8 research outputs found
AFLP-analysis of genetic diversity in soybean [Glycine max (l.) Merr.] cultivars Russian and foreign selection
ArticleGenetic diversity of 11 cultivars of soybean (Glycine max) from a VIR (N.I. Vavilov
Institute of Plant Genetic Resources) collection was analyzed by the AFLP (amplified fragment
length polymorphism) technique. From 18 tested primer combinations, both of them were
selected for further analysis. From these two primer combinations (E32/M60 and E32/M59),
90 DNA fragments were obtained, 39 (43%) of them were polymorphic. Unique DNA fragments
were found in accessions SibNIISHoz 6, Soer-4, Determinant. The Jaccard's similarity indices
varied from 0.79 (between Sonata and Ugra) to 0.94 (between Ugra and Garmoniya) with an
average of 0.775. Cluster analysis placed the cultivars into the several groups but separation in
groups was not related to their origin or biological characteristics, even though cultivars Nordik
and Soer-4 (cultivars recommended for Central Black Earth region of Russia) clustered together
on the dendrogram. The obtained high values of Jaccard genetic similarity index and low level of
polymorphism and differentiation illustrated a relatively low genetic diversity in our studied
cultivars, which correlated with different other studies on soybean genetic diversity with
AFLP-analysis
ΠΠΏΡΠΈΠΌΠΈΠ·Π°ΡΠΈΡ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΠΎΠ² ΡΡΠΌΠΎΠΏΠΎΠ΄Π°Π²Π»Π΅Π½ΠΈΡ ΡΠΈΠ»ΡΡΡΠΎΠ² Π²ΡΡΠΎΠΊΠΎΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΠΈΡΠ΅Π»ΡΠ½ΡΡ ΠΈΡΡΠΎΡΠ½ΠΈΠΊΠΎΠ² ΠΏΠΈΡΠ°Π½ΠΈΡ
For a noise-reduction filter, the parameters of the manufactured device often do not fit the expected parameters during development, what is caused by the spread of the parameters of the electronic components or their parasitic parameters, as well as due to the presence of its own capacitance and inductance of the frequency response assembly. In this article, the influence of parasitic parameters of components and fasteners on the filter frequency response is considered. Utilizing the electronic simulator software, the filter parameters were modeled in the frequency range from 10 kHz to 1 GHz. Based on the simulation results, recommendations were given for optimizing the filter mounting elements and changing the inductance of low-frequency chokes.ΠΠ°ΡΠ°ΠΌΠ΅ΡΡΡ Π³ΠΎΡΠΎΠ²ΠΎΠ³ΠΎ ΡΠ»Π΅ΠΊΡΡΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΈΠ·Π΄Π΅Π»ΠΈΡ ΠΌΠΎΠ³ΡΡ Π½Π΅ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΠΎΠ²Π°ΡΡ ΡΠ°ΡΡΠ΅ΡΠ½ΡΠΌ, ΡΡΠΎ ΠΎΠ±ΡΡΠ»ΠΎΠ²Π»Π΅Π½ΠΎ, Π½Π°ΠΏΡΠΈΠΌΠ΅Ρ, Π½Π΅ΡΡΡΠ΅Π½Π½ΡΠΌΠΈ ΠΏΠ°ΡΠ°Π·ΠΈΡΠ½ΡΠΌΠΈ ΡΠ²ΡΠ·ΡΠΌΠΈ. ΠΠΎ ΡΡΠΎΠΉ ΠΏΡΠΈΡΠΈΠ½Π΅ ΠΏΡΠΈ ΠΊΠΎΠ½ΡΡΡΡΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ ΠΏΠΎΠΌΠ΅Ρ
ΠΎΠΏΠΎΠ΄Π°Π²Π»ΡΡΡΠΈΡ
ΡΠΈΠ»ΡΡΡΠΎΠ² ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΡΠΈΠ»ΡΡΡΠ°ΡΠΈΠΈ ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ Π·Π°Π½ΠΈΠΆΠ΅Π½Π° ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ ΠΎΠΆΠΈΠ΄Π°Π΅ΠΌΠΎΠΉ. ΠΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ ΠΌΠΎΠ΄Π΅Π»ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ Π°ΠΌΠΏΠ»ΠΈΡΡΠ΄Π½ΠΎ-ΡΠ°ΡΡΠΎΡΠ½ΠΎΠΉ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠΈ ΡΠΈΠ»ΡΡΡΠ°, ΠΎΠ±ΡΡΠ»ΠΎΠ²Π»Π΅Π½Π½ΠΎΠ΅ Π²Π»ΠΈΡΠ½ΠΈΠ΅ΠΌ ΠΏΠ°ΡΠ°Π·ΠΈΡΠ½ΡΡ
ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΠΎΠ² ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠΎΠ² ΠΈ ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ² ΠΌΠ΅Ρ
Π°Π½ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΊΡΠ΅ΠΏΠ΅ΠΆΠ°, Π² Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Π΅ ΡΠ°ΡΡΠΎΡ ΠΎΡ 10 ΠΊΠΡ Π΄ΠΎ 1 ΠΠΡ. ΠΡΠ΅Π΄Π»ΠΎΠΆΠ΅Π½Ρ ΡΠ΅ΠΊΠΎΠΌΠ΅Π½Π΄Π°ΡΠΈΠΈ ΠΏΠΎ ΠΌΠΎΠ΄ΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ² ΠΌΠ΅Ρ
Π°Π½ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΊΡΠ΅ΠΏΠ»Π΅Π½ΠΈΡ ΠΈ ΠΎΠΏΡΠΈΠΌΠΈΠ·Π°ΡΠΈΠΈ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΠΎΠ² Π΄ΡΠΎΡΡΠ΅Π»Π΅ΠΉ Π½ΠΈΠ·ΠΊΠΎΠΉ ΡΠ°ΡΡΠΎΡΡ
Impaired complex I repair causes recessive Leber's hereditary optic neuropathy
Leber's hereditary optic neuropathy (LHON) is the most frequent mitochondrial disease and was the first to be genetically defined by a point mutation in mitochondrial DNA (mtDNA). A molecular diagnosis is achieved in up to 95% of cases, the vast majority of which are accounted for by 3 mutations within mitochondrial complex I subunit-encoding genes in the mtDNA (mtLHON). Here, we resolve the enigma of LHON in the absence of pathogenic mtDNA mutations. We describe biallelic mutations in a nuclear encoded gene, DNAJC30, in 33 unsolved patients from 29 families and establish an autosomal recessive mode of inheritance for LHON (arLHON), which to date has been a prime example of a maternally inherited disorder. Remarkably, all hallmarks of mtLHON were recapitulated, including incomplete penetrance, male predominance, and significant idebenone responsivity. Moreover, by tracking protein turnover in patient-derived cell lines and a DNAJC30-knockout cellular model, we measured reduced turnover of specific complex I N-module subunits and a resultant impairment of complex I function. These results demonstrate that DNAJC30 is a chaperone protein needed for the efficient exchange of complex I subunits exposed to reactive oxygen species and integral to a mitochondrial complex I repair mechanism, thereby providing the first example to our knowledge of a disease resulting from impaired exchange of assembled respiratory chain subunits
Selection combinations of primers for determining intervarietal and intravarietal polymorphism Glycine hispida
The article describes the experience of selecting combinations of primers for AFLP-analysis intravarietal and intervarietal polymorphism soybean accessions. During the experiment, has been tested 18 primer combinations. As a result, were selected 5 primer combinations, the most informative labeling for the soybean genome. Total detect 132 AFLP-fragments, of which 60 were polymorphic and characterized by individual genotypes varieties
AFLP-analysis of genetic diversity in soybean [Glycine max (L.) Merr.] cultivars Russian and foreign selection
Genetic diversity of 11 cultivars of soybean (Glycine max) from a VIR (N.I. Vavilov Institute of Plant Genetic Resources) collection was analyzed by the AFLP (amplified fragment length polymorphism) technique. From 18 tested primer combinations, both of them were selected for further analysis. From these two primer combinations (E32/M60 and E32/M59), 90 DNA fragments were obtained, 39 (43%) of them were polymorphic. Unique DNA fragments were found in accessions SibNIISHoz 6, Soer-4, Determinant. The Jaccard's similarity indices varied from 0.79 (between Sonata and Ugra) to 0.94 (between Ugra and Garmoniya) with an average of 0.775. Cluster analysis placed the cultivars into the several groups but separation in groups was not related to their origin or biological characteristics, even though cultivars Nordik and Soer-4 (cultivars recommended for Central Black Earth region of Russia) clustered together on the dendrogram. The obtained high values of Jaccard genetic similarity index and low level of polymorphism and differentiation illustrated a relatively low genetic diversity in our studied cultivars, which correlated with different other studies on soybean genetic diversity with AFLP-analysis. Β© 2017, Eesti Pollumajandusulikool. All rights reserved
AFLP-analysis of genetic diversity in soybean [Glycine max (L.) Merr.] cultivars Russian and foreign selection
Genetic diversity of 11 cultivars of soybean (Glycine max) from a VIR (N.I. Vavilov Institute of Plant Genetic Resources) collection was analyzed by the AFLP (amplified fragment length polymorphism) technique. From 18 tested primer combinations, both of them were selected for further analysis. From these two primer combinations (E32/M60 and E32/M59), 90 DNA fragments were obtained, 39 (43%) of them were polymorphic. Unique DNA fragments were found in accessions SibNIISHoz 6, Soer-4, Determinant. The Jaccard's similarity indices varied from 0.79 (between Sonata and Ugra) to 0.94 (between Ugra and Garmoniya) with an average of 0.775. Cluster analysis placed the cultivars into the several groups but separation in groups was not related to their origin or biological characteristics, even though cultivars Nordik and Soer-4 (cultivars recommended for Central Black Earth region of Russia) clustered together on the dendrogram. The obtained high values of Jaccard genetic similarity index and low level of polymorphism and differentiation illustrated a relatively low genetic diversity in our studied cultivars, which correlated with different other studies on soybean genetic diversity with AFLP-analysis. Β© 2017, Eesti Pollumajandusulikool. All rights reserved
FLOW-CYTOFLUORIMETRIC ANALYSIS OF CYTOTOXICITY OF YERSINIA PESTIS STRAINS
Aim. Study cytotoxic effect of Yersinia pestis strains with various degree of virulence on leukocytes of whole defibrinated human blood. Materials and methods. 15 strains of Y. pestis were used in the study. DNA histograms were registered using flow cytofluorimeter. Results. Possibility of using cytotoxicity to differentiate Y. pestis into virulent and avirulent wfas experimentally proven. The data obtained was comparable with LD5o for mice and did not contradict molecular-genetic characteristics of the strains. Conclusion. The approach proposed allows to obtain reliable results and is perspective for evaluation of virulence for Y. pestis strains
DETERMINATION OF VIRULENCE PROPERTIES OF PATHOGENIC MICROORGANISMS IN VITRO: STATE-OF-ART
Various methods for evaluation of virulence properties of causative agents of infectious diseases in vitro were analyzed: molecular-genetic, cultural-biochemical, immunologic, physiologic. Predominant use of molecular-genetic methods, expediency of a complex approach, relevance of search of novel informative parameters of virulence are noted. Study of biological properties of pathogens in vitro is the first screening stage of evaluation of their virulence