Genomic analysis of carboxyl/cholinesterase genes in the silkworm Bombyx mori

<p>Abstract</p> <p>Background</p> <p>Carboxyl/cholinesterases (CCEs) have pivotal roles in dietary detoxification, pheromone or hormone degradation and neurodevelopment. The recent completion of genome projects in various insect species has led to the identification of multiple CCEs with unknown functions. Here, we analyzed the phylogeny, expression and genomic distribution of 69 putative CCEs in the silkworm, <it>Bombyx </it><it>mori </it>(Lepidoptera: Bombycidae).</p> <p>Results</p> <p>A phylogenetic tree of CCEs in <it>B. mori </it>and other lepidopteran species was constructed. The expression pattern of each <it>B. mori </it>CCE was also investigated by a search of an expressed sequence tag (EST) database, and the relationship between phylogeny and expression was analyzed. A large number of <it>B. mori </it>CCEs were identified from a midgut EST library. CCEs expressed in the midgut formed a cluster in the phylogenetic tree that included not only <it>B. mori </it>genes but also those of other lepidopteran species. The silkworm, and possibly also other lepidopteran species, has a large number of CCEs, and this might be a consequence of the large cluster of midgut CCEs. Investigation of intron-exon organization in <it>B. mori </it>CCEs revealed that their positions and splicing site phases were strongly conserved. Several <it>B. mori </it>CCEs, including juvenile hormone esterase, not only showed clustering in the phylogenetic tree but were also closely located on silkworm chromosomes. We investigated the phylogeny and microsynteny of neuroligins in detail, among many CCEs. Interestingly, we found the evolution of this gene appeared not to be conserved between <it>B. mori </it>and other insect orders.</p> <p>Conclusions</p> <p>We analyzed 69 putative CCEs from <it>B. mori</it>. Comparison of these CCEs with other lepidopteran CCEs indicated that they had conserved expression and function in this insect order. The analyses showed that CCEs were unevenly distributed across the genome of <it>B. mori </it>and suggested that neuroligins may have a distinct evolutionary history from other insect order. It is possible that such an uneven genomic distribution and a unique neuroligin evolution are shared with other lepidopteran insects. Our genomic analysis has provided novel information on the CCEs of the silkworm, which will be of value to understanding the biology, physiology and evolution of insect CCEs.</p

Metodologia para estimação do mérito genético de animais com paternidade incerta sob inferência bayesiana.

Modelo animal reduzido com efeito materno; Modelo hierárquico bayesiano para paternidade incerta; Validação por simulação; Análise de dados de desempenho de um rebanho Hereford; Inferência Bayesiana; Análises do estudo de simulação; Análises de dados do rebanho Hereford; Critérios para escolha do melhor modelo; Inferência sobre dados simulados; Inferência nos dados do rebanho Hereford; Ganho de peso pós-desmama; Peso à desmama.bitstream/item/55802/1/BP32.pd

Estimativas de parâmetros genéticos para características de crescimento e reprodutivas para ovinos da raça Morada Nova.

Embora a raça Morada Nova possua grande importância para a região semiárida do Brasil, em virtude de sua resistência às adversidades climáticas, escassez de alimento e entre outras dificuldades, são poucos registros de estimativa de parâmetros genéticos para essa raça, sendo a maioria dos trabalhos com essa raça da década de 80. Objetivou-se com esse trabalho, estimar parâmetros genéticos para características de crescimento e reprodutivas de ovinos da raça Morada Nova, com a finalidade de dar subsídios ao delineamento do esquema de seleção do Núcleo de Melhoramento Genético Participativo da Embrapa Caprinos e Ovinos

Anionic Surfactant Organized Film Formation on a Viologen Thiol SAM-Modified Polycrystalline Au and Au(111) Electrodes

ナノダイナミクス国際シンポジウム 平成22年1月21日(木) 於長崎大学Nagasaki Symposium on Nano-Dynamics 2010 (NSND2010), January 21, 2010, Nagasaki University, Nagasaki, Japan, Invited Lectur

Perspective Chapter: Design and Characterization of Natural and Synthetic Soft Polymeric Materials with Biomimetic 3D Microarchitecture for Tissue Engineering and Medical Applications

Continuous work and developments in biomedical materials used in three-dimensional (3D) bioprinting have contributed to significant growth of 3D bioprinting applications in the production of personalized tissue-repairing membrane, skin graft, prostheses, medication delivery system, and 3D tissue engineering and regenerative medicine scaffolds. The design of clinic products and devices focus on new natural and synthetic biomedical materials employed for therapeutic applications in different 3D bioprinting technologies. Design and characterization of natural and synthetic soft polymeric materials with biomimetic 3D microarchitecture were considered. The natural soft polymeric materials would focus on new design bioinspired membranes containing supercritical fluids-decellularized dermal scaffolds for 3D bioprinting potential applications. Synthetic soft polymeric materials would focus on bioinspired polyvinyl alcohol (b-PVA) matrix with structural foam-wall microarchitectures. Characterization, thermal stability, and cell morphology of the b-PVA and the corresponding collagen-modified b-PVA were employed to evaluate their potential tissue engineering applications. Also, the b-PVA materials were conductive to HepG2 cells proliferation, migration, and expression, which might serve as a promising liver cell culture carrier to be used in the biological artificial liver reactor. TGA, DTG, DSC, SEM, and FTIR were employed to build up the effective system identification approach for biomimetic structure, stability, purity, and safety of target soft matrix

Identification and Expression Analysis of Ras Gene in Silkworm, Bombyx mori

Ras proteins play important roles in development especially for cell proliferation and differentiation in various organisms. However, their functions in the most insect species are still not clear. We identified three ras cDNAs from the silk worm, Bombyx mori. These sequences corresponded to three Ras of Drosophila melanogaster, but not to three mammalian Ras (H-Ras, K-Ras, N-Ras). Subsequently, the expression profiles of ras were investigated by quantitative real-time PCR using whole body of individuals from the embryonic to adult stages, and various tissues of 4th and 5th instar larvae. Each of three Bombyx ras showed different expression patterns. We also showed membrane localization of their products. These results indicate that the three Bombyx Ras are functional and have different roles

Melhoramento genético de base comunitária: técnicas de transferência de tecnologia para ovinos e caprinos.

Ao sistematizar essa experiência, buscou-se resgatar o processo de organização social dos produtores e a relação deles com os agentes de pesquisa no decorrer do período abordado, identificando-se nesse contexto as metodologias de TT que contribuíram para o alcance dos objetivos propostos pelo projeto. Com isso, espera-se oferecer a outros agentes de conhecimento - sejam eles instituições de pesquisa, empresas de extensão rural, criadores, produtores, técnicos, representantes do setor agropecuário ou gestores de políticas públicas - elementos importantes que sirvam de parâmetro para elaboração de estratégias de TT.bitstream/item/176720/1/CNPC-2017-se8.pd