<em>Kelussiaodoratissima</em> Mozaff.as a rich source of essential fatty acids andphthalides

Abstract Introduction: The present study is the first assessment of the fatty acids of leaf and essential oil compositions of new three habitats of aerial parts of K. odoratissima. Methods: The aerial parts of K. odoratissima from the three habitats were dried. The essential oils were obtained by hydrodistillation for 3 h in a Clevenger-type apparatus, then the analysis of the components was carried out using gas chromatography&ndash;mass spectrometry. To study the oil yield and fatty acids, the dried leaves subjected to extraction in hexane by using Soxhlet Apparatus. To analyze fatty acids from the oil fractions by gas chromatography technique, the oil was subjected to transesterification to obtain the fatty acid methyl esters, which, were dissolved in hexane and subjected to GC analysis. Results: According to the results, a total of 43 components, the major constitutes of essential oil compositions were (Z)-Ligustilide (76.45), Unknown-A (4.47), (E)-Ligustilide (2.57), (Z)-Butylidene phthalide (2.37), 5-pentyl cyclohexa-1,3-diene (1.57) and Kessane (0.77) in K. odoratissima. The sixteen fatty acids were separated &nbsp;from the oil (5 yield per 100 g dry matter). Linoleic acid (25.46), &alpha;-Linolenic acid (16.66), Palmitic acid (11.92), Oleic acid (9.33), Stearic acid (4.72), Petroselinic acid (2.53), Arachidonic acid (2.51) and Erucic acid (1.76) were major fatty acids. Conclusion: Generally, K. odoratissima is a rich source of essential fatty acids and phthalide derivatives, specially (Z)-ligustilide. This study was presented valuable information about the phytochemical properties, which can be useful for the future researches on the pharmacological effects of K. odoratissima

Differential responses of two fenugreek (Trigonella foenum-graecum L.) landraces pretreated with melatonin to prolonged drought stress and subsequent recovery

Abstract Background Drought impairs growth, disturbs photosynthesis, and induces senescence in plants, which results in crop productivity reduction and ultimately jeopardizes human food security. The objective of this study was to determine major parameters associated with drought tolerance and recovery ability of fenugreek (Trigonella foenum-graecum L.), by examining differential biochemical and phenological responses and underlying enzyme activities as well as melatonin roles during drought stress and re-watering for two contrasting landraces. Moreover, the relative expression of three key genes involved in the biosynthesis pathway of diosgenin, including SQS, CAS, and BG, was investigated. Results Depending on the conditions, drought stress enhanced the activity of antioxidant enzymes and the osmoregulating compounds, non-enzymatic antioxidants, hydrogen peroxide content, and lipid peroxidation levels in most cases. Severe drought stress accelerated flowering time in Shushtar landrace (SHR) but had no significant effects on Varamin (VR). Pretreatment with melatonin delayed flowering time in SHR and caused high drought resistance in this landrace. Furthermore, melatonin significantly enhanced drought adaptability in VR by improving plant recovery ability. Discussion Based on our results plants’ responses to drought stress and melatonin pretreatment were completely landrace-specific. Drought stress caused an increase in the relative expression of CAS gene and ultimately the accumulation of steroidal saponins in SHR. Melatonin compensated for the decrease in biomass production due to drought stress and finally increased steroidal saponins performance in SHR. Our study showed that melatonin can improve drought stress and recovery in fenugreek, but different factors such as genotype, melatonin concentration, and plant age should be considered

Kelussia odoratissima Mozaff. as a rich source of essential fatty acids and phthalides

Introduction: The present study is the first assessment of the fatty acids of leaf and essential oil compositions of new three habitats of aerial parts of K. odoratissima. Methods: The aerial parts of K. odoratissima from the three habitats were dried. The essential oils were obtained by hydrodistillation for 3 h in a Clevenger-type apparatus, then the analysis of the components was carried out using gas chromatography–mass spectrometry. To study the oil yield and fatty acids, the dried leaves subjected to extraction in hexane by using Soxhlet Apparatus. To analyze fatty acids from the oil fractions by gas chromatography technique, the oil was subjected to transesterification to obtain the fatty acid methyl esters, which, were dissolved in hexane and subjected to GC analysis. Results: According to the results, a total of 43 components, the major constitutes of essential oil compositions were (Z)-Ligustilide (76.45%), Unknown-A (4.47%), (E)-Ligustilide (2.57%), (Z)-Butylidene phthalide (2.37%), 5-pentyl cyclohexa-1,3-diene (1.57%) and Kessane (0.77%) in K. odoratissima. The sixteen fatty acids were separated from the oil (5% yield per 100 g dry matter). Linoleic acid (25.46%), α-Linolenic acid (16.66%), Palmitic acid (11.92%), Oleic acid (9.33%), Stearic acid (4.72%), Petroselinic acid (2.53%), Arachidonic acid (2.51%) and Erucic acid (1.76%) were major fatty acids. Conclusion: Generally, K. odoratissima is a rich source of essential fatty acids and phthalide derivatives, specially (Z)-ligustilide. This study was presented valuable information about the phytochemical properties, which can be useful for the future researches on the pharmacological effects of K. odoratissima

Artificial neural network genetic algorithm as powerful tool to predict and optimize in vitro proliferation mineral medium for G&#215;N15 rootstock

AbstractOne of the major obstacles to the micropropagation of Prunus rootstocks has, up until now, been the lack of a suitable tissue culture medium. Therefore, reformulation of culture media or modification of the mineral content might be a breakthrough to improve in vitro multiplication of G×N15 (garnem). We found artificial neural network in combination of genetic algorithm (ANN-GA) as a very precise and powerful modeling system for optimizing the culture medium, So that modeling the effects of MS mineral salts (NH4+, NO3-, PO42-, Ca2+, K+, SO42-, Mg2+ and Cl-) on in vitro multiplication parameters (the number of microshoots per explant, average length of microshoots, weight of calluses derived from the base of stem explants, and quality index of plantlets) of G×N15. showed high R2 correlation values of 87, 91, 87 and 74 between observed and predicted values were found for these four growth parameters, respectively.. According to the ANN-GA results, among the input variables, NH4+ and NO3- had the highest values of VSR in data set for the parameters studied. The ANN-GA showed that the best proliferation rate was obtained from medium containing (mM) 27.5 NO3-, 14 NH4+, 5 Ca2+, 25.9 K+, 0.7 Mg2+, 1.1 PO42-, 4.7 SO42- and 0.96 Cl-. The performance of the medium optimized by ANN-GA, denoted as YAS (Yadollahi, Arab and Shojaeiyan), was compared to that of standard growth media for all Prunus rootstock, including the Murashige and Skoog (MS) medium, (specific media) EM, Quoirin and Lepoivre (QL) medium and woody plant medium (WPM) Prunus. With respect to shoot length, shoot number per cultured explant and productivity (number of microshoots × length of microshoots), YAS was found to be superior to other media for in vitro multiplication of G×N15 rootstocks. In addition, our results indicated that by using ANN-GA, we were able to determine a suitable culture medium formulation to achieve the best in vitro productivity

The taxonomic significance of ddRADseq based microsatellite markers in the closely related species of Heracleum (Apiaceae)

Many studies on Heracleum have shown poor correspondence between observed molecular clusters and established taxonomic classification amongst closely related species. This might reflect both unresolved taxonomy but perhaps also a lack of good genetic markers. This lack of appropriate and cost effective species-specific genetic markers hinders a resolved relationship for the species complex, and this in turn causes profound management challenges for a genus that contains both endemic species, with important ecological roles, and species with an invasive potential. Microsatellites are traditionally considered markers of choice for comprehensive, yet inexpensive, analyses of genetic variation, including examination of population structure, species identity, linkage map construction and cryptic speciation. In this study, we have used double digest restriction site associated DNA sequencing (ddRADseq) to develop microsatellite markers in Heracleum rechingeri. Genomic DNA from three individuals were digested with Sbf1 and Nde1 and size selected for library construction. The size-selected fragments were sequenced on an Ion Torrent sequencer and a total of 54 microsatellite sequences were bioinformatically confirmed. Twenty five loci were then tested for amplification, resulting in 19 of these being successfully amplified across eight species, comprising both the so-called thick-stemmed species (H. persicum, H. rechingeri, H. gorganicum and H. lasiopetalum), and thin-stemmed species (H. anisactis, H. pastinasifolium and H. transcaucasicum). Both Bayesian and distance-based clustering, and principal coordinate analyses clearly separated these into two groups. Surprisingly, three H. pastinacifolium populations were not separated from populations of the morphologically similar endemic species, H. anisactis, suggesting lack of genetic differentiation. Likewise, high genetic similarity was found between H. persicum and H. rechingeri populations, questioning taxonomic separation at the species level between these taxa. Further analyses are needed to re-evaluate the taxonomic significance of observed morphological variability currently applied to distinguish these sister taxa. Nevertheless, our results represent progress in the effort to develop cost-efficient molecular tools for species discrimination in this genus.publishedVersio

Evaluation of Some Postharvest Storage Approaches on Essential Oil Characteristics of Fresh Organic Damask Rose (Rosa damascena Mill.) Flowers

Damask rose (Rosa damascena Mill.) is an economically important species in the Rosaceae family for rose oil and rose water production, obtained exclusively from freshly-gathered Damask rose flowers. Iran is famous for producing organic rose essential oil, mainly used in the perfume industry and for folk medicines due to its healing properties. Due to their high perishability, postharvest handling of the flowers prior to oil extraction are a key point in rose essential oil production. In this study, postharvest handling conditions of Damask rose flowers were evaluated for the effect on yield and quality of the extracted essential oils. Organically-grown flowers were stored under different conditions included packaging in polyethylene bags and immersing into water containers, and then held at 25 ± 2 °C or 4 ± 1 °C for 1, 2, or 3 days. Identification of the chemical composition of the essential oils was performed by GC and GC/MS. The results indicated that petal storage in water resulted in the highest essential oil content and quality, and storage in polyethylene bags resulted in the greatest loss, compared to those from unstored petals. The results provide a reference for interested groups such as producers and consumers who are concerned about Damask rose flower storage and subsequent oil extraction

Genome size : A novel predictor of nut weight and nut size of walnut trees

Plant genetic diversity is the fundamental of plant-breeding programs to improve desirable characteristics. Hence, evaluation of genetic diversity is the first step in fruit-breeding programs. Accordingly, the current study was carried out to evaluate 25 superior walnut genotypes in respect of phenotypic and cytological characteristics. For this purpose, 560 walnut genotypes in southwest of Iran were evaluated based on UPOV and International Plant Genetic Resources Institute (IPGRI) descriptor. After a 2-year primary evaluation, 25 superior genotypes were selected for future phenotypic and genome size assessment. Flow cytometry was used to estimate genome size of the selected superior genotypes. A high genetic diversity was found in walnut population collected from the southwest of Iran. The selected superior genotypes had high yield, lateral bearing, thin-shell thickness (0.90–1.64 mm), high nut (12.54–19.80 g) and kernel (7.02– 9.91 g) weight with light (L) to extra light (EL) kernel color which easily can be removed from the shell. Also, FaBaCh2 genotype turned out to be protogynous being important as a pollinizer cultivar. In addition to extensive phenotypic analysis, genome size was determined. The studied genotypes were diploid (2n = 2x = 32) and varied in genome size from 1.29 (FaBaAv2) to 1.40 pg (FaBaNs12). Correlation analysis showed that lateral bearing, budbreak date, nut size, and weight were the main variables contributing to walnut production. A linear relationship was found between genome size and nut weight (r = 0.527**), kernel weight (r = 0.551**), and nut size index (NSI) (r = 0.487**). Therefore, genome size can be considered as a strong and valuable tool to predict nut and kernel weight and nut size

Effects of nutrient media, different cytokinin types and their concentrations on in vitro multiplication of G × N15 (hybrid of almond × peach) vegetative rootstock

The objective of this research was to assess the effects of different media i.e. Murashige and Skoog (MS) and Quoirin and Lepoivre (QL), cytokinin type i.e. 6-Benzyladenin (BA) and 6-Benzylaminopurine (BAP) and cytokinin concentration on in vitro proliferation of the G × N15 rootstock. To evaluate the effects of different media and cytokinin type, two separate experiments were conducted as factorial based on completely randomized design, and single nodes were used as explants. The results showed that MS nutrient medium was found to be superior to QL nutrient medium. Regarding the interaction between media and growth regulators, the best interaction was found in MS medium supplemented with 1 mg l−1 BAP resulting in 8.5 new micro shoots/explant while 7.75 shoots were observed in MS medium containing 1.25 mg l−1 BA. The longest length of new micro-shoots (2.10 cm) was obtained in hormone-free MS medium. Findings of this study showed that there is a significant correlation between the hormone level and plantlet height and formed callus weight so that an increase in BAP and BA levels in both of MS and QL media resulted significantly in height decrease and callus weight increase. The results also suggest that the best and the worst plantlets in terms of quality were observed in hormone-free QL medium and MS medium supplemented with 1.25 mg l−1, respectively. These results reflect the fact that the presence of high amounts of NH4NO3 and cytokinin especially BAP in culture medium triggered inhibitory effect on shoot growth

Genomic characterization of self-incompatibility ribonucleases in the Central Asian pear germplasm and introgression of new alleles from other species of the genus Pyrus

The Pyrus species exhibit the so-called S-RNase based gametophytic self-incompatibility system, which is considered to be the most widespread self-incompatibility system among flowering plants. In this study, 57 Iranian pear (Pyrus communis L.) domestic cultivars and wild genotypes, plus 21 European pear cultivars used as references, were genotyped adopting a PCR-based genotyping assay using consensus and allele-specific primers. The results revealed traces of significant genetic contribution in the Iranian traditional varieties and genotypes from other Pyrus species; the genetic contribution of Japanese pear clearly emerged with the detection of some Pyrus pyrifolia S-RNase alleles. Moreover, our results highlighted the presence of three new S-RNase alleles (named S126, S127, and S128) that were not previously identified in P. communis, possibly introduced in the germplasm of cultivated pear through gene transfer from other cultivated or wild species