Baseline marine benthic surveys in the Maltese Islands (Central Mediterranean)

Extract from: MEDCOAST 97 : Proceedings of the Third International Conference on the Mediterranean Coastal Environment, Qawra, Malta / E. Ozhan (editor), (1997)During the period 1991 to 1997, extensive baseline studies of the submarine geomorphology, infralittoral habitats, macrobenthic assemblages, and demersal fish fauna, were carried out in 14 different sites around the Maltese Islands, using conventional SCUBA diving techniques. These were made as part of assessments of the environmental impact of existing or proposed projects, or in order to produce an inventory of coastal resources. In total, these surveys covered a sea-bed area of ca. 7.55Km² and a coastline length of ca 24 km, and represent the most extensive biological surveys of the marine environment carried out to date in the Maltese Islands. The results of these surveys have been presented as maps showing the type, location, and spatial extent of these physical and biological features. The Peres & Picard (1964) scheme, as adapted by Pen~s (1967; 1982) was used to classify and characterize the benthic assemblages recorded during the surveys. Our results show that, in tenus of spatial extent, the most important macrobenthic assemblages are the communities of photophilic 'algae on hard substrata, meadows of the sea-grass Posidonia oceanica and communities of bare well-sorted sand. For all these, many subtypes and facies exist, depending on the light intensity, hydrodynamic conditions, microtopography, sediment granulometry and other edaphic factors, and anthropic influences, including pollution. Other assemblages with a limited spatial distribution include meadows of the sea-grass (vmodocea nodosa and of the Lessepsian immigrant Halophila stipu/acea on sandy bottoms, the assemblages of boulder fields which are complexes of photophilic and sciaphilic communities, and those of marine caves. Our maps are supplemented by descriptions of the different habitats and macrobenthjc assemblages, species lists, and semi-quantitative data on percentage cover for the dominant macroalgae, shoot density counts for sea-grass meadows, and population density counts for the main macrofaunal species. These maps and data-sets are intended to be used (i) for assessing the status of habitats, species assemblages, and individual species, around the Maltese Islands in order to recommend appropriate measures for their conservation, including inclusion in red data lists; (ii) for designation of marine protected areas; and (iii) as baselines against which future monitoring studies and surveys can be compared.peer-reviewe

The macrobenthic species of the infralittoral to circalittoral transition zone off the northeastern coast of Malta (Central Mediterranean)

The macrobenthic assemblages of the sedimentary bottoms off the northeastern coast of Malta were studied during the summer of 1993. Crab and dredge samples were collected from depths of 21-75tn from an area of approximately 48 km2 off Comino, Mellieha Bay and St. Paul's Bay. Below ctr. 40m the bottorn was predominantly sandy with meadows of Posidonia oceanica, giving way to bare sand it1 the lower infralittoral. Between 45rn and 75r11, the bottom consisted of organogenic gravel with abundunt free-living culcureous rhodophytes, predominantly the corallines Phymatolithon calcareum and Lithothamnion corallioides. Such bot~oms, known as maerl, have not been reported previously from the Maltese Islands. 19 species of macroalgae, I phanerogam, and 108 species of macrofaunu were recorded, the bulk of these from the maerl bottom. An additional 122 species of molluscs occurred as etnpty shells. Maerl bottoms support a high species richness, even if abundance is low. There is evidence to suggest that maerl is the predominant bottom type all along the northeastern coast of the Maltese Islands in the tramition between the lower infralittoral and the circalittoral, which occurs at depths of 45-75m.peer-reviewe

Dimethyl fumarate in patients admitted to hospital with COVID-19 (RECOVERY): a randomised, controlled, open-label, platform trial

Dimethyl fumarate (DMF) inhibits inflammasome-mediated inflammation and has been proposed as a treatment for patients hospitalised with COVID-19. This randomised, controlled, open-label platform trial (Randomised Evaluation of COVID-19 Therapy [RECOVERY]), is assessing multiple treatments in patients hospitalised for COVID-19 (NCT04381936, ISRCTN50189673). In this assessment of DMF performed at 27 UK hospitals, adults were randomly allocated (1:1) to either usual standard of care alone or usual standard of care plus DMF. The primary outcome was clinical status on day 5 measured on a seven-point ordinal scale. Secondary outcomes were time to sustained improvement in clinical status, time to discharge, day 5 peripheral blood oxygenation, day 5 C-reactive protein, and improvement in day 10 clinical status. Between 2 March 2021 and 18 November 2021, 713 patients were enroled in the DMF evaluation, of whom 356 were randomly allocated to receive usual care plus DMF, and 357 to usual care alone. 95% of patients received corticosteroids as part of routine care. There was no evidence of a beneficial effect of DMF on clinical status at day 5 (common odds ratio of unfavourable outcome 1.12; 95% CI 0.86-1.47; p = 0.40). There was no significant effect of DMF on any secondary outcome

Dimethyl fumarate in patients admitted to hospital with COVID-19 (RECOVERY): a randomised, controlled, open-label, platform trial

Dimethyl fumarate (DMF) inhibits inflammasome-mediated inflammation and has been proposed as a treatment for patients hospitalised with COVID-19. This randomised, controlled, open-label platform trial (Randomised Evaluation of COVID-19 Therapy [RECOVERY]), is assessing multiple treatments in patients hospitalised for COVID-19 (NCT04381936, ISRCTN50189673). In this assessment of DMF performed at 27 UK hospitals, adults were randomly allocated (1:1) to either usual standard of care alone or usual standard of care plus DMF. The primary outcome was clinical status on day 5 measured on a seven-point ordinal scale. Secondary outcomes were time to sustained improvement in clinical status, time to discharge, day 5 peripheral blood oxygenation, day 5 C-reactive protein, and improvement in day 10 clinical status. Between 2 March 2021 and 18 November 2021, 713 patients were enroled in the DMF evaluation, of whom 356 were randomly allocated to receive usual care plus DMF, and 357 to usual care alone. 95% of patients received corticosteroids as part of routine care. There was no evidence of a beneficial effect of DMF on clinical status at day 5 (common odds ratio of unfavourable outcome 1.12; 95% CI 0.86-1.47; p = 0.40). There was no significant effect of DMF on any secondary outcome

Rain induces temporary shifts in epiphytic bacterial communities of cucumber and tomato fruit

Understanding weather-related drivers of crop plant-microbiome relationships is important for food security and food safety in the face of a changing climate. Cucumber and tomato are commercially important commodities that are susceptible to plant disease and have been implicated in foodborne disease outbreaks. To investigate the influence of precipitation on plant-associated microbiomes, epiphytically associated bacterial communities of cucumber and tomato samples were profiled by 16 S rRNA gene sequencing (V1-V3) in the days surrounding two rain events over a 17-day period. Following rain, α (within-sample) diversity measured on cucumber and tomato fruit surfaces, but not tomato leaf surfaces, increased significantly and remained elevated for several days. Bacterial β (between-sample) diversity on cucumber and tomato fruit responded to precipitation. In the cucumber fruit surface (carpoplane), notable shifts in the families Xanthomonadaceae, Oxalobacteriaceae, Sphingobacteriaceae and Comamonadaceae were detected following precipitation. In the tomato carpoplane, shifts were detected in the families Enterobacteriaceae and Xanthomonadaceae following the first rain event, and in the Pseudomonadaceae and Oxalobacteriaceae following the second rain event. Few taxonomic shifts were detected in the tomato leaf surface (phylloplane). Exploring rain-induced shifts in plant microbiomes is highly relevant to crop protection, food safety and agroecology, and can aid in devising ways to enhance crop resilience to stresses and climate fluctuations

Cultivar was more influential than bacterial strain and other experimental factors in recovery of Escherichia coli O157:H7 populations from inoculated live Romaine lettuce plants

ABSTRACTThe varied choice of bacterial strain, plant cultivar, and method used to inoculate, retrieve, and enumerate Escherichia coli O157:H7 from live plants could affect comparability among studies evaluating lettuce–enterobacterial interactions. Cultivar, bacterial strain, incubation time, leaf side inoculated, and sample processing method were assessed for their influence in recovering and quantifying E. coli O157:H7 from live Romaine lettuce. Cultivar exerted the strongest effect on E. coli O157:H7 counts, which held up even when cultivar was considered in interactions with other factors. Recovery from the popularly grown green Romaine “Rio Bravo” was higher than from the red variety “Outredgeous.” Other modulating variables were incubation time, strain, and leaf side inoculated. Sample processing method was not significant. Incubation for 24 hours post-lettuce inoculation yielded greater counts than 48 hours, but was affected by lettuce cultivar, bacterial strain, and leaf side inoculated. Higher counts obtained for strain EDL933 compared to a lettuce outbreak strain 2705C emphasized the importance of selecting relevant strains for the system being studied. Inoculating the abaxial side of leaves gave higher counts than adaxial surface inoculation, although this factor interacted with strain and incubation period. Our findings highlight the importance of studying interactions between appropriate bacterial strains and plant cultivars for more relevant research results, and of standardizing inoculation and incubation procedures. The strong effect of cultivar exerted on the E. coli O157:H7-lettuce association supports the need to start reporting cultivar information for illness outbreaks to facilitate the identification and study of plant traits that impact food safety risk.IMPORTANCEThe contamination of Romaine lettuce with Escherichia coli O157:H7 has been linked to multiple foodborne disease outbreaks, but variability in the methods used to evaluate E. coli O157:H7 association with live lettuce plants complicates the comparability of different studies. In this study, various experimental variables and sample processing methods for recovering and quantifying E. coli O157:H7 from live Romaine lettuce were assessed. Cultivar was found to exert the strongest influence on E. coli O157:H7 retrieval from lettuce. Other modulating factors were bacterial incubation time on plants, strain, and leaf side inoculated, while sample processing method had no impact. Our findings highlight the importance of selecting relevant cultivars and strains, and of standardizing inoculation and incubation procedures, in these types of assessments. Moreover, results support the need to start reporting cultivars implicated in foodborne illness outbreaks to facilitate the identification and study of plant traits that impact food safety risk

Soil Microclimate and Persistence of Foodborne Pathogens Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella enterica Newport in Soil Affected by Mulch Type

Mulching is a common agricultural practice that benefits crop production through soil moisture retention, weed suppression, and soil temperature regulation. However, little is known about the effect of mulch on foodborne pathogens present in soil. In this study, the influence of polyethylene plastic, biodegradable corn-based plastic, paper, and straw mulches on Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella enterica Newport populations in soil was investigated. Silt loam soil in troughs was inoculated with a cocktail of the pathogens and covered with mulch or left bare, then incubated for 21 days, during which bacteria were enumerated and environmental parameters monitored. Bacterial counts declined in all treatments over time (p < 0.001) but persisted at 21 days at 0.8–0.95 log CFU/g. Pathogens also declined as a factor of mulch cover (p < 0.01). An exponential decay with asymptote model fit to the data revealed slower rates of decline in soil under mulches for all pathogens (p < 0.05) relative to bare soil. Compared to the average for all treatments, rates of decay in bare soil were 0.60 (p < 0.001), 0.45 (p < 0.05), and 0.63 (p < 0.001) log CFU/g/d for E. coli O157:H7, L. monocytogenes, and Salmonella, respectively. Linear multiple regression revealed that soil hydrological parameters were positively correlated (p < 0.05) with bacterial counts, while day soil temperatures were negatively correlated (p < 0.001), suggesting that higher day temperatures and lower moisture content of bare soil contributed to the faster decline of pathogens compared to mulched soil. A microcosm experiment using field soil from lettuce cultivation suggested no influence of prior mulch treatment on pathogens. In summary, pathogen decline in soil was modified by the soil microclimate created under mulch covers, but the effect appeared was restricted to the time of soil cover. Slower decline rates of pathogens in mulched soil may pose a risk for contamination of fresh market produce crops