“…beads which have given rise to so much dogmatism, controversy and rash speculation”: faience in Early Bronze Age Britain and Ireland

This volume represents the publication of a highly successful conference held in 2003 to celebrate the contribution to Neolithic and Early Bronze Age studies of one of archaeology's finest synthesisers, Professor Stuart Piggott. The title is a reference to his famous work, Ancient Europe from the beginnings of agriculture to Classical Antiquity, itself a publication of his Society of Antiquaries of Scotland's Rhind Lectures of 1962. The scope of the volume spans the three crucial millennia, from the beginning of the fourth to the mid second, that saw major impacts on the area we now call Scotland. There was transformation of the landscape through the introduction and development of farming, the creation of many striking monuments and the spread of important ideas and technologies, of which metalworking, particularly in Aberdeenshire, was one of the most significant. The contributions cover major advances in research in the period which demonstrate the interplay of the key factors of climate, culture, and resources, where the theme of exchange of information, objects and materials played a vital role. Individual chapters range from chambered tombs to climate change, from dietary choices to faience beads, from timber enclosures to bronze hoards. Together these present a valuable and up-to-the-minute overview of Scotland in ancient Europe and a fine tribute to a past-master of the subject

The investigation and characterisation of colourless glass from forensic and archaeological contexts using multiple interdisiplinary analytical techniques

The techniques used to analyse glass in forensics and archaeology differ: forensic analysis relies almost completely on Refractive Index, whereas archaeology uses compositional analyses. This thesis focuses on examining, explaining and challenging those differences through the analysis of colourless glass from forensic and archaeological contexts. Two major studies are undertaken, one focussing on stained and painted glass from Christ Church Cathedral, Oxford, and one based on modern automotive glass. The analytical techniques common to each discipline are applied to the samples of glass used in the research. The studies each focus on individual research questions; the Christ Church study investigates the 17th century Van Linge window scheme, the compositions of fragments of glass from throughout the cathedral’s history are investigated, and shown to separate into distinct compositional groups. The study also successfully recreates the Van Linge window scheme; and the historical and compositional analyses allowed the study of one of the in-situ painted windows in the cathedral. The automotive glass study investigates the complex relationship between glass manufacturers and vehicle manufacturers. The research revealed that certain makes of vehicle were beginning to group and glass from specific areas of the world was also grouping together. This study highlights the potential use for glass as a tool for intelligence gathering rather than just as evidence. The application of the different analytical techniques to each study is discussed along with the merits of using each of the techniques. The potential of using forensic techniques in archaeology and archaeological techniques in forensics is evaluated. Specifically, LA-ICP-MS, which is rarely used in forensics, could be an innovative and potentially important tool for the forensic evaluation of cars and other glass evidence. A discussion of the research and databases needed to make the most of the techniques is assessed.EThOS - Electronic Theses Online ServiceGBUnited Kingdo

The investigation and characterisation of colourless glass from forensic and archaeological contexts using multiple interdisiplinary analytical techniques

The techniques used to analyse glass in forensics and archaeology differ: forensic analysis relies almost completely on Refractive Index, whereas archaeology uses compositional analyses. This thesis focuses on examining, explaining and challenging those differences through the analysis of colourless glass from forensic and archaeological contexts. Two major studies are undertaken, one focussing on stained and painted glass from Christ Church Cathedral, Oxford, and one based on modern automotive glass. The analytical techniques common to each discipline are applied to the samples of glass used in the research. The studies each focus on individual research questions; the Christ Church study investigates the 17th century Van Linge window scheme, the compositions of fragments of glass from throughout the cathedral’s history are investigated, and shown to separate into distinct compositional groups. The study also successfully recreates the Van Linge window scheme; and the historical and compositional analyses allowed the study of one of the in-situ painted windows in the cathedral. The automotive glass study investigates the complex relationship between glass manufacturers and vehicle manufacturers. The research revealed that certain makes of vehicle were beginning to group and glass from specific areas of the world was also grouping together. This study highlights the potential use for glass as a tool for intelligence gathering rather than just as evidence. The application of the different analytical techniques to each study is discussed along with the merits of using each of the techniques. The potential of using forensic techniques in archaeology and archaeological techniques in forensics is evaluated. Specifically, LA-ICP-MS, which is rarely used in forensics, could be an innovative and potentially important tool for the forensic evaluation of cars and other glass evidence. A discussion of the research and databases needed to make the most of the techniques is assessed.EThOS - Electronic Theses Online ServiceGBUnited Kingdo

Ancient Biographies: Trace element analysis to investigate provenance and transportation mechanism of Late Bronze Age glass

Volume 1, Issue 2 (2017), delayed publication 2019LA-ICPMS analysis was carried out on a scaraboid blue glass bead (Hunterian Museum Glasgow, D.1921.39) excavated from Tomb 27 in Gurob, in the Southern Fayum region of Egypt. Gurob is known to have been the site of a ‘harem palace’ established in the reign of Tuthmosis III (1479-1425 BC). The tomb was located at the northernmost point of the New Kingdom cemetery and was undisturbed, containing the remains of seven females and two children, and was dated by the excavators to between the reigns of Amenophis I (1525-1504 BC) and Tuthmosis III. The glass scarab was coloured by copper and trace element values of La, Cr, Ti and Zr exhibited compositional consistency with glasses from Mesopotamia, rather than from Egypt. Therefore, the glass scarab represents a rare example of Mesopotamian glass to be discovered in Egypt, in addition to being some of the earliest glass found. The finds support iconographic references in the Hall of the Annals at Karnak to the import of early glass into Egypt. The implication is that these beads represent luxury items transported into Egypt by high-ranking foreign women perhaps in connection with the Gurob harem palace

Radiocarbon dating of Early Egyptian pot residues

A number of absolute dating techniques are now used in archaeology, from dendrochronology to a variety of luminescence and radiometric methods.1 However, radiocarbon dating remains the most effective approach for the early historic periods. This is largely because of the levels of precision achievable, but also due to the diversity of materials that can be dated, and the ease with which radiocarbon dates can be connected to specific events in the past. Radiocarbon dating can be employed on all carbon-containing materials that are biogenic in origin. Common sample types include items fashioned from plant material, such as textiles and basketry, and the remains of animal and human tissue. Radiocarbon estimates denote the time elapsed since the antecedent organism ceased exchanging carbon with its environment. For human and animal remains this is invariably taken to be the time of death, and for plants it is most commonly the time at which the material was harvested or felled. With the advent of accelerator mass spectrometry (AMS) in the 1980s, it became possible to conduct radiocarbon analysis on samples several orders of magnitude smaller than preceding techniques.2 Typically, AMS can produce reliable dates on as little as 10 mg of plant material and just 250 mg of whole bone powder. As a result, AMS accounts for a large proportion of the dates made on archaeological samples. No form of radiocarbon dating can, however, provide direct estimates for the age of lithic or ceramic artefacts. The principle difficulty lies in relating any datable material obtained to the manufacture or use of the object in question. In fact, carbonaceous inclusions in such materials are likely to be of geological age, and therefore beyond the 50,000 year detection limit of the technique. Consequently, there remains a disjunction between radiocarbon results and dates based on ceramic seriation. One possibility at bridging this divide comes from the radiocarbon dating of organic residues adhered to specific ceramic types. This prospect was investigated for Early Egypt by an interdisciplinary research team from the University of Oxford, University College London and Cranfield University

Histological and top-down proteomic analyses of the visual pathway in the cuprizone demyelination model

A change in visual perception is a frequent early symptom of multiple sclerosis (MS), the pathoaetiology of which remains unclear. Following a slow demyelination process caused by 12 weeks of low-dose (0.1%) cuprizone (CPZ) consumption, histology and proteomics were used to investigate components of the visual pathway in young adult mice. Histological investigation did not identify demyelination or gliosis in the optic tracts, pretectal nuclei, superior colliculi, lateral geniculate nuclei or visual cortices. However, top-down proteomic assessment of the optic nerve/tract revealed a significant change in the abundance of 34 spots in high-resolution two-dimensional (2D) gels. Subsequent liquid chromatography-tandem mass spectrometry (LC-TMS) analysis identified alterations in 75 proteoforms. Literature mining revealed the relevance of these proteoforms in terms of proteins previously implicated in animal models, eye diseases and human MS. Importantly, 24 proteoforms were not previously described in any animal models of MS, eye diseases or MS itself. Bioinformatic analysis indicated involvement of these proteoforms in cytoskeleton organization, metabolic dysregulation, protein aggregation and axonal support. Collectively, these results indicate that continuous CPZ-feeding, which evokes a slow demyelination, results in proteomic changes that precede any clear histological changes in the visual pathway and that these proteoforms may be potential early markers of degenerative demyelinating conditions

LA-ICP-MS analysis of late bronze age blue glass beads from Gurob, Egypt

LA-ICP-MS analysis was undertaken on 37 blue glass beads excavated from a tomb in Gurob, in the Southern Fayum region of Egypt. The tomb was undisturbed, contained the remains of seven females and two children, and dated between the reigns of Amenhotep I (1525-1504 BC) and Tuthmosis III (1479-1425 BC). The glass beads are coloured by copper and the trace element concentrations are compositionally consistent with glasses from Mesopotamia, rather than from Egypt. Therefore, these glass beads represent a rare example of Mesopotamian glass to be discovered in Egypt, in addition to being some of the earliest glass found. Gurob is known to have been the site of a ‘harem palace’ established in the reign of Tuthmosis III (1479-1425 BC), the implication being that these beads represent luxury items transported into Egypt by high-ranking foreign women, possibly in connection with the harem palace

Proteomics of Multiple Sclerosis : inherent issues in defining the pathoetiology and identifying (early) biomarkers

Multiple Sclerosis (MS) is a demyelinating disease of the human central nervous system having an unconfirmed pathoetiology. Although animal models are used to mimic the pathology and clinical symptoms, no single model successfully replicates the full complexity of MS from its initial clinical identification through disease progression. Most importantly, a lack of preclinical biomarkers is hampering the earliest possible diagnosis and treatment. Notably, the development of rationally targeted therapeutics enabling pre‐emptive treatment to halt the disease is also delayed without such biomarkers. Using literature mining and bioinformatic analyses, this review assessed the available proteomic studies of MS patients and animal models to discern (1) whether the models effectively mimic MS; and (2) whether reasonable biomarker candidates have been identified. The implication and necessity of assessing proteoforms and the critical importance of this to identifying rational biomarkers are discussed. Moreover, the challenges of using different proteomic analytical approaches and biological samples are also addressed

CD8 T-cell recruitment into the central nervous system of cuprizone-fed mice : relevance to modelling the etiology of Multiple Sclerosis

Cuprizone (CPZ)-feeding in mice induces atrophy of peripheral immune organs (thymus and spleen) and suppresses T-cell levels, thereby limiting its use as a model for studying the effects of the immune system in demyelinating diseases such as Multiple Sclerosis (MS). To investigate whether castration (Cx) can protect the peripheral immune organs from CPZ-induced atrophy and enable T-cell recruitment into the central nervous system (CNS) following a breach of the blood-brain barrier (BBB), three related studies were carried out. In Study 1, Cx prevented the dose-dependent reductions (0.1% < 0.2% CPZ) in thymic and splenic weight, size of the thymic medulla and splenic white pulp, and CD4 and CD8 (CD4/8) levels remained comparable to gonadally intact (Gi) control males. Importantly, 0.1% and 0.2% CPZ were equipotent at inducing central demyelination and glial activation. In Study 2, combining Cx with 0.1% CPZ-feeding and BBB disruption with pertussis toxin (PT) enhanced CD8+ T-cell recruitment into the CNS. The increased CD8+ T-cell level observed in the parenchyma of the cerebrum, cerebellum, brainstem and spinal cord were confirmed by flow cytometry and western blot analyses of CNS tissue. In Study 3, PT+0.1% CPZ-feeding to Gi female mice resulted in similar effects on the peripheral immune organs, CNS demyelination, and gliosis comparable to Gi males, indicating that testosterone levels alone were not responsible for the immune response seen in Study 2. The combination of Cx+0.1% CPZ-feeding+PT indicates that CPZ-induced demyelination can trigger an “inside-out” immune response when the peripheral immune system is spared and may provide a better model to study the initiating events in demyelinating conditions such as MS