ANALISIS KAPASITAS RUANG PARKIR DI PELABUHAN FERRY LABUAN BAJO

<p>Bivariate negative binomial generalized linear mixed model (NB-GLMM) for <i>Schistosoma mansoni</i> infection risk among schoolchildren Mbita, Kenya.</p

Both T-bet and GATA-3 were expressed in a single hepatic γ4 or γ13 cell.

<p>(A and B) Hepatic lymphocytes were isolated from <i>S. mansoni</i>-infected mice and the expressions of T-bet and GAT-3 were analyzed by flowcytometry after TCR stimulation at 6 weeks PI. The numbers in the upper, large insets represent percentages of each population divided by the expressions of T-bet and GATA-3 in γ4 (A) or γ13 (B) cells. The lower, small insets represent the data using isotype control antibodies. Similar results were obtained in three independent experiments.</p

<i>Schistosoma mansoni</i> infection induced robust increase of immune competent cells in the liver.

<p>(A) Hepatic cells isolated from 3 mice were pooled and the cell number was calculated. (B and C) Flowcytometric analysis was conducted with the liver lymphocytes prepared in (A) or the splenocytes. The percentages in (B) represent the proportions in CD3-positive population, and those in (C) express the proportions in population of (B). (A-C) each shows one representative result of three independent experiments.</p

DX5-negative as well as –positive cells displayed the unique cytokine production patterns.

<p>(A and B) Hepatic lymphocytes were isolated from <i>S. mansoni</i>-infected mice and flowcytometric analysis was conducted upon TCR ligation at 6 weeks PI. The numbers in the insets represent percentages of γ4 (A) or γ13 (B) cell population in CD4⁺ DX5-positive or -negative population. This experiment is representative of five independent experiments. (C) Data represent the mean + SD of five independent experiments.</p

CD4⁺ T cells doubly producing IFN-γ and IL-5 were rarely induced after <i>S. mansoni</i> infection.

<p>Hepatic lymphocytes were isolated from <i>S. mansoni</i>-infected mice at 6 weeks PI and ICS was conducted upon TCR ligation. (A) One representative result is shown. The numbers in the insets represent the percentages of γ4, γ13, or γ5 in CD4-positive population. (B) Data represent the mean values + SD of three independent experiments.</p

Hepatic αβ T cells are the responsible cells showing the unconventional cytokine profiles.

<p>Hepatic lymphocytes were isolated from <i>S. mansoni</i>-infected mice and flowcytometry was conducted after TCR ligation. (A) Expression levels of γδ TCR on CD4⁺ γ4 cells or γ13 cells were analyzed with the hepatic lymphocytes isoleted at 6 weeks PI. The values in the right insets indicate percentages of γδ TCR-positive or –negative population in CD4⁺ γ4 or γ13 cells. This experiment is a representative of four independent experiments. (B) The absolute numbers of γ4 cells (upper graph) or γ13 cells (lower graph) in αβ TCR- or γδ TCR-potitive population were investigated. Similar results were obtained in three independent experiments.</p

Additional file 1 of In vitro evaluation of the effect of galectins on Schistosoma mansoni motility

Supplementary Material