improvements brought in by our shuffle protocols, we consider two distinct applications of anonymous broadcast and secure graph computation via the GraphSC paradigm. In both cases, multiple shuffle invocations are required. Hence, going beyond standalone shuffle invocation, we identify two distinct scenarios of multiple invocations and provide customised protocols for the same. Further, we showcase that our customized protocols not only provide a fast response time, but also provide improved overall run time for multiple shuffle invocations. With respect to the applications, we not only improve in terms of efficiency, but also work towards providing improved security guarantees, thereby outperforming the respective state-of-the-art works. We benchmark our shuffle protocols and the considered applications to analyze the efficiency improvements with respect to various parameters

Retrospective evaluation of whole exome and genome mutation calls in 746 cancer samples

Funder: NCI U24CA211006Abstract: The Cancer Genome Atlas (TCGA) and International Cancer Genome Consortium (ICGC) curated consensus somatic mutation calls using whole exome sequencing (WES) and whole genome sequencing (WGS), respectively. Here, as part of the ICGC/TCGA Pan-Cancer Analysis of Whole Genomes (PCAWG) Consortium, which aggregated whole genome sequencing data from 2,658 cancers across 38 tumour types, we compare WES and WGS side-by-side from 746 TCGA samples, finding that ~80% of mutations overlap in covered exonic regions. We estimate that low variant allele fraction (VAF < 15%) and clonal heterogeneity contribute up to 68% of private WGS mutations and 71% of private WES mutations. We observe that ~30% of private WGS mutations trace to mutations identified by a single variant caller in WES consensus efforts. WGS captures both ~50% more variation in exonic regions and un-observed mutations in loci with variable GC-content. Together, our analysis highlights technological divergences between two reproducible somatic variant detection efforts

Phytohormones and their metabolic engineering for abiotic stress tolerance in crop plants

Abiotic stresses including drought, salinity, heat, cold, flooding, and ultraviolet radiation causes crop losses worldwide. In recent times, preventing these crop losses and producing more food and feed to meet the demands of ever-increasing human populations have gained unprecedented importance. However, the proportion of agricultural lands facing multiple abiotic stresses is expected only to rise under a changing global climate fueled by anthropogenic activities. Identifying the mechanisms developed and deployed by plants to counteract abiotic stresses and maintain their growth and survival under harsh conditions thus holds great significance. Recent investigations have shown that phytohormones, including the classical auxins, cytokinins, ethylene, and gibberellins, and newer members including brassinosteroids, jasmonates, and strigolactones may prove to be important metabolic engineering targets for producing abiotic stress-tolerant crop plants. In this review, we summarize and critically assess the roles that phytohormones play in plant growth and development and abiotic stress tolerance, besides their engineering for conferring abiotic stress tolerance in transgenic crops. We also describe recent successes in identifying the roles of phytohormones under stressful conditions. We conclude by describing the recent progress and future prospects including limitations and challenges of phytohormone engineering for inducing abiotic stress tolerance in crop plants

Antibiotic pollution and associated antimicrobial resistance in the environment

The widespread and injudicious usage of antibiotics to contain pathogenic microbial infections, coupled with inadequate treatment of wastes containing non-metabolized antibiotics and their residues is resulting in rising environmental antibiotic concentrations, leading to ‘antibiotic pollution’. Antibiotic pollution is an emerging global challenge as it is proving a major driver for antibiotic- or antimicrobial-resistance (ABR/ AMR) with escalating ramifications worldwide, precipitating a surge in human morbidity. The extensive administration of antibiotics across domains such as human healthcare settings, agriculture, and aquaculture farming endangers the presence of antibiotics in diverse matrices including water, soil, and air. As a result, this dissemination significantly leads to the development of antibiotic resistance in the profuse sectors of the environment further provoking consequential health implications at different trophic levels. Owing to its significance, and to mitigate antibiotic pollution and its subsequential AMR, governmental guidelines and regulations are implemented across the globe to cultivate public awareness as concerted efforts for addressing this global predicament. Given the insufficient attention to the growing antibiotic pollution issues, prompt efforts must be taken to contemplate current circumstances and the rigor of the ongoing research. In this review, we endeavor to elucidate the escalation of antibiotic concentration and antibiotic-driven AMR in water, air, and soil environments with potential public health threats. Further, it focuses on various strategies and interventions to attenuate antibiotic pollution and mitigate its adverse impacts on the healthcare infrastructure, highlighting the success stories, challenges, and future directions

<i style="mso-bidi-font-style:normal">In vitro</i> regeneration and ploidy level analysis of <i style="mso-bidi-font-style:normal">Eulophia ochreata </i>Lindl

1112-1121Various parameters including explant-type, medium compositions, use of phytohormones and additives were optimized for direct and indirect regeneration of E. ochreata, a medicinal orchid under threat. Protocorm-like-bodies (PLBs) proved to be the best explants for shoot initiation, proliferation and callus induction. Murashige and Skoog’s (MS) medium containing 2.5 mg L-1 6-benzylaminopurine (BAP), 1.0 mg L-1 kinetin (Kin) and additives (adenine sulfate, arginine, citric acid, 30 mg L-1 each and 50 mg L-1 ascorbic acid) was optimal for shoot multiplication (12.1 shoots and 7.1 PLBs per explant with synchronized growth), which also produced callus. Shoot number was further increased with three successive subcultures on same media and ~40 shoots per explant were achieved after 3 cycles of 30 days each. Additives and casein hydrolysate (CH) showed advantageous effects on indirect shoot regeneration via protocorm-derived callus. Optimum indirect regeneration was achieved on MS containing additives, 500 mg L-1 CH, 2.5 mg L-1 BAP and 1.0 mg L-1 Kin with 30 PLBs and 6 shoots per callus mass (~5 mm size). The shoots were rooted (70% frequency) on one by fourth-MS medium containing 2.0 mg L-1 indole-3-butyric acid, 200 mg L-1 activated charcoal and additives. The rooted plantlets were hardened and transferred to greenhouse with 63% survival rate. Flow-cytometry based DNA content analysis revealed that the ploidy levels were maintained in <i style="mso-bidi-font-style: normal">in vitro regenerated plants. This is the first report for in vitro plant regeneration in E. ochreata.<i style="mso-bidi-font-style: normal"></i

Application of P-SVR Methodology for comparison of Postural Severity of various tasks on compressor assembly line

Abstract The scope of this paper is to describe the newly defined Posture -State Variation Report (P-SVR

A critical review on Nepal Dock (Rumex nepalensis): A tropical herb with immense medicinal importance

Rumex nepalensis Spreng. (Polygonaceae) commonly known as Nepal Dock has wide-spectrum therapeutic potencies and is extensively used for centuries in traditional medicine systems. The leaves of this plant are edible and a rich source of natural antioxidants. They act as a possible food supplement and are largely used in pharmaceutical industry. Extracts and metabolites from this plant exhibits pharmacological activities including anti-inflammatory, antioxidant, antibacterial, antifungal, antiviral, insecticidal, purgative, analgesic, antipyretic, anti-algal, central nervous system depressant, genotoxic, wound healing and skeletal muscle relaxant activity. Due to its remarkable biological activities, it has the potential to act as a rich source of drug against life threatening diseases. However, more studies are needed to scientifically validate the traditional uses of this plant, beside isolating and identifying their active principles and characterizing the mechanisms of action. We present herein a critical account of its botany, ecology, traditional uses, phytoconstituent profile and major pharmacological activities reported in recent years and therefore will provide a source of information on this plant for further studies