A Multiple Group Repeated Measures Confirmatory Factor Analysis (CFA) Examination of the Evolutionary Attitudes and Literacy Survey (EALS) Among College Samples

The present study examined changes in university students' attitudes toward and knowledge of evolution measured by the previously validated Evolutionary Attitudes and Literacy Survey (EALS). Students were assessed at a large Midwestern U. S. university prior to and following completion of either an undergraduate political science, biology, or evolutionary psychology course. A multiple group repeated measures confirmatory factor analysis (CFA) was conducted to examine latent mean differences in self-reported political activity, religious conservatism, evolution knowledge/relevance, creationist reasoning, evolutionary misconceptions, and exposure to evolution. A significant and notable increase in evolution knowledge/relevance, as well as decrease in creationist reasoning and evolutionary misconceptions was observed for the evolutionary psychology course. In contrast, no significant change in evolution knowledge/relevance was observed for the biology course. The implications of these findings, as well as limitations and future research for evolution education are discussed

Power of Alternative Fit Indices for Multiple Group Longitudinal Tests of Measurement Invariance

Measurement invariance testing with confirmatory factor analysis has a long history in social science research, and more recently has increased use and popularity. The current paper begins by reviewing the steps for measurement invariance testing via multiple group confirmatory factor analysis, and synthesizing previous research recommendations for model testing, including the chi-square difference test, and examining change in model fit indices. Previous research on measurement invariance testing has examined change in alternative fit indices such as the CFI, TLI, RMSEA, and SRMR, but these studies had not examined power to detect invariance when more than two groups exist and multiple time points are present. The present study implemented a Monte Carlo simulation to examine the power of change in alternative fit indices to detect two types of measurement invariance, weak and strong, across a variety of manipulated study conditions including sample size, sample size ratio, lack of invariance, location of noninvariance, magnitude of noninvariance, and type of mixed study design

Evolutionary attitudes and literacy survey (EALS): Development and validation of a short form

This is the publisher's version, also available electronically from http://link.springer.com/article/10.1007%2Fs12052-012-0429-7.The Evolutionary Attitudes and Literacy Survey (EALS) is a multidimensional scale consisting of 16 lower- and 6 higher-order constructs developed to measure the wide array of factors that influence both an individual’s endorsement of and objection to evolutionary theory. Past research has demonstrated the validity and utility of the EALS (Hawley et al., Evol Educ Outreach 4:117–132, 2011); however, the 104-item long-form scale may be excessive for researchers and educators. The present study sought to reduce the number of items in the EALS while maintaining the validity and structure of the long form. For the present study, and following best practices for short-form construction, we surveyed a new sample of several hundred undergraduates from multiple majors and reduced the long form by 40% while maintaining the scale structure and validity. A multiple-group confirmatory factor analysis supported strong factorial invariance across samples, and therefore verified structure and pattern between the six higher-order constructs of the long-form EALS and the EALS short form (EALS-SF). Regression analysis further demonstrated the short form’s validity (i.e., demographics and openness to experience) and replicated previous findings. In the end, the EALS-SF may be a versatile tool that may be used whole or in part for a variety of research areas, including curricular effectiveness of courses on evolution and/or biology

What's the Matter with Kansas?: The Development and Confirmation of the Evolutionary Attitudes and Literacy Survey (EALS)

This is the publisher's version, also available electronically from http://link.springer.com/article/10.1007%2Fs12052-010-0294-1.The present survey was designed to assess predominant regional belief systems and the roles these beliefs play in science understanding and attitudes, and curricular effectiveness in colleges and universities. To this end, we created a wide variety of theory-driven subscales (lower order factors) reflecting, for example, exposure to evolutionary material, young earth creationist beliefs, moral and social objections, political ideology, endorsement of intelligent design fallacies, knowledge (and distrust) of the scientific enterprise, and attitudes of evolutionary theory’s relevance in several domains (e.g., sciences and humanities). We also included potentially important demographic variables (e.g., rural upbringing, family size). Finally, we assessed openness to experience, a key facet of personality. Hierarchical Structural Equation Modeling (SEM) analysis showed the 16 constructs to have a theoretically meaningful and quantitatively coherent higher order factor structure. In this large Kansas sample of university students, creationist reasoning and conservative orientation (political and religious) were negatively associated with exposure to evolutionary theory, knowledge about it, and positive attitudes toward its relevance. At the same time, exposure to the theory was positively associated with knowledge and positive attitudes. Importantly, though most Kansas-specific demographic variables (e.g., rural origins) were largely unrelated to outcomes of interest in this university-based sample, the personality factor openness to experience appears to be highly relevant for several higher order factors (e.g., exposure, knowledge and relevance, and creationist reasoning). We close with implications for educators and the next steps in survey development

What's the Matter with Kansas?: The Development and Confirmation of the Evolutionary Attitudes and Literacy Survey (EALS)

This is the publisher's version, also available electronically from http://link.springer.com/article/10.1007%2Fs12052-010-0294-1.The present survey was designed to assess predominant regional belief systems and the roles these beliefs play in science understanding and attitudes, and curricular effectiveness in colleges and universities. To this end, we created a wide variety of theory-driven subscales (lower order factors) reflecting, for example, exposure to evolutionary material, young earth creationist beliefs, moral and social objections, political ideology, endorsement of intelligent design fallacies, knowledge (and distrust) of the scientific enterprise, and attitudes of evolutionary theory’s relevance in several domains (e.g., sciences and humanities). We also included potentially important demographic variables (e.g., rural upbringing, family size). Finally, we assessed openness to experience, a key facet of personality. Hierarchical Structural Equation Modeling (SEM) analysis showed the 16 constructs to have a theoretically meaningful and quantitatively coherent higher order factor structure. In this large Kansas sample of university students, creationist reasoning and conservative orientation (political and religious) were negatively associated with exposure to evolutionary theory, knowledge about it, and positive attitudes toward its relevance. At the same time, exposure to the theory was positively associated with knowledge and positive attitudes. Importantly, though most Kansas-specific demographic variables (e.g., rural origins) were largely unrelated to outcomes of interest in this university-based sample, the personality factor openness to experience appears to be highly relevant for several higher order factors (e.g., exposure, knowledge and relevance, and creationist reasoning). We close with implications for educators and the next steps in survey development

The genome sequence of the red compost earthworm, Lumbricus rubellus (Hoffmeister, 1843)

We present a genome assembly from an individual Lumbricus rubellus (the red compost earthworm; Annelida; Clitellata; Haplotaxida; Lumbricidae). The genome sequence is 787.5 megabases in span. Most of the assembly is scaffolded into 18 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 15.81 kilobases in length. Gene annotation of this assembly on Ensembl identified 33,426 protein coding genes

The genome sequence of the cabbage moth, Mamestra brassicae (Linnaeus, 1758)

We present a genome assembly from an individual male Mamestra brassicae (the Cabbage Moth; Arthropoda; Insecta; Lepidoptera; Noctuidae). The genome sequence is 576.2 megabases in span. Most of the assembly is scaffolded into 31 chromosomal pseudomolecules, including the Z sex chromosome. The mitochondrial genome has also been assembled and is 15.38 kilobases in length. Gene annotation of this assembly on Ensembl identified 12,891 protein coding genes

Off-target stoichiometric binding identified from toxicogenomics explains why some species are more sensitive than others to a widely used neonicotinoid

Neonicotinoids are currently licensed for use in 120 countries, making accurate nontarget species sensitivity predictions critical. Unfortunately, such predictions are fraught with uncertainty, as sensitivity is extrapolated from only a few test species and neonicotinoid sensitivities can differ greatly between closely related taxa. Combining classical toxicology with de novo toxicogenomics could greatly improve sensitivity predictions and identify unexpectedly susceptible species. We show that there is a >30-fold differential species sensitivity (DSS) for the neonicotinoid imidacloprid between five earthworm species, a critical nontarget taxon. This variation could not be explained by differential toxicokinetics. Furthermore, comparing key motif expression in subunit genes of the classical nicotinic acetylcholine receptor (nAChR) target predicts only minor differences in the ligand binding domains (LBDs). In contrast, predicted dissimilarities in LBDs do occur in the highly expressed but nonclassical targets, acetylcholine binding proteins (AChBPs). Critically, the predicted AChBP divergence is capable of explaining DSS. We propose that high expression levels of putative nonsynaptic AChBPs with high imidacloprid affinities reduce imidacloprid binding to critical nAChRs involved in vital synaptic neurotransmission. This study provides a clear example of how pragmatic interrogation of key motif expression in complex multisubunit receptors can predict observed DSS, thereby informing sensitivity predictions for essential nontarget species

Molecular insights into high-altitude adaption and acclimatisation of Aporrectodea caliginosa

Here, we explore the high-altitude adaptions and acclimatisation of Aporrectodea caliginosa. Population diversity is assessed through mitochondrial barcoding, identifying closely related populations across the island of Pico (Azores). We present the first megabase N50 assembly size (1.2 Mbp) genome for A. caliginosa. High- and low-altitude populations were exposed experimentally to a range of oxygen and temperature conditions, simulating altitudinal conditions, and the transcriptomic responses explored. SNP densities are assessed to identify signatures of selective pressure and their link to differentially expressed genes. The high-altitude A. caliginosa population had lower differential expression and fewer co-expressed genes between conditions, indicating a more condition-refined epigenetic response. Genes identified as under adaptive pressure through Fst and nucleotide diversity in the high-altitude population clustered around the differentially expressed an upstream environmental response control gene, HMGB1. The high-altitude population of A. caliginosa indicated adaption and acclimatisation to high-altitude conditions and suggested resilience to extreme weather events. This mechanistic understanding could help offer a strategy in further identifying other species capable of maintaining soil fertility in extreme environments

Genome sequence of an Australian kangaroo, Macropus eugenii, provides insight into the evolution of mammalian reproduction and development.

BACKGROUND: We present the genome sequence of the tammar wallaby, Macropus eugenii, which is a member of the kangaroo family and the first representative of the iconic hopping mammals that symbolize Australia to be sequenced. The tammar has many unusual biological characteristics, including the longest period of embryonic diapause of any mammal, extremely synchronized seasonal breeding and prolonged and sophisticated lactation within a well-defined pouch. Like other marsupials, it gives birth to highly altricial young, and has a small number of very large chromosomes, making it a valuable model for genomics, reproduction and development. RESULTS: The genome has been sequenced to 2 × coverage using Sanger sequencing, enhanced with additional next generation sequencing and the integration of extensive physical and linkage maps to build the genome assembly. We also sequenced the tammar transcriptome across many tissues and developmental time points. Our analyses of these data shed light on mammalian reproduction, development and genome evolution: there is innovation in reproductive and lactational genes, rapid evolution of germ cell genes, and incomplete, locus-specific X inactivation. We also observe novel retrotransposons and a highly rearranged major histocompatibility complex, with many class I genes located outside the complex. Novel microRNAs in the tammar HOX clusters uncover new potential mammalian HOX regulatory elements. CONCLUSIONS: Analyses of these resources enhance our understanding of marsupial gene evolution, identify marsupial-specific conserved non-coding elements and critical genes across a range of biological systems, including reproduction, development and immunity, and provide new insight into marsupial and mammalian biology and genome evolution