MRE11ヌクレアーゼは、DNA切断端の削り込み以後の過程にも機能し、相同組換えを促進する

付記する学位プログラム名: 充実した健康長寿社会を築く総合医療開発リーダー育成プログラム京都大学新制・課程博士博士(医学)甲第23091号医博第4718号新制||医||1050(附属図書館)京都大学大学院医学研究科医学専攻(主査)教授 篠原 隆司, 教授 増永 慎一郎, 教授 小川 誠司学位規則第4条第1項該当Doctor of Medical ScienceKyoto UniversityDFA

Method to determine argon metastable number density and plasma electron temperature from spectral emission originating from four 4p argon levels

A simple model and method is proposed here to determine argon metastable number densities and electron temperature with the assumption of a Maxwell-Boltzmann electron energy distribution. This method is based on the availability of experimental relative emission intensities of only four argon lines that originate from any of the 4p argon levels. The proposed model has a relatively wide range of validity for laboratory plasmas that contain argon gas and can be a valuable tool for the emerging field of atmospheric microplasmas, for which diagnostics is still limited

Pollen Grain Counting Using a Cell Counter

The number of pollen grains is a critical part of the reproductive strategies in plants and varies greatly between and within species. In agriculture, pollen viability is important for crop breeding. It is a laborious work to count pollen tubes using a counting chamber under a microscope. Here, we present a method of counting the number of pollen grains using a cell counter. In this method, the counting step is shortened to 3 min per flower, which, in our setting, is more than five times faster than the counting chamber method. This technique is applicable to species with a lower and higher number of pollen grains, as it can count particles in a wide range, from 0 to 20,000 particles, in one measurement. The cell counter also estimates the size of the particles together with the number. Because aborted pollen shows abnormal membrane characteristics and/or a distorted or smaller shape, a cell counter can quantify the number of normal and aborted pollen separately. We explain how to count the number of pollen grains and measure pollen size in Arabidopsis thaliana, Arabidopsis kamchatica, and wheat (Triticum aestivum)

Influence of Drying and Post-drying Conditions on the Head Rice Yield of Aromatic Rice

The effects of various drying temperatures, low final moisture, and post-drying duration, on fissure formation and head rice yield (HRY) reduction in aromatic short-grain rice, were investigated. The results of post-drying duration showed that most fissured kernels were produced within 12-h after drying process ceased and then became to stable within 24 h for all treatments. The whole kernel percentage of Kaori aromatic rice was reduced significantly (P < 0.01) when rice was dried at 50oC and 60oC for both standard and low final moisture content (FMC). For a given post-drying duration and conditions, low FMC treatments exhibited more HRY reduction than did standard FMC. It is well known that moisture gradient has much influence in producing fissure in kernels. In addition, increased difference between immediate storage temperature and drying air temperature augmented the increase in the percentage of fissured kernels (P < 0.01)

Oxidation of CuSn alloy nanotree and application for gas sensors

The CuSn alloy nanotree formed by DC electroplating is a true three-dimensional (3D) structure with many branches that separate the trunk perpendicularly. We carried out the oxidation of CuSn nanotrees in atmosphere in order to study the possibility of such nanotrees for application to sensors. It was confirmed that the oxygen concentration in the CuSn nanotree oxide increased with temperature and reached 40 at. % at 350 °C. The optical reflectance spectra of the CuSn nanotree oxide formed at 250 °C showed a 3–4% reflectance in the wavelength range between 400 and 900 nm, and its behavior differed from those of Cu and Sn oxides formed at 250 °C. The temperature dependence of electrical resistivity for the CuSn nanotree oxide showed a typical semiconductor behavior. By the introduction of H2, O2, N2, and CO gases into the chamber, the resistance of the CuSn nanotree oxide responded against H2 most sensitively, as well as against O2 and CO gases. From the resistance change tendency, it is strongly suggested that the CuSn nanotree oxide is a p-type semiconductor, because it shows an increase in conductivity caused by the adsorption of a negative charge such as O−. However, the conductivity decreases with the adsorption of a positive charge such as H+. The present study suggests the high potential of the CuSn nanotree oxide as a gas sensor, since it has a very high surface-to-volume ratio

Sample Entropy in Electrocardiogram During Atrial Fibrillation

【Background】 Atrial fibrillation (AF) is an arrhythmia commonly encountered in clinical practice. There is a high risk of thromboembolism in patients with AF. Nonlinear analyses such as electroencephalogram (EEG), electrocardiogram (ECG), and respiratory movement have been used to quantify biological signals, and sample entropy (SampEn) has been employed as a statistical measure to evaluate complex systems. In this study, we examined the values of SampEn in ECG signals for patients with and without AF to measure the regularity and complexity. 【Methods】 ECG signals of lead II were recorded from 34 subjects without arrhythmia and 15 patients with chronic AF in a supine position. The ECG signals were converted into time-series data and SampEn was calculated. 【Results】 The SampEn values for the group without arrhythmia were 0.252 ± 0.114 [time lag (τ) = 1] and 0.533 ± 0.163 (τ = 5), and those for the chronic AF group were 0.392 ± 0.158 (τ = 1) and 0.759 ± 0.246 (τ = 5). The values of SampEn were significantly higher in the group with chronic AF than in the group without arrhythmia (P < 0.01 for τ = 1, P < 0.004 for τ = 5). The constructed three-dimensional vectors were plotted in time-delayed three-dimensional space. We used time lags of τ = 5 and τ = 1. The shape of the loops of the three-dimensional space was better for τ = 5. 【Conclusion】 The values of SampEn from ECG for chronic AF patients were higher than for subjects without arrhythmia, suggesting greater complexity for the time-series from chronic AF patients. SampEn is considered a new index for evaluating complex systems in ECG