CASE STUDY ON ATTITUDE AND BEHAVIOR OF SCHOOL LUNCH OFFICIALS REGARDING UTILIZATION OF LOCAL PRODUCE -FOCUSING ON THE DIFFERENCE OF OCCUPATION

This study was conducted in January 2010 to identify influential factors for utilization of local produce in school lunch services and factor-correlations in terms of employment types and item types and to review the evaluation index for shokuiku. The research participants were 56 nutrition teachers, 91 school principals, and 23 school lunch directors. The study revealed that school lunch staff had high awareness and behavioral motivation for using local ingredients. School lunch directors were concerned about managerial aspects such as “high price” and “not enough variety and quantity” for making lunches with local ingredients only.  The principals expressed high opinion on educational or administrative advantages of using local ingredients in “increased number of local cuisine in the menu” and “securing food safety” but showed low awareness on problems related to using local produce to cook for entire student body such as “longer cooking time” and “not enough providers.” Correlation between questionnaire items revealed that there was a strong positive relation between items emphasizing advantages of using local produce. The results showed that using local produce for providing school lunches could induce changes in behaviors, such as frequenting local produce markets, and it is considered to become a good evaluation index for developing shokuiku

Arabidopsis ECHIDNA protein is involved in seed coloration, protein trafficking to vacuoles, and vacuolar biogenesis

Flavonoids are a major group of plant-specific metabolites that determine flower and seed coloration. In plant cells, flavonoids are synthesized at the cytosolic surface of the endoplasmic reticulum and are sequestered in the vacuole. It is possible that membrane trafficking, including vesicle trafficking and organelle dynamics, contributes to flavonoid transport and accumulation. However, the underlying mechanism has yet to be fully elucidated. Here we show that the Arabidopsis ECHIDNA protein plays a role in flavonoid accumulation in the vacuole and protein trafficking to the vacuole. We found defective pigmentation patterns in echidna seed, possibly caused by reduced levels of proanthocyanidins, which determine seed coloration. The echidna mutant has defects in protein sorting to the protein storage vacuole as well as vacuole morphology. These findings indicate that ECHIDNA is involved in the vacuolar trafficking pathway as well as the previously described secretory pathway. In addition, we found a genetic interaction between echidna and green fluorescent seed 9 (gfs9), a membrane trafficking factor involved in flavonoid accumulation. Our findings suggest that vacuolar trafficking and/or vacuolar development, both of which are collectively regulated by ECHIDNA and GFS9, are required for flavonoid accumulation, resulting in seed coat pigmentation

Stomagen positively regulates stomatal density in Arabidopsis.

葉の気孔の数を増加させる因子の発見～CO2削減や食糧増産へ向けて～. 京都大学プレスリリース. 2009-12-10.Stomata in the epidermal tissues of leaves are valves through which passes CO(2), and as such they influence the global carbon cycle. The two-dimensional pattern and density of stomata in the leaf epidermis are genetically and environmentally regulated to optimize gas exchange. Two putative intercellular signalling factors, EPF1 and EPF2, function as negative regulators of stomatal development in Arabidopsis, possibly by interacting with the receptor-like protein TMM. One or more positive intercellular signalling factors are assumed to be involved in stomatal development, but their identities are unknown. Here we show that a novel secretory peptide, which we designate as stomagen, is a positive intercellular signalling factor that is conserved among vascular plants. Stomagen is a 45-amino--rich peptide that is generated from a 102-amino-acid precursor protein designated as STOMAGEN. Both an in planta analysis and a semi-in-vitro analysis with recombinant and chemically synthesized stomagen peptides showed that stomagen has stomata-inducing activity in a dose-dependent manner. A genetic analysis showed that TMM is epistatic to STOMAGEN (At4g12970), suggesting that stomatal development is finely regulated by competitive binding of positive and negative regulators to the same receptor. Notably, STOMAGEN is expressed in inner tissues (the mesophyll) of immature leaves but not in the epidermal tissues where stomata develop. This study provides evidence of a mesophyll-derived positive regulator of stomatal density. Our findings provide a conceptual advancement in understanding stomatal development: inner photosynthetic tissues optimize their function by regulating stomatal density in the epidermis for efficient uptake of CO(2)

CASE STUDY ON ATTITUDE AND BEHAVIOR OF SCHOOL LUNCH OFFICIALS REGARDING UTILIZATION OF LOCAL PRODUCE -FOCUSING ON THE DIFFERENCE OF OCCUPATION

This study was conducted in January 2010 to identify influential factors for utilization of local produce in school lunch services and factor-correlations in terms of employment types and item types and to review the evaluation index for shokuiku. The research participants were 56 nutrition teachers, 91 school principals, and 23 school lunch directors. The study revealed that school lunch staff had high awareness and behavioral motivation for using local ingredients.School lunch directors were concerned about managerial aspects such as “high price” and “not enough variety and quantity” for making lunches with local ingredients only. The principals expressed high opinion on educational or administrative advantages of using local ingredients in “increased number of local cuisine in the menu” and “securing food safety” but showed low awareness on problems related to using local produce to cook for entire student body such as “longer cooking time” and “not enough providers.” Correlation between questionnaire items revealed that there was a strong positive relation between items emphasizing advantages of using local produce. The results showed that using local produce for providing school lunches could induce changes in behaviors, such as frequenting local produce markets, and it is considered to become a good evaluation index for developing shokuiku

Validity and reproducibility of food photographic estimation for evaluating meals in evacuation shelters

Objective: The primary goal was to evaluate the validity of food photographic estimation for nutritional assessment compared with weighed food record (WFR). Methods: We evaluated the validity and reproducibility of photographic estimation of foods provided in evacuation shelters. We analyzed 35 meals served at 12 shelters in Kumamoto Prefecture in Japan, affected by a heavy rain disaster in 2020. In this context, we compared 21 senior students' portion size estimation by food photographs to WFR. In addition, we assigned five meals for each of the 21 senior students, and the same meal photograph was estimated by three students to test reproducibility. Results: No statistically significant difference was detected between the two methods regarding energy, the total grams of meal, the protein, and vitamins B1, B2, and C, except for salt. In addition, the students who never self-cooked underestimated the total grams. Conclusion: Food photographic estimation could simplify the nutritional assessment in evacuation shelters. However, unclear photographs and food items served by weight could weaken the estimation accuracy. According to previous studies and the applied postestimation questionnaire, photographs taken from specified angles and reference food photobooks for portion size estimation may improve accuracy

Plant ESCRT protein ALIX coordinates with retromer complex in regulating receptor-mediated sorting of soluble vacuolar proteins

Vacuolar proteins play essential roles in plant physiology and development, but the factors and the machinery regulating their vesicle trafficking through the endomembrane compartments remain largely unknown. We and others have recently identified an evolutionarily conserved plant endosomal sorting complex required for transport (ESCRT)-associated protein apoptosis-linked gene-2 interacting protein X (ALIX), which plays canonical functions in the biogenesis of the multivesicular body/prevacuolar compartment (MVB/PVC) and in the sorting of ubiquitinated membrane proteins. In this study, we elucidate the roles and underlying mechanism of ALIX in regulating vacuolar transport of soluble proteins, beyond its conventional ESCRT function in eukaryotic cells. We show that ALIX colocalizes and physically interacts with the retromer core subunits Vps26 and Vps29 in planta. Moreover, double-mutant analysis reveals the genetic interaction of ALIX with Vps26 and Vps29 for regulating trafficking of soluble vacuolar proteins. Interestingly, depletion of ALIX perturbs membrane recruitment of Vps26 and Vps29 and alters the endosomal localization of vacuolar sorting receptors (VSRs). Taken together, ALIX functions as a unique retromer core subcomplex regulator by orchestrating receptor-mediated vacuolar sorting of soluble proteins.Peer reviewe

Review Article

Underlying values of the CCL4 spectrogram tested by two shortpass dichroic filters. Originally published in Applied Optics on 10 September 2016 (ao-55-26-7195

Efficient genome editing and its application to conditional genetic analysis in M. polymorpha

Marchantia polymorpha is one of the model species of basal land plants. Although CRISPR/ Cas9-based genome editing has already been demonstrated for this plant, the efficiency was too low to apply to functional analysis. In this study, we show the establishment of CRISPR/Cas9 genome editing vectors with high efficiency for both construction and genome editing. Codon optimization of Cas9 to Arabidopsis achieved over 70% genome editing efficiency at two loci tested. Systematic assessment revealed that guide sequences of 17 nt or shorter dramatically decreased this efficiency. We also demonstrated that a combinatorial use of this system and a floxed complementation construct enabled conditional analysis of a nearly essential gene. This study reports that simple, rapid, and efficient genome editing is feasible with the series of developed vectors