A comparative study of Tam3 and Ac transposition in transgenic tobacco and petunia plants

Transposition of the Anthirrinum majus Tam3 element and the Zea mays Ac element has been monitored in petunia and tobacco plants. Plant vectors were constructed with the transposable elements cloned into the leader sequence of a marker gene. Agrobacterium tumefaciens-mediated leaf disc transformation was used to introduce the transposable element constructs into plant cells. In transgenic plants, excision of the transposable element restores gene expression and results in a clearly distinguishable phenotype. Based on restored expression of the hygromycin phosphotransferase II (HPTII) gene, we established that Tam3 excises in 30% of the transformed petunia plants and in 60% of the transformed tobacco plants. Ac excises from the HPTII gene with comparable frequencies (30%) in both plant species. When the β-glucuronidase (GUS) gene was used to detect transposition of Tam3, a significantly lower excision frequency (13%) was found in both plant species. It could be shown that deletion of parts of the transposable elements Tam3 and Ac, removing either one of the terminal inverted repeats (TIR) or part of the presumptive transposase coding region, abolished the excision from the marker genes. This demonstrates that excision of the transposable element Tam3 in heterologous plant species, as documented for the autonomous element Ac, also depends on both properties. Southern blot hybridization shows the expected excision pattern and the reintegration of Tam3 and Ac elements into the genome of tobacco plants.

Emerging roles of ATF2 and the dynamic AP1 network in cancer

Cooperation among transcription factors is central for their ability to execute specific transcriptional programmes. The AP1 complex exemplifies a network of transcription factors that function in unison under normal circumstances and during the course of tumour development and progression. This Perspective summarizes our current understanding of the changes in members of the AP1 complex and the role of ATF2 as part of this complex in tumorigenesis.Fil: Lopez Bergami, Pablo Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina; ArgentinaFil: Lau, Eric . Burnham Institute for Medical Research; Estados UnidosFil: Ronai, Zeev . Burnham Institute for Medical Research; Estados Unido

Violence Detection using Embedded GPU

Since the CCTV cameras been introduced in this world, society has started to depend heavily on the usage of this technology for the high security purposes in most of the public and private areas. It is convenient to use these CCTV footages in courts as evidence and has been beneficial many times. But these footages are given priority and checked later when the incident has already taken place and that too after some period of time and not in real-time of happening. The screening of the multiple CCTV footages on a single monitor is done with very less efficiency as the ratio of number of CCTV footages to that of number of surveillance staff is very high. Also, the human unreliable supervision due to many reasons like tiredness from physical or mental effort, worker boredom, or discontinuous observation make the surveillance more inefficient. To address the issue and automatically detect the violent scenes using surveillance cameras and Embedded GPU in real-time we have developed this project for the benefit of our society. As the alert is generated in real-time, the security can take action immediately to prevent any further damage or mishappening in the crowd. Our primary objective is to automatically differentiate between violent activities and non-violent activities through CCTV surveillance cameras and automatically display the security alert on the screen as soon as any violent activity is captured and thus ensuring the safety of our society